MicroRNA-148a inhibits breast cancer migration and invasion by directly targeting WNT-1
Wnt/β-catenin signaling pathway influences embryonic development, cell polarity and adhesion, apoptosis and tumorigenesis. MicroRNAs (miRNAs) function as important regulators of the tumorigenesis and metastasis. In the present study, we aimed to find novel targets and mechanisms of microRNA-148a (mi...
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| Veröffentlicht in: | Oncology reports 2016-03, Vol.35 (3), p.1425-1432 |
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| creator | JIANG, QIAN HE, MIAO MA, MENG-TAO WU, HUI-ZHE YU, ZHAO-JIN GUAN, SHU JIANG, LONG-YANG WANG, YAN ZHENG, DA-DI JIN, FENG WEI, MIN-JIE |
| description | Wnt/β-catenin signaling pathway influences embryonic development, cell polarity and adhesion, apoptosis and tumorigenesis. MicroRNAs (miRNAs) function as important regulators of the tumorigenesis and metastasis. In the present study, we aimed to find novel targets and mechanisms of microRNA-148a (miR-148a) in regulating the migration and invasion of breast cancer cells. In the present study, miR-148a was found downregulated in human breast cancer tissues and cell lines. The ectopic miR-148a expression inhibited the migration and invasion of MCF-7 and MDA-MB-231 breast cancer cells. Furthermore, we demonstrated that WNT-1, one of the ligands of Wnt/β-catenin signaling pathway, was a direct target of miR-148a. The overexpression of miR-148a reduced the mRNA and protein expression levels of WNT-1, also decreased the expression levels of the key components of Wnt/β-catenin pathway, including β-catenin, metalloproteinase-7 (MMP-7) and T-cell factor-4 (TCF-4) in MCF-7 and MDA-MB-231 cells. In addition, the data showed that the expression of WNT-1 was significantly higher in human breast cancer tissues compared with the adjacent normal tissues and the expression of miR-148a was negatively correlated with the WNT-1 expression in human breast cancer tissues. Taken together, our results suggest that miR-148a can suppress the migration and invasion of breast cancer cells by targeting WNT-1 and inhibiting Wnt/β-catenin signaling pathway and this will provide new insights into the molecular mechanisms of breast cancer metastasis. |
| doi_str_mv | 10.3892/or.2015.4502 |
| format | Article |
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MicroRNAs (miRNAs) function as important regulators of the tumorigenesis and metastasis. In the present study, we aimed to find novel targets and mechanisms of microRNA-148a (miR-148a) in regulating the migration and invasion of breast cancer cells. In the present study, miR-148a was found downregulated in human breast cancer tissues and cell lines. The ectopic miR-148a expression inhibited the migration and invasion of MCF-7 and MDA-MB-231 breast cancer cells. Furthermore, we demonstrated that WNT-1, one of the ligands of Wnt/β-catenin signaling pathway, was a direct target of miR-148a. The overexpression of miR-148a reduced the mRNA and protein expression levels of WNT-1, also decreased the expression levels of the key components of Wnt/β-catenin pathway, including β-catenin, metalloproteinase-7 (MMP-7) and T-cell factor-4 (TCF-4) in MCF-7 and MDA-MB-231 cells. In addition, the data showed that the expression of WNT-1 was significantly higher in human breast cancer tissues compared with the adjacent normal tissues and the expression of miR-148a was negatively correlated with the WNT-1 expression in human breast cancer tissues. Taken together, our results suggest that miR-148a can suppress the migration and invasion of breast cancer cells by targeting WNT-1 and inhibiting Wnt/β-catenin signaling pathway and this will provide new insights into the molecular mechanisms of breast cancer metastasis.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2015.4502</identifier><identifier>PMID: 26707142</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Apoptosis ; beta Catenin - genetics ; Breast cancer ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cell growth ; Cell migration ; Cell Movement - genetics ; Development and progression ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Genetic regulation ; Health aspects ; Humans ; invasion ; Kinases ; MCF-7 Cells ; Metastasis ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; migration ; miR-148a ; Neoplasm Invasiveness - genetics ; Proteins ; RNA, Messenger - biosynthesis ; Rodents ; Studies ; Tumorigenesis ; Tumors ; Wnt Signaling Pathway - genetics ; WNT-1 ; Wnt/β-catenin signaling pathway ; Wnt1 Protein - genetics ; Wnt1 Protein - metabolism</subject><ispartof>Oncology reports, 2016-03, Vol.35 (3), p.1425-1432</ispartof><rights>Copyright © 2016, Spandidos Publications</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-d15fc0f7bb5e2a90edeabae06cbf4f4cc9480253089d320598dac2971de6f4f83</citedby><cites>FETCH-LOGICAL-c552t-d15fc0f7bb5e2a90edeabae06cbf4f4cc9480253089d320598dac2971de6f4f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26707142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JIANG, QIAN</creatorcontrib><creatorcontrib>HE, MIAO</creatorcontrib><creatorcontrib>MA, MENG-TAO</creatorcontrib><creatorcontrib>WU, HUI-ZHE</creatorcontrib><creatorcontrib>YU, ZHAO-JIN</creatorcontrib><creatorcontrib>GUAN, SHU</creatorcontrib><creatorcontrib>JIANG, LONG-YANG</creatorcontrib><creatorcontrib>WANG, YAN</creatorcontrib><creatorcontrib>ZHENG, DA-DI</creatorcontrib><creatorcontrib>JIN, FENG</creatorcontrib><creatorcontrib>WEI, MIN-JIE</creatorcontrib><title>MicroRNA-148a inhibits breast cancer migration and invasion by directly targeting WNT-1</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>Wnt/β-catenin signaling pathway influences embryonic development, cell polarity and adhesion, apoptosis and tumorigenesis. MicroRNAs (miRNAs) function as important regulators of the tumorigenesis and metastasis. In the present study, we aimed to find novel targets and mechanisms of microRNA-148a (miR-148a) in regulating the migration and invasion of breast cancer cells. In the present study, miR-148a was found downregulated in human breast cancer tissues and cell lines. The ectopic miR-148a expression inhibited the migration and invasion of MCF-7 and MDA-MB-231 breast cancer cells. Furthermore, we demonstrated that WNT-1, one of the ligands of Wnt/β-catenin signaling pathway, was a direct target of miR-148a. The overexpression of miR-148a reduced the mRNA and protein expression levels of WNT-1, also decreased the expression levels of the key components of Wnt/β-catenin pathway, including β-catenin, metalloproteinase-7 (MMP-7) and T-cell factor-4 (TCF-4) in MCF-7 and MDA-MB-231 cells. In addition, the data showed that the expression of WNT-1 was significantly higher in human breast cancer tissues compared with the adjacent normal tissues and the expression of miR-148a was negatively correlated with the WNT-1 expression in human breast cancer tissues. Taken together, our results suggest that miR-148a can suppress the migration and invasion of breast cancer cells by targeting WNT-1 and inhibiting Wnt/β-catenin signaling pathway and this will provide new insights into the molecular mechanisms of breast cancer metastasis.</description><subject>Apoptosis</subject><subject>beta Catenin - genetics</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Development and progression</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Genetic regulation</subject><subject>Health aspects</subject><subject>Humans</subject><subject>invasion</subject><subject>Kinases</subject><subject>MCF-7 Cells</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>migration</subject><subject>miR-148a</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Proteins</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Rodents</subject><subject>Studies</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>Wnt Signaling Pathway - genetics</subject><subject>WNT-1</subject><subject>Wnt/β-catenin signaling pathway</subject><subject>Wnt1 Protein - genetics</subject><subject>Wnt1 Protein - metabolism</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkUtr3DAQgE1paNK0t56LoVB6qLZ62tZxCX0E0hRKSnoTsjTeVbCljSQX9t9XJu9SdJBGfDMjzVdVbwhesU7STyGuKCZixQWmz6oj0kqCKGfkeTljShBj4vdh9TKlK4xpixv5ojqkTYtbwulRdfndmRh-nq8R4Z2und-63uVU9xF0yrXR3kCsJ7eJOrvga-1tgf7otAT9vrYugsnjvs46biA7v6kvzy8QeVUdDHpM8Pp2P65-ffl8cfINnf34enqyPkNGCJqRJWIweGj7XgDVEoMF3WvAjekHPnBjJO8wFQx30jKKheysNlS2xEJTgI4dVx9u6u5iuJ4hZTW5ZGActYcwJ0Xahjey41wW9N0_6FWYoy-vU0Qy2nQNFvSB2ugRlPNDyFGbpahac95yTLhY2q7-Q5VlYXImeBhcuX-S8P5Rwhb0mLcpjPMy1PQU_HgDFi0pRRjULrpJx70iWC3CVYhqEa4W4QV_e_upuZ_A3sN3hh8ap11x52xI90yIiAmEWVFfRvwXRN6vIA</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>JIANG, QIAN</creator><creator>HE, MIAO</creator><creator>MA, MENG-TAO</creator><creator>WU, HUI-ZHE</creator><creator>YU, ZHAO-JIN</creator><creator>GUAN, SHU</creator><creator>JIANG, LONG-YANG</creator><creator>WANG, YAN</creator><creator>ZHENG, DA-DI</creator><creator>JIN, FENG</creator><creator>WEI, MIN-JIE</creator><general>D.A. 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genetics</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Development and progression</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Genetic regulation</topic><topic>Health aspects</topic><topic>Humans</topic><topic>invasion</topic><topic>Kinases</topic><topic>MCF-7 Cells</topic><topic>Metastasis</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>migration</topic><topic>miR-148a</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Proteins</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Rodents</topic><topic>Studies</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><topic>Wnt Signaling Pathway - genetics</topic><topic>WNT-1</topic><topic>Wnt/β-catenin signaling pathway</topic><topic>Wnt1 Protein - 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Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JIANG, QIAN</au><au>HE, MIAO</au><au>MA, MENG-TAO</au><au>WU, HUI-ZHE</au><au>YU, ZHAO-JIN</au><au>GUAN, SHU</au><au>JIANG, LONG-YANG</au><au>WANG, YAN</au><au>ZHENG, DA-DI</au><au>JIN, FENG</au><au>WEI, MIN-JIE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-148a inhibits breast cancer migration and invasion by directly targeting WNT-1</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>35</volume><issue>3</issue><spage>1425</spage><epage>1432</epage><pages>1425-1432</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>Wnt/β-catenin signaling pathway influences embryonic development, cell polarity and adhesion, apoptosis and tumorigenesis. MicroRNAs (miRNAs) function as important regulators of the tumorigenesis and metastasis. In the present study, we aimed to find novel targets and mechanisms of microRNA-148a (miR-148a) in regulating the migration and invasion of breast cancer cells. In the present study, miR-148a was found downregulated in human breast cancer tissues and cell lines. The ectopic miR-148a expression inhibited the migration and invasion of MCF-7 and MDA-MB-231 breast cancer cells. Furthermore, we demonstrated that WNT-1, one of the ligands of Wnt/β-catenin signaling pathway, was a direct target of miR-148a. The overexpression of miR-148a reduced the mRNA and protein expression levels of WNT-1, also decreased the expression levels of the key components of Wnt/β-catenin pathway, including β-catenin, metalloproteinase-7 (MMP-7) and T-cell factor-4 (TCF-4) in MCF-7 and MDA-MB-231 cells. In addition, the data showed that the expression of WNT-1 was significantly higher in human breast cancer tissues compared with the adjacent normal tissues and the expression of miR-148a was negatively correlated with the WNT-1 expression in human breast cancer tissues. Taken together, our results suggest that miR-148a can suppress the migration and invasion of breast cancer cells by targeting WNT-1 and inhibiting Wnt/β-catenin signaling pathway and this will provide new insights into the molecular mechanisms of breast cancer metastasis.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>26707142</pmid><doi>10.3892/or.2015.4502</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Apoptosis beta Catenin - genetics Breast cancer Breast Neoplasms - genetics Breast Neoplasms - pathology Cell growth Cell migration Cell Movement - genetics Development and progression Female Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Genetic regulation Health aspects Humans invasion Kinases MCF-7 Cells Metastasis MicroRNA MicroRNAs MicroRNAs - genetics migration miR-148a Neoplasm Invasiveness - genetics Proteins RNA, Messenger - biosynthesis Rodents Studies Tumorigenesis Tumors Wnt Signaling Pathway - genetics WNT-1 Wnt/β-catenin signaling pathway Wnt1 Protein - genetics Wnt1 Protein - metabolism |
| title | MicroRNA-148a inhibits breast cancer migration and invasion by directly targeting WNT-1 |
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