Molecular characterization of the SCAR markers tightly linked to the Tm-2 locus of the genus Lycopersicon
The Tm-2 gene and its alleles conferring tomato mosaic virus resistance in tomato originate from Lycopersicon peruvianum, a wild relative of tomato. DNA fragments of several RAPD markers tightly linked with the Tm-2 locus in tomato were successfully cloned and sequenced. Subsequently, the 24-mer oli...
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| Veröffentlicht in: | Theoretical and applied genetics 2000-07, Vol.101 (1-2), p.64-69 |
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| creator | SOBIR OHMORI, T MURATA, M MOTOYOSHI, F |
| description | The Tm-2 gene and its alleles conferring tomato mosaic virus resistance in tomato originate from Lycopersicon peruvianum, a wild relative of tomato. DNA fragments of several RAPD markers tightly linked with the Tm-2 locus in tomato were successfully cloned and sequenced. Subsequently, the 24-mer oligonucleotide primer pairs of the SCAR markers corresponding to the RAPD markers were designed based on the 5'-endmost sequences. A fragment of the same size as that of a SCAR marker was amplified in the ToMV-susceptible tomato line with no Tm-2, but the digests of the PCR fragments by ^4ccI exhibited polymorphism in fragment length between the two lines. We chose three SCAR markers and three RAPD markers tightly linked with the Tm-2 locus, and examined whether the same-sized fragments corresponding to these markers were also present in three other lines carrying Tm-2a or one of the other Tm-2 alleles. The fragments corresponding to the three SCAR markers were present in all of the three lines, but the other markers (three RAPDs) were absent in one or two lines, suggesting that the three SCAR markers are closer to Tm-2 than the other markers. Comparison of the nucleotide sequences of these fragments revealed that they are all homologous to the corresponding SCAR markers. |
| doi_str_mv | 10.1007/s001220051450 |
| format | Article |
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DNA fragments of several RAPD markers tightly linked with the Tm-2 locus in tomato were successfully cloned and sequenced. Subsequently, the 24-mer oligonucleotide primer pairs of the SCAR markers corresponding to the RAPD markers were designed based on the 5'-endmost sequences. A fragment of the same size as that of a SCAR marker was amplified in the ToMV-susceptible tomato line with no Tm-2, but the digests of the PCR fragments by ^4ccI exhibited polymorphism in fragment length between the two lines. We chose three SCAR markers and three RAPD markers tightly linked with the Tm-2 locus, and examined whether the same-sized fragments corresponding to these markers were also present in three other lines carrying Tm-2a or one of the other Tm-2 alleles. The fragments corresponding to the three SCAR markers were present in all of the three lines, but the other markers (three RAPDs) were absent in one or two lines, suggesting that the three SCAR markers are closer to Tm-2 than the other markers. Comparison of the nucleotide sequences of these fragments revealed that they are all homologous to the corresponding SCAR markers.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220051450</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Analysis ; Biological and medical sciences ; Cloning ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic aspects ; Genetic markers ; Genetic testing ; Genetics and breeding of economic plants ; Identification and classification ; Lycopersicon ; Pest resistance ; Plant genetics ; Plant immunology ; Plant pathogens ; Tm-2 gene ; Tomato mosaic virus ; Tomatoes ; Varietal selection. 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DNA fragments of several RAPD markers tightly linked with the Tm-2 locus in tomato were successfully cloned and sequenced. Subsequently, the 24-mer oligonucleotide primer pairs of the SCAR markers corresponding to the RAPD markers were designed based on the 5'-endmost sequences. A fragment of the same size as that of a SCAR marker was amplified in the ToMV-susceptible tomato line with no Tm-2, but the digests of the PCR fragments by ^4ccI exhibited polymorphism in fragment length between the two lines. We chose three SCAR markers and three RAPD markers tightly linked with the Tm-2 locus, and examined whether the same-sized fragments corresponding to these markers were also present in three other lines carrying Tm-2a or one of the other Tm-2 alleles. The fragments corresponding to the three SCAR markers were present in all of the three lines, but the other markers (three RAPDs) were absent in one or two lines, suggesting that the three SCAR markers are closer to Tm-2 than the other markers. Comparison of the nucleotide sequences of these fragments revealed that they are all homologous to the corresponding SCAR markers.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Cloning</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic markers</subject><subject>Genetic testing</subject><subject>Genetics and breeding of economic plants</subject><subject>Identification and classification</subject><subject>Lycopersicon</subject><subject>Pest resistance</subject><subject>Plant genetics</subject><subject>Plant immunology</subject><subject>Plant pathogens</subject><subject>Tm-2 gene</subject><subject>Tomato mosaic virus</subject><subject>Tomatoes</subject><subject>Varietal selection. 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Soil science and plant productions</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Cloning</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic markers</topic><topic>Genetic testing</topic><topic>Genetics and breeding of economic plants</topic><topic>Identification and classification</topic><topic>Lycopersicon</topic><topic>Pest resistance</topic><topic>Plant genetics</topic><topic>Plant immunology</topic><topic>Plant pathogens</topic><topic>Tm-2 gene</topic><topic>Tomato mosaic virus</topic><topic>Tomatoes</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SOBIR</creatorcontrib><creatorcontrib>OHMORI, T</creatorcontrib><creatorcontrib>MURATA, M</creatorcontrib><creatorcontrib>MOTOYOSHI, F</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SOBIR</au><au>OHMORI, T</au><au>MURATA, M</au><au>MOTOYOSHI, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of the SCAR markers tightly linked to the Tm-2 locus of the genus Lycopersicon</atitle><jtitle>Theoretical and applied genetics</jtitle><date>2000-07-01</date><risdate>2000</risdate><volume>101</volume><issue>1-2</issue><spage>64</spage><epage>69</epage><pages>64-69</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>The Tm-2 gene and its alleles conferring tomato mosaic virus resistance in tomato originate from Lycopersicon peruvianum, a wild relative of tomato. DNA fragments of several RAPD markers tightly linked with the Tm-2 locus in tomato were successfully cloned and sequenced. Subsequently, the 24-mer oligonucleotide primer pairs of the SCAR markers corresponding to the RAPD markers were designed based on the 5'-endmost sequences. A fragment of the same size as that of a SCAR marker was amplified in the ToMV-susceptible tomato line with no Tm-2, but the digests of the PCR fragments by ^4ccI exhibited polymorphism in fragment length between the two lines. We chose three SCAR markers and three RAPD markers tightly linked with the Tm-2 locus, and examined whether the same-sized fragments corresponding to these markers were also present in three other lines carrying Tm-2a or one of the other Tm-2 alleles. The fragments corresponding to the three SCAR markers were present in all of the three lines, but the other markers (three RAPDs) were absent in one or two lines, suggesting that the three SCAR markers are closer to Tm-2 than the other markers. Comparison of the nucleotide sequences of these fragments revealed that they are all homologous to the corresponding SCAR markers.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220051450</doi><tpages>6</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Analysis Biological and medical sciences Cloning Fundamental and applied biological sciences. Psychology Genes Genetic aspects Genetic markers Genetic testing Genetics and breeding of economic plants Identification and classification Lycopersicon Pest resistance Plant genetics Plant immunology Plant pathogens Tm-2 gene Tomato mosaic virus Tomatoes Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Molecular characterization of the SCAR markers tightly linked to the Tm-2 locus of the genus Lycopersicon |
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