Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26
Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide...
Gespeichert in:
| Veröffentlicht in: | Bioprocess and biosystems engineering 2016-01, Vol.39 (1), p.37-44 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 44 |
|---|---|
| container_issue | 1 |
| container_start_page | 37 |
| container_title | Bioprocess and biosystems engineering |
| container_volume | 39 |
| creator | Wei, Zhen-hua Liu, Lianliang Guo, Xiao-feng Li, Yan-jun Hou, Bao-chao Fan, Qiu-ling Wang, Kai-xiang Luo, Yingdi Zhong, Jian-Jiang |
| description | Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide (EPS) production rate. The mechanism was studied by analyzing the activities of enzymes related to polysaccharide biosynthesis. The results revealed that sucrose regulated the activities of phosphoglucomutase and phosphoglucose isomerase. When glucose and sucrose mixture was used as carbon source, biomass, polysaccharide and ganoderic acids (GAs) production was greatly enhanced. A sucrose fed-batch strategy was developed in 10-L bioreactor, and was scaled up to 300-L bioreactor. The biomass, EPS and IPS production was 25.5, 2.9 and 4.8 g/L, respectively, which was the highest biomass and IPS production in pilot scale. This study provides information for further understanding the regulation mechanism of Ganoderma polysaccharide biosynthesis. It demonstrates that sucrose fed-batch is a useful strategy for enhancing Ganoderma biomass, polysaccharide and GAs production. |
| doi_str_mv | 10.1007/s00449-015-1480-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1762369292</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3909956281</sourcerecordid><originalsourceid>FETCH-LOGICAL-c536t-c2cd5f8dcf0613923eb6fe3b1378d82966d0fa816c363ff62ef598e72857c8f03</originalsourceid><addsrcrecordid>eNqFkc1u1TAQhSMEoqXwAGzAEhsWTfFP_JNlVZWCVIlF6dpy7PG9rhL7YidS7wPw3vUlLUIsYGWP_J0znjlN85bgM4Kx_FQw7rq-xYS3pFO4vX_WHBNRKykwf_505z05al6VcocrqCh-2RxRwRmRXX_c_LxZbE4FkAfXDma2W1TmbGbY7BHErYkWHBpCmkwpp2iXxn0x1m5NDg5OkYkObUxMDnKwyNjgCtrl5BY7hxRRiNU2TxBn86tOHl2t9GTQuFR8mRA_o-J188KbscCbx_Okuf18-f3iS3v97errxfl1azkTc2upddwrZz0WhPWUwSA8sIEwqZyivRAOe6OIsEww7wUFz3sFkiourfKYnTQfV9_6yR8LlFlPoVgYRxMhLUUTKSgTPa3W_0c56zqOiaroh7_Qu7TkWAc5UFRJymRXKbJSh32XDF7vcphM3muC9SFOvcapa0r6EKe-r5p3j87LMIH7rXjKrwJ0BUp9ihvIf7T-h-v7VeRN0maTQ9G3NxQTgStKRMfYA5-ntIU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1752872374</pqid></control><display><type>article</type><title>Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Wei, Zhen-hua ; Liu, Lianliang ; Guo, Xiao-feng ; Li, Yan-jun ; Hou, Bao-chao ; Fan, Qiu-ling ; Wang, Kai-xiang ; Luo, Yingdi ; Zhong, Jian-Jiang</creator><creatorcontrib>Wei, Zhen-hua ; Liu, Lianliang ; Guo, Xiao-feng ; Li, Yan-jun ; Hou, Bao-chao ; Fan, Qiu-ling ; Wang, Kai-xiang ; Luo, Yingdi ; Zhong, Jian-Jiang</creatorcontrib><description>Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide (EPS) production rate. The mechanism was studied by analyzing the activities of enzymes related to polysaccharide biosynthesis. The results revealed that sucrose regulated the activities of phosphoglucomutase and phosphoglucose isomerase. When glucose and sucrose mixture was used as carbon source, biomass, polysaccharide and ganoderic acids (GAs) production was greatly enhanced. A sucrose fed-batch strategy was developed in 10-L bioreactor, and was scaled up to 300-L bioreactor. The biomass, EPS and IPS production was 25.5, 2.9 and 4.8 g/L, respectively, which was the highest biomass and IPS production in pilot scale. This study provides information for further understanding the regulation mechanism of Ganoderma polysaccharide biosynthesis. It demonstrates that sucrose fed-batch is a useful strategy for enhancing Ganoderma biomass, polysaccharide and GAs production.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-015-1480-x</identifier><identifier>PMID: 26531749</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; bioactive properties ; Bioengineering ; Biomass ; Bioreactors ; Biosynthesis ; Biotechnology ; Carbohydrates ; carbon ; Carbon sources ; Chemistry ; Chemistry and Materials Science ; Environmental Engineering/Biotechnology ; Fermentation ; Food Science ; Fungal Polysaccharides - biosynthesis ; ganoderic acid ; Ganoderma ; Ganoderma lucidum ; glucose ; glucose-6-phosphate isomerase ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Industrial production ; Mushrooms ; Oil and gas production ; Oriental traditional medicine ; Original Paper ; phosphoglucomutase ; polysaccharides ; Reishi - growth & development ; Sucrose ; Triterpenes - metabolism</subject><ispartof>Bioprocess and biosystems engineering, 2016-01, Vol.39 (1), p.37-44</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-c2cd5f8dcf0613923eb6fe3b1378d82966d0fa816c363ff62ef598e72857c8f03</citedby><cites>FETCH-LOGICAL-c536t-c2cd5f8dcf0613923eb6fe3b1378d82966d0fa816c363ff62ef598e72857c8f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-015-1480-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-015-1480-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26531749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Zhen-hua</creatorcontrib><creatorcontrib>Liu, Lianliang</creatorcontrib><creatorcontrib>Guo, Xiao-feng</creatorcontrib><creatorcontrib>Li, Yan-jun</creatorcontrib><creatorcontrib>Hou, Bao-chao</creatorcontrib><creatorcontrib>Fan, Qiu-ling</creatorcontrib><creatorcontrib>Wang, Kai-xiang</creatorcontrib><creatorcontrib>Luo, Yingdi</creatorcontrib><creatorcontrib>Zhong, Jian-Jiang</creatorcontrib><title>Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide (EPS) production rate. The mechanism was studied by analyzing the activities of enzymes related to polysaccharide biosynthesis. The results revealed that sucrose regulated the activities of phosphoglucomutase and phosphoglucose isomerase. When glucose and sucrose mixture was used as carbon source, biomass, polysaccharide and ganoderic acids (GAs) production was greatly enhanced. A sucrose fed-batch strategy was developed in 10-L bioreactor, and was scaled up to 300-L bioreactor. The biomass, EPS and IPS production was 25.5, 2.9 and 4.8 g/L, respectively, which was the highest biomass and IPS production in pilot scale. This study provides information for further understanding the regulation mechanism of Ganoderma polysaccharide biosynthesis. It demonstrates that sucrose fed-batch is a useful strategy for enhancing Ganoderma biomass, polysaccharide and GAs production.</description><subject>Acids</subject><subject>bioactive properties</subject><subject>Bioengineering</subject><subject>Biomass</subject><subject>Bioreactors</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Carbohydrates</subject><subject>carbon</subject><subject>Carbon sources</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Fermentation</subject><subject>Food Science</subject><subject>Fungal Polysaccharides - biosynthesis</subject><subject>ganoderic acid</subject><subject>Ganoderma</subject><subject>Ganoderma lucidum</subject><subject>glucose</subject><subject>glucose-6-phosphate isomerase</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Industrial production</subject><subject>Mushrooms</subject><subject>Oil and gas production</subject><subject>Oriental traditional medicine</subject><subject>Original Paper</subject><subject>phosphoglucomutase</subject><subject>polysaccharides</subject><subject>Reishi - growth & development</subject><subject>Sucrose</subject><subject>Triterpenes - metabolism</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1u1TAQhSMEoqXwAGzAEhsWTfFP_JNlVZWCVIlF6dpy7PG9rhL7YidS7wPw3vUlLUIsYGWP_J0znjlN85bgM4Kx_FQw7rq-xYS3pFO4vX_WHBNRKykwf_505z05al6VcocrqCh-2RxRwRmRXX_c_LxZbE4FkAfXDma2W1TmbGbY7BHErYkWHBpCmkwpp2iXxn0x1m5NDg5OkYkObUxMDnKwyNjgCtrl5BY7hxRRiNU2TxBn86tOHl2t9GTQuFR8mRA_o-J188KbscCbx_Okuf18-f3iS3v97errxfl1azkTc2upddwrZz0WhPWUwSA8sIEwqZyivRAOe6OIsEww7wUFz3sFkiourfKYnTQfV9_6yR8LlFlPoVgYRxMhLUUTKSgTPa3W_0c56zqOiaroh7_Qu7TkWAc5UFRJymRXKbJSh32XDF7vcphM3muC9SFOvcapa0r6EKe-r5p3j87LMIH7rXjKrwJ0BUp9ihvIf7T-h-v7VeRN0maTQ9G3NxQTgStKRMfYA5-ntIU</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Wei, Zhen-hua</creator><creator>Liu, Lianliang</creator><creator>Guo, Xiao-feng</creator><creator>Li, Yan-jun</creator><creator>Hou, Bao-chao</creator><creator>Fan, Qiu-ling</creator><creator>Wang, Kai-xiang</creator><creator>Luo, Yingdi</creator><creator>Zhong, Jian-Jiang</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20160101</creationdate><title>Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26</title><author>Wei, Zhen-hua ; Liu, Lianliang ; Guo, Xiao-feng ; Li, Yan-jun ; Hou, Bao-chao ; Fan, Qiu-ling ; Wang, Kai-xiang ; Luo, Yingdi ; Zhong, Jian-Jiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-c2cd5f8dcf0613923eb6fe3b1378d82966d0fa816c363ff62ef598e72857c8f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acids</topic><topic>bioactive properties</topic><topic>Bioengineering</topic><topic>Biomass</topic><topic>Bioreactors</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Carbohydrates</topic><topic>carbon</topic><topic>Carbon sources</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Fermentation</topic><topic>Food Science</topic><topic>Fungal Polysaccharides - biosynthesis</topic><topic>ganoderic acid</topic><topic>Ganoderma</topic><topic>Ganoderma lucidum</topic><topic>glucose</topic><topic>glucose-6-phosphate isomerase</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Industrial production</topic><topic>Mushrooms</topic><topic>Oil and gas production</topic><topic>Oriental traditional medicine</topic><topic>Original Paper</topic><topic>phosphoglucomutase</topic><topic>polysaccharides</topic><topic>Reishi - growth & development</topic><topic>Sucrose</topic><topic>Triterpenes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Zhen-hua</creatorcontrib><creatorcontrib>Liu, Lianliang</creatorcontrib><creatorcontrib>Guo, Xiao-feng</creatorcontrib><creatorcontrib>Li, Yan-jun</creatorcontrib><creatorcontrib>Hou, Bao-chao</creatorcontrib><creatorcontrib>Fan, Qiu-ling</creatorcontrib><creatorcontrib>Wang, Kai-xiang</creatorcontrib><creatorcontrib>Luo, Yingdi</creatorcontrib><creatorcontrib>Zhong, Jian-Jiang</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Zhen-hua</au><au>Liu, Lianliang</au><au>Guo, Xiao-feng</au><au>Li, Yan-jun</au><au>Hou, Bao-chao</au><au>Fan, Qiu-ling</au><au>Wang, Kai-xiang</au><au>Luo, Yingdi</au><au>Zhong, Jian-Jiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>39</volume><issue>1</issue><spage>37</spage><epage>44</epage><pages>37-44</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide (EPS) production rate. The mechanism was studied by analyzing the activities of enzymes related to polysaccharide biosynthesis. The results revealed that sucrose regulated the activities of phosphoglucomutase and phosphoglucose isomerase. When glucose and sucrose mixture was used as carbon source, biomass, polysaccharide and ganoderic acids (GAs) production was greatly enhanced. A sucrose fed-batch strategy was developed in 10-L bioreactor, and was scaled up to 300-L bioreactor. The biomass, EPS and IPS production was 25.5, 2.9 and 4.8 g/L, respectively, which was the highest biomass and IPS production in pilot scale. This study provides information for further understanding the regulation mechanism of Ganoderma polysaccharide biosynthesis. It demonstrates that sucrose fed-batch is a useful strategy for enhancing Ganoderma biomass, polysaccharide and GAs production.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26531749</pmid><doi>10.1007/s00449-015-1480-x</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1615-7591 |
| ispartof | Bioprocess and biosystems engineering, 2016-01, Vol.39 (1), p.37-44 |
| issn | 1615-7591 1615-7605 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1762369292 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Acids bioactive properties Bioengineering Biomass Bioreactors Biosynthesis Biotechnology Carbohydrates carbon Carbon sources Chemistry Chemistry and Materials Science Environmental Engineering/Biotechnology Fermentation Food Science Fungal Polysaccharides - biosynthesis ganoderic acid Ganoderma Ganoderma lucidum glucose glucose-6-phosphate isomerase Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Industrial production Mushrooms Oil and gas production Oriental traditional medicine Original Paper phosphoglucomutase polysaccharides Reishi - growth & development Sucrose Triterpenes - metabolism |
| title | Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T22%3A12%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sucrose%20fed-batch%20strategy%20enhanced%20biomass,%20polysaccharide,%20and%20ganoderic%20acids%20production%20in%20fermentation%20of%20Ganoderma%20lucidum%205.26&rft.jtitle=Bioprocess%20and%20biosystems%20engineering&rft.au=Wei,%20Zhen-hua&rft.date=2016-01-01&rft.volume=39&rft.issue=1&rft.spage=37&rft.epage=44&rft.pages=37-44&rft.issn=1615-7591&rft.eissn=1615-7605&rft_id=info:doi/10.1007/s00449-015-1480-x&rft_dat=%3Cproquest_cross%3E3909956281%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1752872374&rft_id=info:pmid/26531749&rfr_iscdi=true |