Molecular characterization of Vibrio parahaemolyticus vSGLT: a model for sodium-coupled sugar cotransporters

The Na(+)/galactose cotransporter (vSGLT) of Vibrio parahaemolyticus, tagged with C-terminal hexahistidine, has been purified to apparent homogeneity by Ni(2+) affinity chromatography and gel filtration. Resequencing the vSGLT gene identified an important correction: the N terminus constitutes an ad...

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Veröffentlicht in:	The Journal of biological chemistry 2000-08, Vol.275 (33), p.25711-25716
Hauptverfasser:	Turk, E, Kim, O, le Coutre, J, Whitelegge, J P, Eskandari, S, Lam, J T, Kreman, M, Zampighi, G, Faull, K F, Wright, E M
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological Transport Electrophoresis, Polyacrylamide Gel Freeze Fracturing galactose Galactose - pharmacokinetics Green Fluorescent Proteins Histidine - metabolism Kinetics Luminescent Proteins - metabolism Mass Spectrometry Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - isolation & purification Microscopy, Electron Molecular Sequence Data Monosaccharide Transport Proteins - chemistry Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - isolation & purification Na+/galactose cotransporter Plasmids - metabolism Promoter Regions, Genetic Proteolipids - metabolism Proteolipids - ultrastructure Recombinant Fusion Proteins - metabolism Sodium - metabolism Sodium-Glucose Transporter 1 Time Factors Vibrio parahaemolyticus Vibrio parahaemolyticus - chemistry vSGLT gene
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