Transcriptome analysis of rat liver regeneration in a model of oval hepatic stem cells
We have performed serial analysis of gene expression of the regenerating liver. In the rat model of partial hepatectomy and 2-acetamidofluorene treatment liver regeneration recruits hepatic stem cells referred to as oval cells. We analyzed a total of 153,057 tags in livers from normal control (52,34...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2005-09, Vol.86 (3), p.352-364 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Cimica, Velasco Batusic, Danko Chen, Yonglong Hollemann, Thomas Pieler, Tomas Ramadori, Giuliano |
| description | We have performed serial analysis of gene expression of the regenerating liver. In the rat model of partial hepatectomy and 2-acetamidofluorene treatment liver regeneration recruits hepatic stem cells referred to as oval cells. We analyzed a total of 153,057 tags in livers from normal control (52,343 tags), from sham 2-acetamidofluorene-treated control (50,502 tags), and from the early stage of oval cell proliferation (50,212 tags). Comparative analysis of the three transcriptomes identified 27 up-regulated and 18 down-regulated genes. Real-time PCR analysis confirmed 11 temporally regulated genes that correlate with oval cell development. Interestingly, we found by Western blot protein analysis of regenerating livers that the cell cycle gene Cdc42 was induced concomitant with the proliferation marker cyclin D1 and the oval cell marker alpha-fetoprotein. Our studies provide new insights into the molecular mechanism of liver regeneration through oval cells. |
| doi_str_mv | 10.1016/j.ygeno.2005.05.001 |
| format | Article |
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In the rat model of partial hepatectomy and 2-acetamidofluorene treatment liver regeneration recruits hepatic stem cells referred to as oval cells. We analyzed a total of 153,057 tags in livers from normal control (52,343 tags), from sham 2-acetamidofluorene-treated control (50,502 tags), and from the early stage of oval cell proliferation (50,212 tags). Comparative analysis of the three transcriptomes identified 27 up-regulated and 18 down-regulated genes. Real-time PCR analysis confirmed 11 temporally regulated genes that correlate with oval cell development. Interestingly, we found by Western blot protein analysis of regenerating livers that the cell cycle gene Cdc42 was induced concomitant with the proliferation marker cyclin D1 and the oval cell marker alpha-fetoprotein. Our studies provide new insights into the molecular mechanism of liver regeneration through oval cells.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2005.05.001</identifier><identifier>PMID: 15993033</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>2-Acetylaminofluorene - pharmacology ; alpha-Fetoproteins - genetics ; Animals ; Biological and medical sciences ; Blood Proteins - genetics ; cdc42 GTP-Binding Protein - genetics ; Down-Regulation ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation ; Genes. Genome ; Genetics of eukaryotes. Biological and molecular evolution ; Hepatocytes - cytology ; Hepatocytes - metabolism ; Liver - drug effects ; Liver regeneration ; Liver Regeneration - genetics ; Molecular and cellular biology ; Molecular genetics ; Oval cells ; Partial hepatectomy ; Rat liver ; Rats ; RNA, Messenger - analysis ; RNA, Messenger - metabolism ; Serial analysis of gene expression ; Stem Cells - metabolism ; Transcription, Genetic ; Transcriptome ; Up-Regulation</subject><ispartof>Genomics (San Diego, Calif.), 2005-09, Vol.86 (3), p.352-364</ispartof><rights>2005 Elsevier Inc.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-e081a4030d19243a134b345d721cc41d49fa69179f98f0828e83f0400b8c35813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ygeno.2005.05.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17032203$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15993033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cimica, Velasco</creatorcontrib><creatorcontrib>Batusic, Danko</creatorcontrib><creatorcontrib>Chen, Yonglong</creatorcontrib><creatorcontrib>Hollemann, Thomas</creatorcontrib><creatorcontrib>Pieler, Tomas</creatorcontrib><creatorcontrib>Ramadori, Giuliano</creatorcontrib><title>Transcriptome analysis of rat liver regeneration in a model of oval hepatic stem cells</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>We have performed serial analysis of gene expression of the regenerating liver. In the rat model of partial hepatectomy and 2-acetamidofluorene treatment liver regeneration recruits hepatic stem cells referred to as oval cells. We analyzed a total of 153,057 tags in livers from normal control (52,343 tags), from sham 2-acetamidofluorene-treated control (50,502 tags), and from the early stage of oval cell proliferation (50,212 tags). Comparative analysis of the three transcriptomes identified 27 up-regulated and 18 down-regulated genes. Real-time PCR analysis confirmed 11 temporally regulated genes that correlate with oval cell development. Interestingly, we found by Western blot protein analysis of regenerating livers that the cell cycle gene Cdc42 was induced concomitant with the proliferation marker cyclin D1 and the oval cell marker alpha-fetoprotein. Our studies provide new insights into the molecular mechanism of liver regeneration through oval cells.</description><subject>2-Acetylaminofluorene - pharmacology</subject><subject>alpha-Fetoproteins - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Proteins - genetics</subject><subject>cdc42 GTP-Binding Protein - genetics</subject><subject>Down-Regulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genes. Genome</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver regeneration</subject><subject>Liver Regeneration - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Oval cells</subject><subject>Partial hepatectomy</subject><subject>Rat liver</subject><subject>Rats</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>Serial analysis of gene expression</subject><subject>Stem Cells - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcriptome</subject><subject>Up-Regulation</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlZ_gSC56G3rZJPdTQ4epPgFBS_Va0izs5qyu6nJttB_764t9CYMDGGevDM8hFwzmDJg-f1quvvC1k9TgGw6FLATMmYgVSJzkZ-SMUgpkyITfEQuYlwBgOIyPScjlinFgfMx-VwE00Yb3LrzDVLTmnoXXaS-osF0tHZbDDRgvwj7t_MtdS01tPEl1gPkt6am37juZ5bGDhtqsa7jJTmrTB3x6tAn5OP5aTF7TebvL2-zx3liBZNdgiCZEcChZCoV3DAullxkZZEy2xOlUJXJFStUpWQFMpUoeQUCYCktzyTjE3K3z10H_7PB2OnGxeEC06LfRM2KPFUFkz3I96ANPsaAlV4H15iw0wz0oFOv9J9OPejUQ8EQf3OI3ywbLI9_Dv564PYAmGhNXfUyrYtHrgCepjBwD3sOexlbh0FH67C1WLqAttOld_8e8gstypM_</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Cimica, Velasco</creator><creator>Batusic, Danko</creator><creator>Chen, Yonglong</creator><creator>Hollemann, Thomas</creator><creator>Pieler, Tomas</creator><creator>Ramadori, Giuliano</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20050901</creationdate><title>Transcriptome analysis of rat liver regeneration in a model of oval hepatic stem cells</title><author>Cimica, Velasco ; Batusic, Danko ; Chen, Yonglong ; Hollemann, Thomas ; Pieler, Tomas ; Ramadori, Giuliano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-e081a4030d19243a134b345d721cc41d49fa69179f98f0828e83f0400b8c35813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>2-Acetylaminofluorene - pharmacology</topic><topic>alpha-Fetoproteins - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Proteins - genetics</topic><topic>cdc42 GTP-Binding Protein - genetics</topic><topic>Down-Regulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Genes. Genome</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver regeneration</topic><topic>Liver Regeneration - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Oval cells</topic><topic>Partial hepatectomy</topic><topic>Rat liver</topic><topic>Rats</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - metabolism</topic><topic>Serial analysis of gene expression</topic><topic>Stem Cells - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcriptome</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cimica, Velasco</creatorcontrib><creatorcontrib>Batusic, Danko</creatorcontrib><creatorcontrib>Chen, Yonglong</creatorcontrib><creatorcontrib>Hollemann, Thomas</creatorcontrib><creatorcontrib>Pieler, Tomas</creatorcontrib><creatorcontrib>Ramadori, Giuliano</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cimica, Velasco</au><au>Batusic, Danko</au><au>Chen, Yonglong</au><au>Hollemann, Thomas</au><au>Pieler, Tomas</au><au>Ramadori, Giuliano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis of rat liver regeneration in a model of oval hepatic stem cells</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>86</volume><issue>3</issue><spage>352</spage><epage>364</epage><pages>352-364</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>We have performed serial analysis of gene expression of the regenerating liver. In the rat model of partial hepatectomy and 2-acetamidofluorene treatment liver regeneration recruits hepatic stem cells referred to as oval cells. We analyzed a total of 153,057 tags in livers from normal control (52,343 tags), from sham 2-acetamidofluorene-treated control (50,502 tags), and from the early stage of oval cell proliferation (50,212 tags). Comparative analysis of the three transcriptomes identified 27 up-regulated and 18 down-regulated genes. Real-time PCR analysis confirmed 11 temporally regulated genes that correlate with oval cell development. Interestingly, we found by Western blot protein analysis of regenerating livers that the cell cycle gene Cdc42 was induced concomitant with the proliferation marker cyclin D1 and the oval cell marker alpha-fetoprotein. Our studies provide new insights into the molecular mechanism of liver regeneration through oval cells.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>15993033</pmid><doi>10.1016/j.ygeno.2005.05.001</doi><tpages>13</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | 2-Acetylaminofluorene - pharmacology alpha-Fetoproteins - genetics Animals Biological and medical sciences Blood Proteins - genetics cdc42 GTP-Binding Protein - genetics Down-Regulation Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation Genes. Genome Genetics of eukaryotes. Biological and molecular evolution Hepatocytes - cytology Hepatocytes - metabolism Liver - drug effects Liver regeneration Liver Regeneration - genetics Molecular and cellular biology Molecular genetics Oval cells Partial hepatectomy Rat liver Rats RNA, Messenger - analysis RNA, Messenger - metabolism Serial analysis of gene expression Stem Cells - metabolism Transcription, Genetic Transcriptome Up-Regulation |
| title | Transcriptome analysis of rat liver regeneration in a model of oval hepatic stem cells |
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