A Change in the Apparent m Value Reveals a Populated Intermediate under Equilibrium Conditions in Escherichia coli Ribonuclease HI

Experimental studies of protein stability often rely on the determination of an “m value”, which describes the denaturant dependence of the free energy change between two states (ΔG = ΔG H 2 O − m[denaturant]). Changes in the m value accompanying site specific mutations are usually attributed to str...

Ausführliche Beschreibung