Fidelity analysis of HIV‐1 reverse transcriptase mutants with an altered amino‐acid sequence at residues Leu74, Glu89, Tyr115, Tyr183 and Met184

Substitution of particular residues postulated to have a role in active site architecture can alter the overall fidelity of DNA polymerization by HIV‐1. The effects of this kind of substitution were determined in a lacZ‐based assay using HIV‐1 reverse transcriptase with specifically mutated residues...

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