Tuning Nanoparticle Uptake: Live-Cell Imaging Reveals Two Distinct Endocytosis Mechanisms Mediated by Natural and Artificial EGFR Targeting Ligand
Therapeutic nanoparticles can be directed to cancer cells by incorporating selective targeting ligands. Here, we investigate the epidermal growth factor receptor (EGFR)-mediated endocytosis of gene carriers (polyplexes) either targeted with natural EGF or GE11, a short synthetic EGFR-binding peptide...
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| Veröffentlicht in: | Nano letters 2012-07, Vol.12 (7), p.3417-3423 |
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| container_end_page | 3423 |
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| container_issue | 7 |
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| container_title | Nano letters |
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| creator | Mickler, Frauke M Möckl, Leonhard Ruthardt, Nadia Ogris, Manfred Wagner, Ernst Bräuchle, Christoph |
| description | Therapeutic nanoparticles can be directed to cancer cells by incorporating selective targeting ligands. Here, we investigate the epidermal growth factor receptor (EGFR)-mediated endocytosis of gene carriers (polyplexes) either targeted with natural EGF or GE11, a short synthetic EGFR-binding peptide. Highly sensitive live-cell fluorescence microcopy with single particle resolution unraveled the existence of two different uptake mechanisms; EGF triggers accelerated nanoparticle endocytosis due to its dual active role in receptor binding and signaling activation. For GE11, an alternative EGFR signaling independent, actin-driven pathway is presented. |
| doi_str_mv | 10.1021/nl300395q |
| format | Article |
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| ispartof | Nano letters, 2012-07, Vol.12 (7), p.3417-3423 |
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| source | MEDLINE; ACS Publications |
| subjects | Actins - metabolism Binding Cell Line, Tumor Cross-disciplinary physics: materials science rheology Drug Carriers - chemistry Drug Carriers - metabolism Endocytosis Exact sciences and technology Gene Transfer Techniques Genes Growth factors Humans Ligands Materials science Microscopy, Fluorescence Nanocrystalline materials Nanoparticles Nanoparticles - chemistry Nanoscale materials and structures: fabrication and characterization Nanostructure Peptides - chemistry Peptides - metabolism Physics Receptor, Epidermal Growth Factor - chemistry Receptor, Epidermal Growth Factor - metabolism Receptors Signal Transduction Tuning Uptakes |
| title | Tuning Nanoparticle Uptake: Live-Cell Imaging Reveals Two Distinct Endocytosis Mechanisms Mediated by Natural and Artificial EGFR Targeting Ligand |
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