A novel N-acetyl-glucosamine lectin of Lonchocarpus araripensis attenuates acute cellular inflammation in mice

Objective and design This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis. Material/methods LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hem...

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Veröffentlicht in:	Inflammation research 2016-01, Vol.65 (1), p.43-52
Hauptverfasser:	Pires, Alana F., Rodrigues, Natália V. F. C., Soares, Pedro Marcos G., Ribeiro, Ronaldo de Albuquerque, Aragão, Karoline S., Marinho, Márcia M., da Silva, Mayara T. L., Cavada, Benildo S., Assreuy, Ana Maria S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - pharmacology Allergology Animals Anti-Inflammatory Agents - pharmacology Biomedical and Life Sciences Biomedicine Capillary Permeability - drug effects Dermatology Edema - chemically induced Edema - prevention & control Erythrocytes - drug effects Fabaceae - chemistry Hemagglutination - drug effects Immunology In Vitro Techniques Inflammation - pathology Inflammation - prevention & control Lectins - pharmacology Lonchocarpus Male Mice Neurology Original Research Paper Peritonitis - chemically induced Peritonitis - prevention & control Pharmacology/Toxicology Rabbits Rheumatology Seeds - chemistry
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Zusammenfassung:	Objective and design This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis. Material/methods LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hemagglutinating activity against rabbit erythrocytes. In vivo LAL was assessed for the anti-inflammatory activity via intravenous injection (i.v.) in Swiss mice (25–30 g; n = 6/group) in models of paw edema and peritonitis. Statistical analysis: ANOVA ( p
ISSN:	1023-3830 1420-908X
DOI:	10.1007/s00011-015-0889-7