Secretory expression, characterization and docking study of glucose-tolerant β-glucosidase from B. subtilis

The thermostable, glucose tolerant β-glucosidase gene (bgl) of Glycoside hydrolase family 1, isolated from Bacillus subtilis, was cloned and overexpressed in Escherichia coli. The bgl has open reading frame of 1407bp, encoding 469 amino acids with predicted molecular weight of 53kDa. The recombinant...

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Veröffentlicht in:	International journal of biological macromolecules 2016-04, Vol.85, p.425-433
Hauptverfasser:	Chamoli, Shivangi, Kumar, Piyush, Navani, Naveen Kumar, Verma, Ashok Kumar
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacillus subtilis - metabolism beta-Glucosidase - chemistry beta-Glucosidase - genetics beta-Glucosidase - isolation & purification beta-Glucosidase - metabolism Cloning, Molecular Enzyme Activation Enzyme Stability Extracellular expression Glucose - chemistry Glucose - metabolism Hydrogen-Ion Concentration Molecular docking Molecular Docking Simulation Molecular Sequence Data Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Alignment Sequence Analysis, DNA Substrate Specificity Temperature Thermostable-glucose tolerant β-glucosidase
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