NF-κB mediates the antiproliferative and proapoptotic effects of bergamot juice in HepG2 cells
Among cancers, hepatocellular carcinoma is one of the commonest worldwide, and its incidence is increasing around the world. A lot of evidence underlines that natural substances usually consumed in the diet can have an important role in the prevention of cancer. In this study we investigated the mol...
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| Veröffentlicht in: | Life sciences (1973) 2016-02, Vol.146, p.81-91 |
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| container_title | Life sciences (1973) |
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| creator | Ferlazzo, Nadia Cirmi, Santa Russo, Marina Trapasso, Elena Ursino, Maria Rita Lombardo, Giovanni Enrico Gangemi, Sebastiano Calapai, Gioacchino Navarra, Michele |
| description | Among cancers, hepatocellular carcinoma is one of the commonest worldwide, and its incidence is increasing around the world. A lot of evidence underlines that natural substances usually consumed in the diet can have an important role in the prevention of cancer. In this study we investigated the molecular mechanisms underlying the antiproliferative activity of Citrus bergamia (bergamot) juice (BJ) in human hepatocellular carcinoma HepG2 cells.
HepG2 cells were exposed to BJ and then cell proliferation, cell cycle progression, apoptosis and NF-κB nuclear translocation were evaluated.
Here we present results demonstrating that BJ reduced the growth rate of human hepatocellular carcinoma HepG2 cells in a time- and concentration-dependent manner, by a mechanism involving the activation of apoptotic machinery via both intrinsic and extrinsic pathways. Moreover, BJ increased expression of P53 and P21 proteins that may be responsible for the HepG2 cell cycle arrest in G2 phase. In addition, BJ reduced NF-κB nuclear translocation.
Our data demonstrate the ability of BJ in reducing the growth of HepG2 cells, revealing its mechanism of action and suggesting a promising role as anticancer drugs. |
| doi_str_mv | 10.1016/j.lfs.2015.12.040 |
| format | Article |
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HepG2 cells were exposed to BJ and then cell proliferation, cell cycle progression, apoptosis and NF-κB nuclear translocation were evaluated.
Here we present results demonstrating that BJ reduced the growth rate of human hepatocellular carcinoma HepG2 cells in a time- and concentration-dependent manner, by a mechanism involving the activation of apoptotic machinery via both intrinsic and extrinsic pathways. Moreover, BJ increased expression of P53 and P21 proteins that may be responsible for the HepG2 cell cycle arrest in G2 phase. In addition, BJ reduced NF-κB nuclear translocation.
Our data demonstrate the ability of BJ in reducing the growth of HepG2 cells, revealing its mechanism of action and suggesting a promising role as anticancer drugs.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2015.12.040</identifier><identifier>PMID: 26764233</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Antiproliferative activity ; Apoptosis ; Apoptosis - drug effects ; Cell Cycle - drug effects ; Cell Proliferation - drug effects ; Citrus bergamia ; Comet Assay ; Complementary medicine ; DNA Damage ; DNA, Complementary - biosynthesis ; DNA, Complementary - genetics ; Flavonoids ; G2 Phase - drug effects ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Expression Regulation, Neoplastic - genetics ; Genes, p53 - drug effects ; Hep G2 Cells ; HepG2 cells ; Heterocyclic Compounds - chemistry ; Humans ; Natural products ; NF-kappa B - metabolism ; Oncogene Protein p21(ras) - drug effects ; Oncogene Protein p21(ras) - genetics ; Plant Extracts - pharmacology ; RNA, Neoplasm - biosynthesis ; Translocation, Genetic</subject><ispartof>Life sciences (1973), 2016-02, Vol.146, p.81-91</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-166cb4655bdac42fd3d048d39ff65df300b688e36fc71db56c7b9b5506592c83</citedby><cites>FETCH-LOGICAL-c353t-166cb4655bdac42fd3d048d39ff65df300b688e36fc71db56c7b9b5506592c83</cites><orcidid>0000-0002-6492-7820</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2015.12.040$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26764233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferlazzo, Nadia</creatorcontrib><creatorcontrib>Cirmi, Santa</creatorcontrib><creatorcontrib>Russo, Marina</creatorcontrib><creatorcontrib>Trapasso, Elena</creatorcontrib><creatorcontrib>Ursino, Maria Rita</creatorcontrib><creatorcontrib>Lombardo, Giovanni Enrico</creatorcontrib><creatorcontrib>Gangemi, Sebastiano</creatorcontrib><creatorcontrib>Calapai, Gioacchino</creatorcontrib><creatorcontrib>Navarra, Michele</creatorcontrib><title>NF-κB mediates the antiproliferative and proapoptotic effects of bergamot juice in HepG2 cells</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Among cancers, hepatocellular carcinoma is one of the commonest worldwide, and its incidence is increasing around the world. A lot of evidence underlines that natural substances usually consumed in the diet can have an important role in the prevention of cancer. In this study we investigated the molecular mechanisms underlying the antiproliferative activity of Citrus bergamia (bergamot) juice (BJ) in human hepatocellular carcinoma HepG2 cells.
HepG2 cells were exposed to BJ and then cell proliferation, cell cycle progression, apoptosis and NF-κB nuclear translocation were evaluated.
Here we present results demonstrating that BJ reduced the growth rate of human hepatocellular carcinoma HepG2 cells in a time- and concentration-dependent manner, by a mechanism involving the activation of apoptotic machinery via both intrinsic and extrinsic pathways. Moreover, BJ increased expression of P53 and P21 proteins that may be responsible for the HepG2 cell cycle arrest in G2 phase. In addition, BJ reduced NF-κB nuclear translocation.
Our data demonstrate the ability of BJ in reducing the growth of HepG2 cells, revealing its mechanism of action and suggesting a promising role as anticancer drugs.</description><subject>Antiproliferative activity</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Citrus bergamia</subject><subject>Comet Assay</subject><subject>Complementary medicine</subject><subject>DNA Damage</subject><subject>DNA, Complementary - biosynthesis</subject><subject>DNA, Complementary - genetics</subject><subject>Flavonoids</subject><subject>G2 Phase - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Genes, p53 - drug effects</subject><subject>Hep G2 Cells</subject><subject>HepG2 cells</subject><subject>Heterocyclic Compounds - chemistry</subject><subject>Humans</subject><subject>Natural products</subject><subject>NF-kappa B - metabolism</subject><subject>Oncogene Protein p21(ras) - drug effects</subject><subject>Oncogene Protein p21(ras) - genetics</subject><subject>Plant Extracts - pharmacology</subject><subject>RNA, Neoplasm - biosynthesis</subject><subject>Translocation, Genetic</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu2zAQRImiQeOk_YBeCh57kbIkRUpCT6mR2AGC9JI7QZHLloZsqSRtIL-Wj-g3hYaTHHtaYDAzmH2EfGVQM2DqalOPPtUcmKwZr6GBD2TBuravQAn2kSwAeFMJDvKcXKS0AQApW_GJnHPVqoYLsSD64bb69_yTbtEFkzHR_Aep2eUwx2kMHqPJ4XBUHC2Kmac5TzlYit6jzYlOng4Yf5vtlOlmHyzSsKNrnFecWhzH9JmceTMm_PJ6L8nj7c3jcl3d_1rdLa_vKyukyBVTyg6NknJwxjbcO-Gg6ZzovVfSeQEwqK5DobxtmRuksu3QD1KCkj23nbgk30-1ZeTfPaastyEdB5gdTvukWfmXia7nvFjZyWrjlFJEr-cYtiY-aQb6iFVvdMGqj1g147pgLZlvr_X7oZB6T7xxLIYfJwOWHw8Bo0424M4WqrFw0m4K_6l_AdnBiP4</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Ferlazzo, Nadia</creator><creator>Cirmi, Santa</creator><creator>Russo, Marina</creator><creator>Trapasso, Elena</creator><creator>Ursino, Maria Rita</creator><creator>Lombardo, Giovanni Enrico</creator><creator>Gangemi, Sebastiano</creator><creator>Calapai, Gioacchino</creator><creator>Navarra, Michele</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6492-7820</orcidid></search><sort><creationdate>20160201</creationdate><title>NF-κB mediates the antiproliferative and proapoptotic effects of bergamot juice in HepG2 cells</title><author>Ferlazzo, Nadia ; 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A lot of evidence underlines that natural substances usually consumed in the diet can have an important role in the prevention of cancer. In this study we investigated the molecular mechanisms underlying the antiproliferative activity of Citrus bergamia (bergamot) juice (BJ) in human hepatocellular carcinoma HepG2 cells.
HepG2 cells were exposed to BJ and then cell proliferation, cell cycle progression, apoptosis and NF-κB nuclear translocation were evaluated.
Here we present results demonstrating that BJ reduced the growth rate of human hepatocellular carcinoma HepG2 cells in a time- and concentration-dependent manner, by a mechanism involving the activation of apoptotic machinery via both intrinsic and extrinsic pathways. Moreover, BJ increased expression of P53 and P21 proteins that may be responsible for the HepG2 cell cycle arrest in G2 phase. In addition, BJ reduced NF-κB nuclear translocation.
Our data demonstrate the ability of BJ in reducing the growth of HepG2 cells, revealing its mechanism of action and suggesting a promising role as anticancer drugs.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>26764233</pmid><doi>10.1016/j.lfs.2015.12.040</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6492-7820</orcidid></addata></record> |
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| subjects | Antiproliferative activity Apoptosis Apoptosis - drug effects Cell Cycle - drug effects Cell Proliferation - drug effects Citrus bergamia Comet Assay Complementary medicine DNA Damage DNA, Complementary - biosynthesis DNA, Complementary - genetics Flavonoids G2 Phase - drug effects Gene Expression Regulation, Neoplastic - drug effects Gene Expression Regulation, Neoplastic - genetics Genes, p53 - drug effects Hep G2 Cells HepG2 cells Heterocyclic Compounds - chemistry Humans Natural products NF-kappa B - metabolism Oncogene Protein p21(ras) - drug effects Oncogene Protein p21(ras) - genetics Plant Extracts - pharmacology RNA, Neoplasm - biosynthesis Translocation, Genetic |
| title | NF-κB mediates the antiproliferative and proapoptotic effects of bergamot juice in HepG2 cells |
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