Fungal denitrification: Bipolaris sorokiniana exclusively denitrifies inorganic nitrogen in the presence and absence of oxygen
Fungi may play an important role in the production of the greenhouse gas nitrous oxide (N2O). Bipolaris sorokiniana is a ubiquitous saprobe found in soils worldwide, yet denitrification by this fungal strain has not previously been reported. We aimed to test if B. sorokiniana would produce N2O and C...
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| Veröffentlicht in: | FEMS microbiology letters 2016-02, Vol.363 (4), p.fnw007 |
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| creator | Phillips, Rebecca Grelet, Gwen McMillan, Andrew Song, Bongkeun Weir, Bevan Palmada, Thilak Tobias, Craig |
| description | Fungi may play an important role in the production of the greenhouse gas nitrous oxide (N2O). Bipolaris sorokiniana is a ubiquitous saprobe found in soils worldwide, yet denitrification by this fungal strain has not previously been reported. We aimed to test if B. sorokiniana would produce N2O and CO2 in the presence of organic and inorganic forms of nitrogen (N) under microaerobic and anaerobic conditions. Nitrogen source (organic-N, inorganic-N, no-N control) significantly affected N2O and CO2 production both in the presence and absence of oxygen, which contrasts with bacterial denitrification. Inorganic N addition increased denitrification of N2O (from 0 to 0.3 μg N20-N h−1 g−1 biomass) and reduced respiration of CO2 (from 0.1 to 0.02 mg CO2 h−1 g−1 biomass). Isotope analyses indicated that nitrite, rather than ammonium or glutamine, was transformed to N2O. Results suggest the source of N may play a larger role in fungal N2O production than oxygen status.
Inorganic nitrogen addition alters fungal denitrification and respiration in the presence and absence of oxygen. |
| doi_str_mv | 10.1093/femsle/fnw007 |
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Inorganic nitrogen addition alters fungal denitrification and respiration in the presence and absence of oxygen.</description><identifier>ISSN: 1574-6968</identifier><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1093/femsle/fnw007</identifier><identifier>PMID: 26764425</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Ammonium ; Anaerobic conditions ; Ascomycota - metabolism ; Biomass ; Bipolaris sorokiniana ; Carbon dioxide ; Carbon Dioxide - metabolism ; Denitrification ; Fungi ; Glutamine ; Glutamine - metabolism ; Greenhouse effect ; Greenhouse gases ; Microbiology ; Nitric Oxide - metabolism ; Nitrogen ; Nitrogen - metabolism ; Nitrous oxide ; Nitrous Oxide - metabolism ; Oxygen ; Oxygen - metabolism</subject><ispartof>FEMS microbiology letters, 2016-02, Vol.363 (4), p.fnw007</ispartof><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2016</rights><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-2411235983c32a30df1390f57bf6f57a2d5882abda8dd07ffda045fd061266ea3</citedby><cites>FETCH-LOGICAL-c354t-2411235983c32a30df1390f57bf6f57a2d5882abda8dd07ffda045fd061266ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26764425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Brock, Matthias</contributor><creatorcontrib>Phillips, Rebecca</creatorcontrib><creatorcontrib>Grelet, Gwen</creatorcontrib><creatorcontrib>McMillan, Andrew</creatorcontrib><creatorcontrib>Song, Bongkeun</creatorcontrib><creatorcontrib>Weir, Bevan</creatorcontrib><creatorcontrib>Palmada, Thilak</creatorcontrib><creatorcontrib>Tobias, Craig</creatorcontrib><title>Fungal denitrification: Bipolaris sorokiniana exclusively denitrifies inorganic nitrogen in the presence and absence of oxygen</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Fungi may play an important role in the production of the greenhouse gas nitrous oxide (N2O). Bipolaris sorokiniana is a ubiquitous saprobe found in soils worldwide, yet denitrification by this fungal strain has not previously been reported. We aimed to test if B. sorokiniana would produce N2O and CO2 in the presence of organic and inorganic forms of nitrogen (N) under microaerobic and anaerobic conditions. Nitrogen source (organic-N, inorganic-N, no-N control) significantly affected N2O and CO2 production both in the presence and absence of oxygen, which contrasts with bacterial denitrification. Inorganic N addition increased denitrification of N2O (from 0 to 0.3 μg N20-N h−1 g−1 biomass) and reduced respiration of CO2 (from 0.1 to 0.02 mg CO2 h−1 g−1 biomass). Isotope analyses indicated that nitrite, rather than ammonium or glutamine, was transformed to N2O. Results suggest the source of N may play a larger role in fungal N2O production than oxygen status.
Inorganic nitrogen addition alters fungal denitrification and respiration in the presence and absence of oxygen.</description><subject>Ammonium</subject><subject>Anaerobic conditions</subject><subject>Ascomycota - metabolism</subject><subject>Biomass</subject><subject>Bipolaris sorokiniana</subject><subject>Carbon dioxide</subject><subject>Carbon Dioxide - metabolism</subject><subject>Denitrification</subject><subject>Fungi</subject><subject>Glutamine</subject><subject>Glutamine - metabolism</subject><subject>Greenhouse effect</subject><subject>Greenhouse gases</subject><subject>Microbiology</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitrogen</subject><subject>Nitrogen - metabolism</subject><subject>Nitrous oxide</subject><subject>Nitrous Oxide - metabolism</subject><subject>Oxygen</subject><subject>Oxygen - metabolism</subject><issn>1574-6968</issn><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc1P3DAQxa2qqCy0x16RpV56CfgrTpZbi_iSVuIC52g2Hm-9ZO1gJy174W_HKLSLuPQyMxr95ulpHiFfOTvmbC5PLG5ShyfW_2Gs-kBmvKxUoee6_vhm3icHKa0ZY0ow_YnsC11ppUQ5I08Xo19BRw16N0RnXQuDC_6U_nR96CC6RFOI4d55Bx4oPrbdmNxv7La7E0zU-RBX4F1LX3ZhhT6v6PALaR8xoW-RgjcUltMcLA2P20x9JnsWuoRfXvshubs4vz27KhY3l9dnPxZFK0s1FEJxLmQ5r2UrBUhmLJdzZstqaXWuIExZ1wKWBmpjWGWtAaZKa5jmQmsEeUi-T7p9DA8jpqHZuNRi14HHMKaG539kSVHxjH57h67DGH121wglOROKKZWpYqLaGFKKaJs-ug3EbcNZ8xJMMwXTTMFk_uhVdVxu0Pyj_yaxcxjG_j9az4w_m38</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Phillips, Rebecca</creator><creator>Grelet, Gwen</creator><creator>McMillan, Andrew</creator><creator>Song, Bongkeun</creator><creator>Weir, Bevan</creator><creator>Palmada, Thilak</creator><creator>Tobias, Craig</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160201</creationdate><title>Fungal denitrification: Bipolaris sorokiniana exclusively denitrifies inorganic nitrogen in the presence and absence of oxygen</title><author>Phillips, Rebecca ; Grelet, Gwen ; McMillan, Andrew ; Song, Bongkeun ; Weir, Bevan ; Palmada, Thilak ; Tobias, Craig</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-2411235983c32a30df1390f57bf6f57a2d5882abda8dd07ffda045fd061266ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Ammonium</topic><topic>Anaerobic conditions</topic><topic>Ascomycota - metabolism</topic><topic>Biomass</topic><topic>Bipolaris sorokiniana</topic><topic>Carbon dioxide</topic><topic>Carbon Dioxide - metabolism</topic><topic>Denitrification</topic><topic>Fungi</topic><topic>Glutamine</topic><topic>Glutamine - metabolism</topic><topic>Greenhouse effect</topic><topic>Greenhouse gases</topic><topic>Microbiology</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitrogen</topic><topic>Nitrogen - metabolism</topic><topic>Nitrous oxide</topic><topic>Nitrous Oxide - metabolism</topic><topic>Oxygen</topic><topic>Oxygen - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phillips, Rebecca</creatorcontrib><creatorcontrib>Grelet, Gwen</creatorcontrib><creatorcontrib>McMillan, Andrew</creatorcontrib><creatorcontrib>Song, Bongkeun</creatorcontrib><creatorcontrib>Weir, Bevan</creatorcontrib><creatorcontrib>Palmada, Thilak</creatorcontrib><creatorcontrib>Tobias, Craig</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phillips, Rebecca</au><au>Grelet, Gwen</au><au>McMillan, Andrew</au><au>Song, Bongkeun</au><au>Weir, Bevan</au><au>Palmada, Thilak</au><au>Tobias, Craig</au><au>Brock, Matthias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fungal denitrification: Bipolaris sorokiniana exclusively denitrifies inorganic nitrogen in the presence and absence of oxygen</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>363</volume><issue>4</issue><spage>fnw007</spage><pages>fnw007-</pages><issn>1574-6968</issn><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Fungi may play an important role in the production of the greenhouse gas nitrous oxide (N2O). Bipolaris sorokiniana is a ubiquitous saprobe found in soils worldwide, yet denitrification by this fungal strain has not previously been reported. We aimed to test if B. sorokiniana would produce N2O and CO2 in the presence of organic and inorganic forms of nitrogen (N) under microaerobic and anaerobic conditions. Nitrogen source (organic-N, inorganic-N, no-N control) significantly affected N2O and CO2 production both in the presence and absence of oxygen, which contrasts with bacterial denitrification. Inorganic N addition increased denitrification of N2O (from 0 to 0.3 μg N20-N h−1 g−1 biomass) and reduced respiration of CO2 (from 0.1 to 0.02 mg CO2 h−1 g−1 biomass). Isotope analyses indicated that nitrite, rather than ammonium or glutamine, was transformed to N2O. Results suggest the source of N may play a larger role in fungal N2O production than oxygen status.
Inorganic nitrogen addition alters fungal denitrification and respiration in the presence and absence of oxygen.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>26764425</pmid><doi>10.1093/femsle/fnw007</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Ammonium Anaerobic conditions Ascomycota - metabolism Biomass Bipolaris sorokiniana Carbon dioxide Carbon Dioxide - metabolism Denitrification Fungi Glutamine Glutamine - metabolism Greenhouse effect Greenhouse gases Microbiology Nitric Oxide - metabolism Nitrogen Nitrogen - metabolism Nitrous oxide Nitrous Oxide - metabolism Oxygen Oxygen - metabolism |
| title | Fungal denitrification: Bipolaris sorokiniana exclusively denitrifies inorganic nitrogen in the presence and absence of oxygen |
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