Monitoring Tumor Response to Anticancer Drugs Using Stable Three-Dimensional Culture in a Recyclable Microfluidic Platform
The development and application of miniaturized platforms with the capability for microscale and dynamic control of biomimetic and high-throughput three-dimensional (3D) culture plays a crucial role in biological research. In this study, pneumatic microstructure-based microfluidics was used to syste...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2015-10, Vol.87 (19), p.9752-9760 |
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| creator | Liu, Wenming Xu, Juan Li, Tianbao Zhao, Lei Ma, Chao Shen, Shaofei Wang, Jinyi |
| description | The development and application of miniaturized platforms with the capability for microscale and dynamic control of biomimetic and high-throughput three-dimensional (3D) culture plays a crucial role in biological research. In this study, pneumatic microstructure-based microfluidics was used to systematically demonstrate 3D tumor culture under various culture conditions. We also demonstrated the reusability of the fabrication-optimized pneumatic device for high-throughput cell manipulation and 3D tumor culture. This microfluidic system provides remarkably long-term (over 1 month) and cyclic stability. Furthermore, temporal and high-throughput monitoring of tumor response to evaluate the therapeutic efficacy of different chemotherapies, was achieved based on the robust culture. This advancement in microfluidics has potential applications in the fields of tissue engineering, tumor biology, and clinical medicine; it also provides new insight into the construction of high-performance and recyclable microplatforms for cancer research. |
| doi_str_mv | 10.1021/acs.analchem.5b01915 |
| format | Article |
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In this study, pneumatic microstructure-based microfluidics was used to systematically demonstrate 3D tumor culture under various culture conditions. We also demonstrated the reusability of the fabrication-optimized pneumatic device for high-throughput cell manipulation and 3D tumor culture. This microfluidic system provides remarkably long-term (over 1 month) and cyclic stability. Furthermore, temporal and high-throughput monitoring of tumor response to evaluate the therapeutic efficacy of different chemotherapies, was achieved based on the robust culture. 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Chem</addtitle><description>The development and application of miniaturized platforms with the capability for microscale and dynamic control of biomimetic and high-throughput three-dimensional (3D) culture plays a crucial role in biological research. In this study, pneumatic microstructure-based microfluidics was used to systematically demonstrate 3D tumor culture under various culture conditions. We also demonstrated the reusability of the fabrication-optimized pneumatic device for high-throughput cell manipulation and 3D tumor culture. This microfluidic system provides remarkably long-term (over 1 month) and cyclic stability. Furthermore, temporal and high-throughput monitoring of tumor response to evaluate the therapeutic efficacy of different chemotherapies, was achieved based on the robust culture. 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Xu, Juan ; Li, Tianbao ; Zhao, Lei ; Ma, Chao ; Shen, Shaofei ; Wang, Jinyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a508t-3899b9428b45bf51088800a3dfc2ff0e77c056e55aef7f204a4dadc6825f73543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>3-D graphics</topic><topic>Analytical chemistry</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Biomimetics</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Cell Line, Tumor</topic><topic>Chemotherapy</topic><topic>Culture</topic><topic>Drug Screening Assays, Antitumor - instrumentation</topic><topic>Equipment Design</topic><topic>Equipment Reuse</topic><topic>High-Throughput Screening Assays - instrumentation</topic><topic>Humans</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidics</topic><topic>Microstructure</topic><topic>Monitoring</topic><topic>Neoplasms - drug therapy</topic><topic>Neoplasms - metabolism</topic><topic>Neoplasms - pathology</topic><topic>Oncology</topic><topic>Platforms</topic><topic>Recycling</topic><topic>Signal Transduction</topic><topic>Three dimensional</topic><topic>Tissue engineering</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Wenming</creatorcontrib><creatorcontrib>Xu, Juan</creatorcontrib><creatorcontrib>Li, Tianbao</creatorcontrib><creatorcontrib>Zhao, Lei</creatorcontrib><creatorcontrib>Ma, Chao</creatorcontrib><creatorcontrib>Shen, Shaofei</creatorcontrib><creatorcontrib>Wang, Jinyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Wenming</au><au>Xu, Juan</au><au>Li, Tianbao</au><au>Zhao, Lei</au><au>Ma, Chao</au><au>Shen, Shaofei</au><au>Wang, Jinyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring Tumor Response to Anticancer Drugs Using Stable Three-Dimensional Culture in a Recyclable Microfluidic Platform</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2015-10-06</date><risdate>2015</risdate><volume>87</volume><issue>19</issue><spage>9752</spage><epage>9760</epage><pages>9752-9760</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>The development and application of miniaturized platforms with the capability for microscale and dynamic control of biomimetic and high-throughput three-dimensional (3D) culture plays a crucial role in biological research. In this study, pneumatic microstructure-based microfluidics was used to systematically demonstrate 3D tumor culture under various culture conditions. We also demonstrated the reusability of the fabrication-optimized pneumatic device for high-throughput cell manipulation and 3D tumor culture. This microfluidic system provides remarkably long-term (over 1 month) and cyclic stability. 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| ispartof | Analytical chemistry (Washington), 2015-10, Vol.87 (19), p.9752-9760 |
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| subjects | 3-D graphics Analytical chemistry Antineoplastic Agents - pharmacology Apoptosis - drug effects Biomimetics Cell Culture Techniques - instrumentation Cell Line, Tumor Chemotherapy Culture Drug Screening Assays, Antitumor - instrumentation Equipment Design Equipment Reuse High-Throughput Screening Assays - instrumentation Humans Microfluidic Analytical Techniques - instrumentation Microfluidics Microstructure Monitoring Neoplasms - drug therapy Neoplasms - metabolism Neoplasms - pathology Oncology Platforms Recycling Signal Transduction Three dimensional Tissue engineering Tumors |
| title | Monitoring Tumor Response to Anticancer Drugs Using Stable Three-Dimensional Culture in a Recyclable Microfluidic Platform |
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