KLF5 promotes cell migration by up‐regulating FYN in bladder cancer cells
Krüppel‐like factor 5 (KLF5) promotes cell proliferation of bladder cancer. However, whether KLF5 regulates other cell processes in bladder cancer is not clear. We found that KLF5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cell...
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| Veröffentlicht in: | FEBS letters 2016-02, Vol.590 (3), p.408-418 |
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| creator | Du, Chong Gao, Yang Xu, Shan Jia, Jing Huang, Zhixin Fan, Jinhai Wang, Xinyang He, Dalin Guo, Peng Chen, Quan |
| description | Krüppel‐like factor 5 (KLF5) promotes cell proliferation of bladder cancer. However, whether KLF5 regulates other cell processes in bladder cancer is not clear. We found that KLF5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cells. In addition, KLF5 promotes transcription of FYN through binding to its promoter. FYN overexpression rescues cell migration and lamellipodia formation reduced by KLF5 knockdown. Furthermore, the KLF5/FYN/p‐FAK axis is necessary for lysophosphatidic acid (LPA) to promote cell migration. Our findings indicate that both KLF5 and FYN are important in the regulation of cell migration in bladder cancer cells. We propose the KLF5/FYN/p‐FAK axis as a potential therapeutic target in bladder cancer. |
| doi_str_mv | 10.1002/1873-3468.12069 |
| format | Article |
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However, whether KLF5 regulates other cell processes in bladder cancer is not clear. We found that KLF5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cells. In addition, KLF5 promotes transcription of FYN through binding to its promoter. FYN overexpression rescues cell migration and lamellipodia formation reduced by KLF5 knockdown. Furthermore, the KLF5/FYN/p‐FAK axis is necessary for lysophosphatidic acid (LPA) to promote cell migration. Our findings indicate that both KLF5 and FYN are important in the regulation of cell migration in bladder cancer cells. We propose the KLF5/FYN/p‐FAK axis as a potential therapeutic target in bladder cancer.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1002/1873-3468.12069</identifier><identifier>PMID: 26786295</identifier><language>eng</language><publisher>England</publisher><subject>bladder cancer ; Cell Line, Tumor ; cell migration ; Cell Movement ; FAK ; Focal Adhesion Kinase 1 - metabolism ; FYN ; Gene Expression Regulation, Neoplastic ; Humans ; KLF5 ; Kruppel-Like Transcription Factors - antagonists & inhibitors ; Kruppel-Like Transcription Factors - genetics ; Kruppel-Like Transcription Factors - metabolism ; Lysophospholipids - metabolism ; Microscopy, Confocal ; Microscopy, Fluorescence ; Microscopy, Video ; Neoplasm Proteins - agonists ; Neoplasm Proteins - genetics ; Neoplasm Proteins - metabolism ; Phosphorylation ; Promoter Regions, Genetic ; Protein Processing, Post-Translational ; Proto-Oncogene Proteins c-fyn - agonists ; Proto-Oncogene Proteins c-fyn - genetics ; Proto-Oncogene Proteins c-fyn - metabolism ; Pseudopodia - metabolism ; Pseudopodia - pathology ; Recombinant Proteins - chemistry ; Recombinant Proteins - metabolism ; RNA Interference ; Up-Regulation ; Urinary Bladder Neoplasms - metabolism ; Urinary Bladder Neoplasms - pathology</subject><ispartof>FEBS letters, 2016-02, Vol.590 (3), p.408-418</ispartof><rights>2016 Federation of European Biochemical Societies</rights><rights>2016 Federation of European Biochemical Societies.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1873-3468.12069$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1873-3468.12069$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26786295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Chong</creatorcontrib><creatorcontrib>Gao, Yang</creatorcontrib><creatorcontrib>Xu, Shan</creatorcontrib><creatorcontrib>Jia, Jing</creatorcontrib><creatorcontrib>Huang, Zhixin</creatorcontrib><creatorcontrib>Fan, Jinhai</creatorcontrib><creatorcontrib>Wang, Xinyang</creatorcontrib><creatorcontrib>He, Dalin</creatorcontrib><creatorcontrib>Guo, Peng</creatorcontrib><creatorcontrib>Chen, Quan</creatorcontrib><title>KLF5 promotes cell migration by up‐regulating FYN in bladder cancer cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Krüppel‐like factor 5 (KLF5) promotes cell proliferation of bladder cancer. However, whether KLF5 regulates other cell processes in bladder cancer is not clear. We found that KLF5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cells. In addition, KLF5 promotes transcription of FYN through binding to its promoter. FYN overexpression rescues cell migration and lamellipodia formation reduced by KLF5 knockdown. Furthermore, the KLF5/FYN/p‐FAK axis is necessary for lysophosphatidic acid (LPA) to promote cell migration. Our findings indicate that both KLF5 and FYN are important in the regulation of cell migration in bladder cancer cells. We propose the KLF5/FYN/p‐FAK axis as a potential therapeutic target in bladder cancer.</description><subject>bladder cancer</subject><subject>Cell Line, Tumor</subject><subject>cell migration</subject><subject>Cell Movement</subject><subject>FAK</subject><subject>Focal Adhesion Kinase 1 - metabolism</subject><subject>FYN</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>KLF5</subject><subject>Kruppel-Like Transcription Factors - antagonists & inhibitors</subject><subject>Kruppel-Like Transcription Factors - genetics</subject><subject>Kruppel-Like Transcription Factors - metabolism</subject><subject>Lysophospholipids - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Fluorescence</subject><subject>Microscopy, Video</subject><subject>Neoplasm Proteins - agonists</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Phosphorylation</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Processing, Post-Translational</subject><subject>Proto-Oncogene Proteins c-fyn - agonists</subject><subject>Proto-Oncogene Proteins c-fyn - genetics</subject><subject>Proto-Oncogene Proteins c-fyn - metabolism</subject><subject>Pseudopodia - metabolism</subject><subject>Pseudopodia - pathology</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><subject>RNA Interference</subject><subject>Up-Regulation</subject><subject>Urinary Bladder Neoplasms - metabolism</subject><subject>Urinary Bladder Neoplasms - pathology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kD1PwzAQhi0EoqUwsyGPLCk-O_4aoWoAtYIFBibLSZwqVT5K3Ahl4yfwG_klJG3p9N69997p9CB0DWQKhNA7UJIFLBRqCpQIfYLGR-cUjQmBMOBSsxG68H5N-l6BPkcjKqQSVPMxWiyWEcebpi7rrfM4cUWBy3zV2G1eVzjucLv5_f5p3Koteqta4ejjBef9pLBp6hqc2CoZpN_zl-gss4V3VwedoPdo_jZ7Cpavj8-z-2WwDhnXAUuI0prGaZZSTjU4pQRJNeWOCOk44VYTqanlVkCYJU5BHDquMkgo14wCm6Db_d3-7c_W-a0pcz98YCtXt96AFBwAtFR99OYQbePSpWbT5KVtOvMPoA-IfeArL1x3nAMxA18z0DQDTbPja6L5A91V7A8-1mst</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Du, Chong</creator><creator>Gao, Yang</creator><creator>Xu, Shan</creator><creator>Jia, Jing</creator><creator>Huang, Zhixin</creator><creator>Fan, Jinhai</creator><creator>Wang, Xinyang</creator><creator>He, Dalin</creator><creator>Guo, Peng</creator><creator>Chen, Quan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201602</creationdate><title>KLF5 promotes cell migration by up‐regulating FYN in bladder cancer cells</title><author>Du, Chong ; Gao, Yang ; Xu, Shan ; Jia, Jing ; Huang, Zhixin ; Fan, Jinhai ; Wang, Xinyang ; He, Dalin ; Guo, Peng ; Chen, Quan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j4359-3c08992bdfd25291e8860d925e067e505a90792a5a614fce81b4e58f1c2593213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>bladder cancer</topic><topic>Cell Line, Tumor</topic><topic>cell migration</topic><topic>Cell Movement</topic><topic>FAK</topic><topic>Focal Adhesion Kinase 1 - metabolism</topic><topic>FYN</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>KLF5</topic><topic>Kruppel-Like Transcription Factors - antagonists & inhibitors</topic><topic>Kruppel-Like Transcription Factors - genetics</topic><topic>Kruppel-Like Transcription Factors - metabolism</topic><topic>Lysophospholipids - metabolism</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Fluorescence</topic><topic>Microscopy, Video</topic><topic>Neoplasm Proteins - agonists</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Phosphorylation</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Processing, Post-Translational</topic><topic>Proto-Oncogene Proteins c-fyn - agonists</topic><topic>Proto-Oncogene Proteins c-fyn - genetics</topic><topic>Proto-Oncogene Proteins c-fyn - metabolism</topic><topic>Pseudopodia - metabolism</topic><topic>Pseudopodia - pathology</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - metabolism</topic><topic>RNA Interference</topic><topic>Up-Regulation</topic><topic>Urinary Bladder Neoplasms - metabolism</topic><topic>Urinary Bladder Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Chong</creatorcontrib><creatorcontrib>Gao, Yang</creatorcontrib><creatorcontrib>Xu, Shan</creatorcontrib><creatorcontrib>Jia, Jing</creatorcontrib><creatorcontrib>Huang, Zhixin</creatorcontrib><creatorcontrib>Fan, Jinhai</creatorcontrib><creatorcontrib>Wang, Xinyang</creatorcontrib><creatorcontrib>He, Dalin</creatorcontrib><creatorcontrib>Guo, Peng</creatorcontrib><creatorcontrib>Chen, Quan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Chong</au><au>Gao, Yang</au><au>Xu, Shan</au><au>Jia, Jing</au><au>Huang, Zhixin</au><au>Fan, Jinhai</au><au>Wang, Xinyang</au><au>He, Dalin</au><au>Guo, Peng</au><au>Chen, Quan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>KLF5 promotes cell migration by up‐regulating FYN in bladder cancer cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2016-02</date><risdate>2016</risdate><volume>590</volume><issue>3</issue><spage>408</spage><epage>418</epage><pages>408-418</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Krüppel‐like factor 5 (KLF5) promotes cell proliferation of bladder cancer. However, whether KLF5 regulates other cell processes in bladder cancer is not clear. We found that KLF5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cells. In addition, KLF5 promotes transcription of FYN through binding to its promoter. FYN overexpression rescues cell migration and lamellipodia formation reduced by KLF5 knockdown. Furthermore, the KLF5/FYN/p‐FAK axis is necessary for lysophosphatidic acid (LPA) to promote cell migration. Our findings indicate that both KLF5 and FYN are important in the regulation of cell migration in bladder cancer cells. We propose the KLF5/FYN/p‐FAK axis as a potential therapeutic target in bladder cancer.</abstract><cop>England</cop><pmid>26786295</pmid><doi>10.1002/1873-3468.12069</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | bladder cancer Cell Line, Tumor cell migration Cell Movement FAK Focal Adhesion Kinase 1 - metabolism FYN Gene Expression Regulation, Neoplastic Humans KLF5 Kruppel-Like Transcription Factors - antagonists & inhibitors Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism Lysophospholipids - metabolism Microscopy, Confocal Microscopy, Fluorescence Microscopy, Video Neoplasm Proteins - agonists Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Phosphorylation Promoter Regions, Genetic Protein Processing, Post-Translational Proto-Oncogene Proteins c-fyn - agonists Proto-Oncogene Proteins c-fyn - genetics Proto-Oncogene Proteins c-fyn - metabolism Pseudopodia - metabolism Pseudopodia - pathology Recombinant Proteins - chemistry Recombinant Proteins - metabolism RNA Interference Up-Regulation Urinary Bladder Neoplasms - metabolism Urinary Bladder Neoplasms - pathology |
| title | KLF5 promotes cell migration by up‐regulating FYN in bladder cancer cells |
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