Degradation of organic pollutants by methane grown microbial consortia
Microbial consortia were enriched from various environmental samples with methane as the sole carbon and energy source. Selected consortia that showed a capacity for co-oxidation of naphthalene were screened for their ability to degrade methyl-tert-butyl-ether (MTBE), phthalic acid esters (PAE), ben...
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| Veröffentlicht in: | Biodegradation (Dordrecht) 2005-10, Vol.16 (5), p.435-448 |
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| creator | Hesselsoe, M Boysen, S Iversen, N Jørgensen, L Murrell, J.C McDonald, I Radajewski, S Thestrup, H Roslev, P |
| description | Microbial consortia were enriched from various environmental samples with methane as the sole carbon and energy source. Selected consortia that showed a capacity for co-oxidation of naphthalene were screened for their ability to degrade methyl-tert-butyl-ether (MTBE), phthalic acid esters (PAE), benzene, xylene and toluene (BTX). MTBE was not removed within 24 h by any of the consortia examined. One consortium enriched from activated sludge ("AAE-A2"), degraded PAE, including (butyl-benzyl)phthalate (BBP), and di-(butyl)phthalate (DBP). PAE have not previously been described as substrates for methanotrophic consortia. The apparent Km and Vmax for DBP degradation by AAE-A2 at 20 degrees C was 3.1 +/- 1.2 mg l(-1) and 8.7 +/- 1.1 mg DBP (g protein x h)(-1), respectively. AAE-A2 also showed fast degradation of BTX (230 +/- 30 nmol benzene (mg protein x h)(-1) at 20 degrees C). Additionally, AAE-A2 degraded benzene continuously for 2 weeks. In contrast, a pure culture of the methanotroph Methylosinus trichosporium OB3b ceased benzene degradation after only 2 days. Experiments with methane mono-oxygenase inhibitors or competitive substrates suggested that BTX degradation was carried out by methane-oxidizing bacteria in the consortium, whereas the degradation of PAE was carried out by non-methanotrophic bacteria co-existing with methanotrophs. The composition of the consortium (AAE-A2) based on polar lipid fatty acid (PLFA) profiles showed dominance of type II methanotrophs (83-92% of biomass). Phylogeny based on a 16S-rRNA gene clone library revealed that the dominating methanotrophs belonged to Methylosinus/Methylocystis spp. and that members of at least 4 different non-methanotrophic genera were present (Pseudomonas, Flavobacterium, Janthinobacterium and Rubivivax). |
| doi_str_mv | 10.1007/s10532-004-4721-2 |
| format | Article |
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Selected consortia that showed a capacity for co-oxidation of naphthalene were screened for their ability to degrade methyl-tert-butyl-ether (MTBE), phthalic acid esters (PAE), benzene, xylene and toluene (BTX). MTBE was not removed within 24 h by any of the consortia examined. One consortium enriched from activated sludge ("AAE-A2"), degraded PAE, including (butyl-benzyl)phthalate (BBP), and di-(butyl)phthalate (DBP). PAE have not previously been described as substrates for methanotrophic consortia. The apparent Km and Vmax for DBP degradation by AAE-A2 at 20 degrees C was 3.1 +/- 1.2 mg l(-1) and 8.7 +/- 1.1 mg DBP (g protein x h)(-1), respectively. AAE-A2 also showed fast degradation of BTX (230 +/- 30 nmol benzene (mg protein x h)(-1) at 20 degrees C). Additionally, AAE-A2 degraded benzene continuously for 2 weeks. In contrast, a pure culture of the methanotroph Methylosinus trichosporium OB3b ceased benzene degradation after only 2 days. Experiments with methane mono-oxygenase inhibitors or competitive substrates suggested that BTX degradation was carried out by methane-oxidizing bacteria in the consortium, whereas the degradation of PAE was carried out by non-methanotrophic bacteria co-existing with methanotrophs. The composition of the consortium (AAE-A2) based on polar lipid fatty acid (PLFA) profiles showed dominance of type II methanotrophs (83-92% of biomass). Phylogeny based on a 16S-rRNA gene clone library revealed that the dominating methanotrophs belonged to Methylosinus/Methylocystis spp. and that members of at least 4 different non-methanotrophic genera were present (Pseudomonas, Flavobacterium, Janthinobacterium and Rubivivax).</description><identifier>ISSN: 0923-9820</identifier><identifier>EISSN: 1572-9729</identifier><identifier>DOI: 10.1007/s10532-004-4721-2</identifier><identifier>PMID: 15865157</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Activated sludge ; Bacteria ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteria - metabolism ; Benzene ; Benzene Derivatives - metabolism ; Biodegradation of pollutants ; Biodegradation, Environmental ; Biological and medical sciences ; Biotechnology ; Consortia ; Degradation ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Energy sources ; Environment and pollution ; Environmental Microbiology ; Environmental Pollutants - metabolism ; Esters ; Feedback ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Industrial applications and implications. Economical aspects ; Kinetics ; Methane ; Methane - metabolism ; Methyl Ethers - metabolism ; Microbiology ; Molecular Sequence Data ; Naphthalene ; Organic Chemicals - metabolism ; Oxidation-Reduction ; Phthalates ; Phthalic Acids - metabolism ; Phylogeny ; Sewage - microbiology ; Temperature ; Toluene ; Xylene</subject><ispartof>Biodegradation (Dordrecht), 2005-10, Vol.16 (5), p.435-448</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-f67a57d0d08115be117ecac35930db730582661e189763d05e57399c79753c323</citedby><cites>FETCH-LOGICAL-c442t-f67a57d0d08115be117ecac35930db730582661e189763d05e57399c79753c323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16719736$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15865157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hesselsoe, M</creatorcontrib><creatorcontrib>Boysen, S</creatorcontrib><creatorcontrib>Iversen, N</creatorcontrib><creatorcontrib>Jørgensen, L</creatorcontrib><creatorcontrib>Murrell, J.C</creatorcontrib><creatorcontrib>McDonald, I</creatorcontrib><creatorcontrib>Radajewski, S</creatorcontrib><creatorcontrib>Thestrup, H</creatorcontrib><creatorcontrib>Roslev, P</creatorcontrib><title>Degradation of organic pollutants by methane grown microbial consortia</title><title>Biodegradation (Dordrecht)</title><addtitle>Biodegradation</addtitle><description>Microbial consortia were enriched from various environmental samples with methane as the sole carbon and energy source. Selected consortia that showed a capacity for co-oxidation of naphthalene were screened for their ability to degrade methyl-tert-butyl-ether (MTBE), phthalic acid esters (PAE), benzene, xylene and toluene (BTX). MTBE was not removed within 24 h by any of the consortia examined. One consortium enriched from activated sludge ("AAE-A2"), degraded PAE, including (butyl-benzyl)phthalate (BBP), and di-(butyl)phthalate (DBP). PAE have not previously been described as substrates for methanotrophic consortia. The apparent Km and Vmax for DBP degradation by AAE-A2 at 20 degrees C was 3.1 +/- 1.2 mg l(-1) and 8.7 +/- 1.1 mg DBP (g protein x h)(-1), respectively. AAE-A2 also showed fast degradation of BTX (230 +/- 30 nmol benzene (mg protein x h)(-1) at 20 degrees C). Additionally, AAE-A2 degraded benzene continuously for 2 weeks. In contrast, a pure culture of the methanotroph Methylosinus trichosporium OB3b ceased benzene degradation after only 2 days. Experiments with methane mono-oxygenase inhibitors or competitive substrates suggested that BTX degradation was carried out by methane-oxidizing bacteria in the consortium, whereas the degradation of PAE was carried out by non-methanotrophic bacteria co-existing with methanotrophs. The composition of the consortium (AAE-A2) based on polar lipid fatty acid (PLFA) profiles showed dominance of type II methanotrophs (83-92% of biomass). Phylogeny based on a 16S-rRNA gene clone library revealed that the dominating methanotrophs belonged to Methylosinus/Methylocystis spp. and that members of at least 4 different non-methanotrophic genera were present (Pseudomonas, Flavobacterium, Janthinobacterium and Rubivivax).</description><subject>Activated sludge</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Benzene</subject><subject>Benzene Derivatives - metabolism</subject><subject>Biodegradation of pollutants</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Consortia</subject><subject>Degradation</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Energy sources</subject><subject>Environment and pollution</subject><subject>Environmental Microbiology</subject><subject>Environmental Pollutants - metabolism</subject><subject>Esters</subject><subject>Feedback</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Kinetics</subject><subject>Methane</subject><subject>Methane - metabolism</subject><subject>Methyl Ethers - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Naphthalene</subject><subject>Organic Chemicals - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Phthalates</subject><subject>Phthalic Acids - metabolism</subject><subject>Phylogeny</subject><subject>Sewage - microbiology</subject><subject>Temperature</subject><subject>Toluene</subject><subject>Xylene</subject><issn>0923-9820</issn><issn>1572-9729</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0U1v1DAQBmALgejS9gdwgQgJbqEz_pr4WBUKSJU4tD1bjuMsqZJ4sRNV_ff1aleqxAVffHlmNDMvY-8RviIAXWQEJXgNIGtJHGv-im1QEa8NcfOabcBwUZuGwwl7l_MDABgC_padoGq0KnLDrr-FbXKdW4Y4V7GvYtq6efDVLo7jurh5yVX7VE1h-ePmUG1TfJyrafAptoMbKx_nHNMyuDP2pndjDufH_5TdX3-_u_pZ3_z-8evq8qb2UvKl7jU5RR100CCqNiBS8M4LZQR0LQlQDdcaAzaGtOhABUXCGE-GlPCCi1P25dB3l-LfNeTFTkP2YRzLdHHNFkuZRoP_h5JUA3IPP_0DH-Ka5rKEJcm1FCRlQXhAZfGcU-jtLg2TS08Wwe6jsIcobInC7qOw-1E_HBuv7RS6l4rj7Qv4fAQuezf2yc1-yC9OExoSuriPB9e7aN02FXN_ywEFYHlaN-IZxF2X5g</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Hesselsoe, M</creator><creator>Boysen, S</creator><creator>Iversen, N</creator><creator>Jørgensen, L</creator><creator>Murrell, J.C</creator><creator>McDonald, I</creator><creator>Radajewski, S</creator><creator>Thestrup, H</creator><creator>Roslev, P</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>SOI</scope></search><sort><creationdate>20051001</creationdate><title>Degradation of organic pollutants by methane grown microbial consortia</title><author>Hesselsoe, M ; Boysen, S ; Iversen, N ; Jørgensen, L ; Murrell, J.C ; McDonald, I ; Radajewski, S ; Thestrup, H ; Roslev, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-f67a57d0d08115be117ecac35930db730582661e189763d05e57399c79753c323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Activated sludge</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - metabolism</topic><topic>Benzene</topic><topic>Benzene Derivatives - metabolism</topic><topic>Biodegradation of pollutants</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Consortia</topic><topic>Degradation</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Energy sources</topic><topic>Environment and pollution</topic><topic>Environmental Microbiology</topic><topic>Environmental Pollutants - metabolism</topic><topic>Esters</topic><topic>Feedback</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Industrial applications and implications. 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(Dordrecht)</jtitle><addtitle>Biodegradation</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>16</volume><issue>5</issue><spage>435</spage><epage>448</epage><pages>435-448</pages><issn>0923-9820</issn><eissn>1572-9729</eissn><abstract>Microbial consortia were enriched from various environmental samples with methane as the sole carbon and energy source. Selected consortia that showed a capacity for co-oxidation of naphthalene were screened for their ability to degrade methyl-tert-butyl-ether (MTBE), phthalic acid esters (PAE), benzene, xylene and toluene (BTX). MTBE was not removed within 24 h by any of the consortia examined. One consortium enriched from activated sludge ("AAE-A2"), degraded PAE, including (butyl-benzyl)phthalate (BBP), and di-(butyl)phthalate (DBP). PAE have not previously been described as substrates for methanotrophic consortia. The apparent Km and Vmax for DBP degradation by AAE-A2 at 20 degrees C was 3.1 +/- 1.2 mg l(-1) and 8.7 +/- 1.1 mg DBP (g protein x h)(-1), respectively. AAE-A2 also showed fast degradation of BTX (230 +/- 30 nmol benzene (mg protein x h)(-1) at 20 degrees C). Additionally, AAE-A2 degraded benzene continuously for 2 weeks. In contrast, a pure culture of the methanotroph Methylosinus trichosporium OB3b ceased benzene degradation after only 2 days. Experiments with methane mono-oxygenase inhibitors or competitive substrates suggested that BTX degradation was carried out by methane-oxidizing bacteria in the consortium, whereas the degradation of PAE was carried out by non-methanotrophic bacteria co-existing with methanotrophs. The composition of the consortium (AAE-A2) based on polar lipid fatty acid (PLFA) profiles showed dominance of type II methanotrophs (83-92% of biomass). Phylogeny based on a 16S-rRNA gene clone library revealed that the dominating methanotrophs belonged to Methylosinus/Methylocystis spp. and that members of at least 4 different non-methanotrophic genera were present (Pseudomonas, Flavobacterium, Janthinobacterium and Rubivivax).</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>15865157</pmid><doi>10.1007/s10532-004-4721-2</doi><tpages>14</tpages></addata></record> |
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| ispartof | Biodegradation (Dordrecht), 2005-10, Vol.16 (5), p.435-448 |
| issn | 0923-9820 1572-9729 |
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| subjects | Activated sludge Bacteria Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Benzene Benzene Derivatives - metabolism Biodegradation of pollutants Biodegradation, Environmental Biological and medical sciences Biotechnology Consortia Degradation DNA, Bacterial - genetics DNA, Ribosomal - genetics Energy sources Environment and pollution Environmental Microbiology Environmental Pollutants - metabolism Esters Feedback Fundamental and applied biological sciences. Psychology Genes, Bacterial Industrial applications and implications. Economical aspects Kinetics Methane Methane - metabolism Methyl Ethers - metabolism Microbiology Molecular Sequence Data Naphthalene Organic Chemicals - metabolism Oxidation-Reduction Phthalates Phthalic Acids - metabolism Phylogeny Sewage - microbiology Temperature Toluene Xylene |
| title | Degradation of organic pollutants by methane grown microbial consortia |
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