Evaluation of VIDAS Listeria monocytogenes II (LMO2) immunoassay method for the detection of Listeria monocytogenes in foods: collaborative study
A multilaboratory study was conducted to compare the VIDAS Listeria monocytogenes II (LMO2) immunoassay and the standard cultural methods for the detection of Listeria monocytogenes in foods. Five food types-vanilla ice cream, brie cheese, cooked roast beef, frozen green beans, and frozen tilapia fi...
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| Veröffentlicht in: | Journal of AOAC International 2004-09, Vol.87 (5), p.1123-1132 |
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| container_title | Journal of AOAC International |
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| creator | Silbernagel, K.M Carver, C.N Jechorek, R.P Johnson, R.L |
| description | A multilaboratory study was conducted to compare the VIDAS Listeria monocytogenes II (LMO2) immunoassay and the standard cultural methods for the detection of Listeria monocytogenes in foods. Five food types-vanilla ice cream, brie cheese, cooked roast beef, frozen green beans, and frozen tilapia fish-at 3 levels were analyzed by each method. A total of 26 laboratories representing government and industry participated. In this study, 1404 test portions were analyzed of which 1152 were used in the statistical analysis. There were 448 positive by the VIDAS LMO2 assay and 457 positive by the standard culture methods. A chi2 analysis of each of the 5 food types, at the 3 inoculation levels tested, was performed. The resulting chi2 value, 0.36, indicates that overall, there are no statistical differences between the VIDAS LMO2 assay and the standard methods at the 5% level of significance. |
| doi_str_mv | 10.1093/jaoac/87.5.1123 |
| format | Article |
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Five food types-vanilla ice cream, brie cheese, cooked roast beef, frozen green beans, and frozen tilapia fish-at 3 levels were analyzed by each method. A total of 26 laboratories representing government and industry participated. In this study, 1404 test portions were analyzed of which 1152 were used in the statistical analysis. There were 448 positive by the VIDAS LMO2 assay and 457 positive by the standard culture methods. A chi2 analysis of each of the 5 food types, at the 3 inoculation levels tested, was performed. The resulting chi2 value, 0.36, indicates that overall, there are no statistical differences between the VIDAS LMO2 assay and the standard methods at the 5% level of significance.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.1093/jaoac/87.5.1123</identifier><identifier>PMID: 15493669</identifier><language>eng</language><publisher>Gaithersburg, MD: AOAC International</publisher><subject>bacterial contamination ; beef ; Biological and medical sciences ; Brie cheese ; enzyme-linked fluorescent immunoassay ; food contamination ; Food industries ; Food Microbiology ; food pathogens ; frozen fish ; frozen vegetables ; Fundamental and applied biological sciences. Psychology ; General aspects ; green beans ; ice cream ; Immunoassay ; immunoassays ; Listeria monocytogenes ; Listeria monocytogenes - isolation & purification ; Methods of analysis, processing and quality control, regulation, standards ; roasts ; tilapia (common name) ; VIDAS Listeria monocytogenes II (LMO2) immunoassay</subject><ispartof>Journal of AOAC International, 2004-09, Vol.87 (5), p.1123-1132</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-4faba2fd56c8387a0557b1b6e09ac60f4c690543088a16643e0da98cb2cefdfb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16120200$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15493669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silbernagel, K.M</creatorcontrib><creatorcontrib>Carver, C.N</creatorcontrib><creatorcontrib>Jechorek, R.P</creatorcontrib><creatorcontrib>Johnson, R.L</creatorcontrib><creatorcontrib>Collaborators</creatorcontrib><title>Evaluation of VIDAS Listeria monocytogenes II (LMO2) immunoassay method for the detection of Listeria monocytogenes in foods: collaborative study</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>A multilaboratory study was conducted to compare the VIDAS Listeria monocytogenes II (LMO2) immunoassay and the standard cultural methods for the detection of Listeria monocytogenes in foods. Five food types-vanilla ice cream, brie cheese, cooked roast beef, frozen green beans, and frozen tilapia fish-at 3 levels were analyzed by each method. A total of 26 laboratories representing government and industry participated. In this study, 1404 test portions were analyzed of which 1152 were used in the statistical analysis. There were 448 positive by the VIDAS LMO2 assay and 457 positive by the standard culture methods. A chi2 analysis of each of the 5 food types, at the 3 inoculation levels tested, was performed. The resulting chi2 value, 0.36, indicates that overall, there are no statistical differences between the VIDAS LMO2 assay and the standard methods at the 5% level of significance.</description><subject>bacterial contamination</subject><subject>beef</subject><subject>Biological and medical sciences</subject><subject>Brie cheese</subject><subject>enzyme-linked fluorescent immunoassay</subject><subject>food contamination</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>frozen fish</subject><subject>frozen vegetables</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>green beans</subject><subject>ice cream</subject><subject>Immunoassay</subject><subject>immunoassays</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>roasts</subject><subject>tilapia (common name)</subject><subject>VIDAS Listeria monocytogenes II (LMO2) immunoassay</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U1v1DAQBmALgWgpnLmBLyA4ZHf8ETvmVpUCKy3qoZSrNXHsNlUSF9uptD-Df0zKLuqJ0_jw-NVoXkJeM1gxMGJ9ixHdutGresUYF0_IMTNSVtpw_nR5g4JKcM2OyIucbwEkU8CfkyNWSyOUMsfk9_k9DjOWPk40Bvpz8_n0km77XHzqkY5xim5X4rWffKabDf2w_X7BP9J-HOcpYs64o6MvN7GjISZabjztfPHuX9x_gvpp4bHLn6iLw4BtTMsC957mMne7l-RZwCH7V4d5Qq6-nP84-1ZtL75uzk63lZNNXSoZsEUeulq5RjQaoa51y1rlwaBTEKRTBmopoGmQKSWFhw5N41rufOhCK07I-33uXYq_Zp-LHfvs_LLP5OOcLdNKCC34Atd76FLMOflg71I_YtpZBvahBfu3BdtoW9uHFpYfbw7Rczv67tEfzr6AdweA2eEQEk6uz49OMQ4cYHFv9y5gtHidFnN1yYEJAKMbzUH8AasknF8</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Silbernagel, K.M</creator><creator>Carver, C.N</creator><creator>Jechorek, R.P</creator><creator>Johnson, R.L</creator><general>AOAC International</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7T7</scope><scope>7U2</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20040901</creationdate><title>Evaluation of VIDAS Listeria monocytogenes II (LMO2) immunoassay method for the detection of Listeria monocytogenes in foods: collaborative study</title><author>Silbernagel, K.M ; Carver, C.N ; Jechorek, R.P ; Johnson, R.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-4faba2fd56c8387a0557b1b6e09ac60f4c690543088a16643e0da98cb2cefdfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>bacterial contamination</topic><topic>beef</topic><topic>Biological and medical sciences</topic><topic>Brie cheese</topic><topic>enzyme-linked fluorescent immunoassay</topic><topic>food contamination</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>frozen fish</topic><topic>frozen vegetables</topic><topic>Fundamental and applied biological sciences. 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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | bacterial contamination beef Biological and medical sciences Brie cheese enzyme-linked fluorescent immunoassay food contamination Food industries Food Microbiology food pathogens frozen fish frozen vegetables Fundamental and applied biological sciences. Psychology General aspects green beans ice cream Immunoassay immunoassays Listeria monocytogenes Listeria monocytogenes - isolation & purification Methods of analysis, processing and quality control, regulation, standards roasts tilapia (common name) VIDAS Listeria monocytogenes II (LMO2) immunoassay |
| title | Evaluation of VIDAS Listeria monocytogenes II (LMO2) immunoassay method for the detection of Listeria monocytogenes in foods: collaborative study |
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