Fidelity analysis of HIV‐1 reverse transcriptase mutants with an altered amino‐acid sequence at residues Leu74, Glu89, Tyr115, Tyr183 and Met184

Substitution of particular residues postulated to have a role in active site architecture can alter the overall fidelity of DNA polymerization by HIV‐1. The effects of this kind of substitution were determined in a lacZ‐based assay using HIV‐1 reverse transcriptase with specifically mutated residues...

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Veröffentlicht in:European journal of biochemistry 2000-05, Vol.267 (9), p.2658-2665
Hauptverfasser: Jonckheere, Heidi, De Clercq, Erik, Anné, Jozef
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De Clercq, Erik
Anné, Jozef
description Substitution of particular residues postulated to have a role in active site architecture can alter the overall fidelity of DNA polymerization by HIV‐1. The effects of this kind of substitution were determined in a lacZ‐based assay using HIV‐1 reverse transcriptase with specifically mutated residues. We found that the reported higher fidelity of nucleotide incorporation by the Met184→Val and Glu89→Gly mutant reverse transcriptases (RTs) was not reflected in a substantial increase in the overall fidelity for these RT mutants. For the 3TC‐resistant Met184→Val RT mutant an almost wild‐type level of overall mutation frequency was observed, while the foscarnet‐resistant RTs harbouring the Glu89→Gly mutation showed only a twofold decrease in mutation frequency. The Tyr183→Phe mutant RT displayed a slightly lower fidelity than wild‐type RT. Conversely, the ddI‐resistant RT mutant containing the Leu74→Val mutation showed a 3.5‐fold higher fidelity compared to the wild‐type enzyme. Finally, the Tyr115→Ala substitution rendered the enzyme substantially more error‐prone for DNA polymerization. These results correlate with three‐dimensional structural studies of the polymerase active site and confirm the postulated impact of the Leu74, Tyr183 and Tyr115 RT residues on the overall fidelity of DNA polymerization by HIV‐1 RT.
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source MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection
subjects Amino Acid Substitution
Amino Acids - chemistry
Amino Acids - genetics
Base Sequence
Biopolymers
fidelity
HIV Reverse Transcriptase - chemistry
HIV Reverse Transcriptase - genetics
HIV Reverse Transcriptase - metabolism
HIV‐1 RT
Human immunodeficiency virus 1
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
mutant reverse transcriptase
polymerase fidelity
polymerization
Sequence Homology, Nucleic Acid
site‐directed mutagenesis
title Fidelity analysis of HIV‐1 reverse transcriptase mutants with an altered amino‐acid sequence at residues Leu74, Glu89, Tyr115, Tyr183 and Met184
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