The Nanoscale Geometrical Maturation of Focal Adhesions Controls Stem Cell Differentiation and Mechanotransduction
We show that the nanoscale adhesion geometry controls the spreading and differentiation of epidermal stem cells. We find that cells respond to such hard nanopatterns similarly to their behavior on soft hydrogels. Cellular responses were seen to stem from local changes in diffusion dynamics of the ad...
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| Veröffentlicht in: | Nano letters 2014-07, Vol.14 (7), p.3945-3952 |
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| container_title | Nano letters |
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| creator | Gautrot, Julien E Malmström, Jenny Sundh, Maria Margadant, Coert Sonnenberg, Arnoud Sutherland, Duncan S |
| description | We show that the nanoscale adhesion geometry controls the spreading and differentiation of epidermal stem cells. We find that cells respond to such hard nanopatterns similarly to their behavior on soft hydrogels. Cellular responses were seen to stem from local changes in diffusion dynamics of the adapter protein vinculin and associated impaired mechanotransduction rather than impaired recruitment of proteins involved in focal adhesion formation. |
| doi_str_mv | 10.1021/nl501248y |
| format | Article |
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We find that cells respond to such hard nanopatterns similarly to their behavior on soft hydrogels. 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We find that cells respond to such hard nanopatterns similarly to their behavior on soft hydrogels. Cellular responses were seen to stem from local changes in diffusion dynamics of the adapter protein vinculin and associated impaired mechanotransduction rather than impaired recruitment of proteins involved in focal adhesion formation.</description><subject>Adhesion</subject><subject>Biocompatible Materials - chemistry</subject><subject>Cell Adhesion</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Condensed matter: structure, mechanical and thermal properties</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>Differentiation</subject><subject>Diffusion in nanoscale solids</subject><subject>Diffusion in solids</subject><subject>Exact sciences and technology</subject><subject>Focal Adhesions - metabolism</subject><subject>Humans</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - metabolism</subject><subject>Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties</subject><subject>Materials science</subject><subject>Mechanical and acoustical properties of condensed matter</subject><subject>Mechanical properties of nanoscale materials</subject><subject>Mechanotransduction, Cellular</subject><subject>Methods of nanofabrication</subject><subject>Nanoscale pattern formation</subject><subject>Nanostructure</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - ultrastructure</subject><subject>Phosphorylation</subject><subject>Physics</subject><subject>Proteins</subject><subject>Recruitment</subject><subject>Spreading</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties)</subject><subject>Transport properties of condensed matter (nonelectronic)</subject><subject>Vinculin</subject><subject>Vinculin - metabolism</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPxCAUhYnR-F74BwwbE12M8mqhSzPqaOJj4bhuKL1kalpQoAv_vUxmHDcmri4cPs6FcxE6oeSSEkavXF8QyoT62kL7tOBkUlYV296sldhDBzG-E0IqXpBdtJdZoSqp9lGYLwA_a-ej0T3gGfgBUujyBj_pNAadOu-wt_jOL7XrdgExKxFPvUvB9xG_JhjwFPoe33TWQgCXutUt7Vr8BGaR3VPQLrajWepHaMfqPsLxuh6it7vb-fR-8vgye5heP060kFWaKGpZw6URRrYNL4UGVtp8pA0xuUhNWygt5C82LVMl8JIIInlRMMokrRp-iM5Xvh_Bf44QUz100eSHagd-jDWVJSNFwbn6Hy2EkFwyJjJ6sUJN8DEGsPVH6AYdvmpK6uU06s00Mnu6th2bAdoN-RN_Bs7WgF7mb3NKpou_nCpVRXPnDadNrN_9GFwO7o-G30wEnmo</recordid><startdate>20140709</startdate><enddate>20140709</enddate><creator>Gautrot, Julien E</creator><creator>Malmström, Jenny</creator><creator>Sundh, Maria</creator><creator>Margadant, Coert</creator><creator>Sonnenberg, Arnoud</creator><creator>Sutherland, Duncan S</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20140709</creationdate><title>The Nanoscale Geometrical Maturation of Focal Adhesions Controls Stem Cell Differentiation and Mechanotransduction</title><author>Gautrot, Julien E ; Malmström, Jenny ; Sundh, Maria ; Margadant, Coert ; Sonnenberg, Arnoud ; Sutherland, Duncan S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a479t-81f2b37c4c7db364ae26fa47ac0ca477a1de6fe530bd286e360407355212719b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adhesion</topic><topic>Biocompatible Materials - chemistry</topic><topic>Cell Adhesion</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Condensed matter: structure, mechanical and thermal properties</topic><topic>Cross-disciplinary physics: materials science; rheology</topic><topic>Differentiation</topic><topic>Diffusion in nanoscale solids</topic><topic>Diffusion in solids</topic><topic>Exact sciences and technology</topic><topic>Focal Adhesions - metabolism</topic><topic>Humans</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - metabolism</topic><topic>Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties</topic><topic>Materials science</topic><topic>Mechanical and acoustical properties of condensed matter</topic><topic>Mechanical properties of nanoscale materials</topic><topic>Mechanotransduction, Cellular</topic><topic>Methods of nanofabrication</topic><topic>Nanoscale pattern formation</topic><topic>Nanostructure</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - ultrastructure</topic><topic>Phosphorylation</topic><topic>Physics</topic><topic>Proteins</topic><topic>Recruitment</topic><topic>Spreading</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties)</topic><topic>Transport properties of condensed matter (nonelectronic)</topic><topic>Vinculin</topic><topic>Vinculin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gautrot, Julien E</creatorcontrib><creatorcontrib>Malmström, Jenny</creatorcontrib><creatorcontrib>Sundh, Maria</creatorcontrib><creatorcontrib>Margadant, Coert</creatorcontrib><creatorcontrib>Sonnenberg, Arnoud</creatorcontrib><creatorcontrib>Sutherland, Duncan S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gautrot, Julien E</au><au>Malmström, Jenny</au><au>Sundh, Maria</au><au>Margadant, Coert</au><au>Sonnenberg, Arnoud</au><au>Sutherland, Duncan S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Nanoscale Geometrical Maturation of Focal Adhesions Controls Stem Cell Differentiation and Mechanotransduction</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2014-07-09</date><risdate>2014</risdate><volume>14</volume><issue>7</issue><spage>3945</spage><epage>3952</epage><pages>3945-3952</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>We show that the nanoscale adhesion geometry controls the spreading and differentiation of epidermal stem cells. 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| subjects | Adhesion Biocompatible Materials - chemistry Cell Adhesion Cell Differentiation Cells, Cultured Condensed matter: structure, mechanical and thermal properties Cross-disciplinary physics: materials science rheology Differentiation Diffusion in nanoscale solids Diffusion in solids Exact sciences and technology Focal Adhesions - metabolism Humans Keratinocytes - cytology Keratinocytes - metabolism Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties Materials science Mechanical and acoustical properties of condensed matter Mechanical properties of nanoscale materials Mechanotransduction, Cellular Methods of nanofabrication Nanoscale pattern formation Nanostructure Nanostructures - chemistry Nanostructures - ultrastructure Phosphorylation Physics Proteins Recruitment Spreading Stem cells Stem Cells - cytology Stem Cells - metabolism Surfaces and interfaces thin films and whiskers (structure and nonelectronic properties) Transport properties of condensed matter (nonelectronic) Vinculin Vinculin - metabolism |
| title | The Nanoscale Geometrical Maturation of Focal Adhesions Controls Stem Cell Differentiation and Mechanotransduction |
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