Expression and Substrate Range of Streptomyces Vanillate Demethylase

Vanillate is converted to protocatechuate by an O-demethylase consisting of VanA and VanB in Streptomyces sp. NL15-2K. In this study, vanillate demethylase from this strain was functionally expressed in Escherichia coli, and its substrate range for vanillate analogs was determined by an in vivo assa...

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Veröffentlicht in:	Biological & pharmaceutical bulletin 2014/09/01, Vol.37(9), pp.1564-1568
Hauptverfasser:	Nishimura, Motohiro, Nishimura, Yoshio, Abe, Chinatsu, Kohhata, Mayuko
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics expression Oxidoreductases, O-Demethylating - genetics Oxidoreductases, O-Demethylating - metabolism Plasmids Streptomyces Streptomyces - enzymology Streptomyces - genetics substrate range vanillate demethylase Vanillic Acid - analogs & derivatives Vanillic Acid - metabolism
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