Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin
Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a...
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| Veröffentlicht in: | ACS combinatorial science 2013-09, Vol.15 (9), p.452-457 |
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| container_end_page | 457 |
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| container_issue | 9 |
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| container_title | ACS combinatorial science |
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| creator | Er, Jun Cheng Vendrell, Marc Tang, Mui Kee Zhai, Duanting Chang, Young-Tae |
| description | Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites. |
| doi_str_mv | 10.1021/co400060b |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 2156-8952 |
| ispartof | ACS combinatorial science, 2013-09, Vol.15 (9), p.452-457 |
| issn | 2156-8952 2156-8944 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Binding Binding Sites Combinatorial analysis Drugs Dyes Emission Fluorescence Fluorescent Dyes - chemistry High-Throughput Screening Assays Humans Incompatibility Mapping Models, Molecular Molecular Structure Multiplexing Pharmaceutical Preparations - chemistry Serum Albumin - chemistry |
| title | Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin |
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