Photoswitchable Oligonucleotide-Modified Gold Nanoparticles: Controlling Hybridization Stringency with Photon Dose
We describe a new class of stimulus-responsive DNA-functionalized gold nanoparticles that incorporate azobenzene-modified oligonucleotides. Beyond the classic directed assembly and sensing behaviors associated with oligonucleotide-modified nanoparticles, these particles also exhibit reversible photo...
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| Veröffentlicht in: | Nano letters 2012-05, Vol.12 (5), p.2530-2536 |
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| container_title | Nano letters |
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| creator | Yan, Yunqi Chen, Jennifer I. L Ginger, David S |
| description | We describe a new class of stimulus-responsive DNA-functionalized gold nanoparticles that incorporate azobenzene-modified oligonucleotides. Beyond the classic directed assembly and sensing behaviors associated with oligonucleotide-modified nanoparticles, these particles also exhibit reversible photoswitching of their assembly behavior. Exposure to UV light induces a trans–cis isomerization of the azobenzene which destabilizes the DNA duplex, resulting in dissociation of the nanoparticle assemblies. The isomerization is reversible upon exposure to blue light, resulting in rehybridization and reassembly of the DNA-linked nanoparticle clusters. We show that perfectly complementary and partially mismatched strands exhibit clearly distinguishable photoinduced melting properties, and we demonstrate that photon dose can thus be used in place of temperature or ionic strength to control hybridization stringency with the ability to discriminate single-base mismatches. |
| doi_str_mv | 10.1021/nl300739n |
| format | Article |
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We show that perfectly complementary and partially mismatched strands exhibit clearly distinguishable photoinduced melting properties, and we demonstrate that photon dose can thus be used in place of temperature or ionic strength to control hybridization stringency with the ability to discriminate single-base mismatches.</description><subject>Assembly</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>Exact sciences and technology</subject><subject>Exposure</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Isomerization</subject><subject>Materials science</subject><subject>Melting</subject><subject>Metal Nanoparticles</subject><subject>Nanocrystalline materials</subject><subject>Nanoparticles</subject><subject>Nanoscale materials and structures: fabrication and characterization</subject><subject>Nucleic Acid Conformation</subject><subject>Oligonucleotides</subject><subject>Oligonucleotides - chemistry</subject><subject>Photons</subject><subject>Physics</subject><subject>Strands</subject><subject>Ultraviolet Rays</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UFvFCEYBmBiNLZWD_4Bw8VED6MfMAzgzazamlRrop4nDDBdGha2wMSsv15q1-3FpCcIPHk_wovQcwJvCFDyNgYGIJiKD9Ax4Qy6QSn68LCX_RF6UsoVACjG4TE6orRXTKnhGOVv61RT-eWrWespOHwR_GWKiwkuVW9d9yVZP3tn8WkKFn_VMW11rr7dl3d4lWLNKQQfL_HZbsre-t-6-hTx95rboYtmh1v2Gv8dE_GHVNxT9GjWobhn-_UE_fz08cfqrDu_OP28en_e6Z6Q2pn2QmqlMdaoGTjvoQdqJ6U1tVpJJ5SeBjEwK5VTMHDppklTkETMwupJshP06jZ3m9P14kodN74YF4KOLi1lJGKgwKmU6n7KWS-p4kzcT4FQCmKAm9TXt9TkVEp287jNfqPzrqHxprjxUFyzL_axy7Rx9iD_NdXAyz3QxegwZx2NL3eOS9GC4M5pU8artOTY_vg_A_8A-U6tnQ</recordid><startdate>20120509</startdate><enddate>20120509</enddate><creator>Yan, Yunqi</creator><creator>Chen, Jennifer I. 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L</au><au>Ginger, David S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photoswitchable Oligonucleotide-Modified Gold Nanoparticles: Controlling Hybridization Stringency with Photon Dose</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2012-05-09</date><risdate>2012</risdate><volume>12</volume><issue>5</issue><spage>2530</spage><epage>2536</epage><pages>2530-2536</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>We describe a new class of stimulus-responsive DNA-functionalized gold nanoparticles that incorporate azobenzene-modified oligonucleotides. Beyond the classic directed assembly and sensing behaviors associated with oligonucleotide-modified nanoparticles, these particles also exhibit reversible photoswitching of their assembly behavior. Exposure to UV light induces a trans–cis isomerization of the azobenzene which destabilizes the DNA duplex, resulting in dissociation of the nanoparticle assemblies. 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| ispartof | Nano letters, 2012-05, Vol.12 (5), p.2530-2536 |
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| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Assembly Cross-disciplinary physics: materials science rheology Exact sciences and technology Exposure Gold Gold - chemistry Isomerization Materials science Melting Metal Nanoparticles Nanocrystalline materials Nanoparticles Nanoscale materials and structures: fabrication and characterization Nucleic Acid Conformation Oligonucleotides Oligonucleotides - chemistry Photons Physics Strands Ultraviolet Rays |
| title | Photoswitchable Oligonucleotide-Modified Gold Nanoparticles: Controlling Hybridization Stringency with Photon Dose |
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