Development of arm specific RAPD markers for rye chromosome 2R in wheat
Rye (Secale cereale L.) chromosome 2R carries several genes for resistance to biotic and abiotic stresses, which could be used in wheat (Triticum aestivum L.) improvement. To make these genes accessible in breeding, translocations of 2R to wheat chromosome 2B have been generated. To compensate for t...
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| Veröffentlicht in: | Crop science 1999-11, Vol.39 (6), p.1702-1706 |
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| creator | Brunell, M.S Lukaszewski, A.J Whitkus, R |
| description | Rye (Secale cereale L.) chromosome 2R carries several genes for resistance to biotic and abiotic stresses, which could be used in wheat (Triticum aestivum L.) improvement. To make these genes accessible in breeding, translocations of 2R to wheat chromosome 2B have been generated. To compensate for the lack of clearly identifiable cytological markers on chromosome 2R, random amplified polymorphic DNAs (RAPDs) were employed as markers. Wheat lines homozygous for centric translocations 2RS.2BL and 2BS.2RL were screened with 489 primers, resulting in 65 clear and reproducible RAPD polymorphisms. To assign the polymorphisms to individual arms of chromosomes 2R and 2B, the arms of the translocations were separated by centric misdivision and the resulting progeny with desired chromosome constructs were rescreened. As a result, 17 markers were assigned to chromosome arm 2RS, 15 to 2RL, and the remaining 33 most likely located on 2B. Preliminary mapping studies (not shown here) have indicated that some of these markers are evenly distributed along the chromosome arms. These markers should be useful for the localization of translocation breakpoints in recombinant wheat-rye chromosomes. |
| doi_str_mv | 10.2135/cropsci1999.3961702x |
| format | Article |
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To make these genes accessible in breeding, translocations of 2R to wheat chromosome 2B have been generated. To compensate for the lack of clearly identifiable cytological markers on chromosome 2R, random amplified polymorphic DNAs (RAPDs) were employed as markers. Wheat lines homozygous for centric translocations 2RS.2BL and 2BS.2RL were screened with 489 primers, resulting in 65 clear and reproducible RAPD polymorphisms. To assign the polymorphisms to individual arms of chromosomes 2R and 2B, the arms of the translocations were separated by centric misdivision and the resulting progeny with desired chromosome constructs were rescreened. As a result, 17 markers were assigned to chromosome arm 2RS, 15 to 2RL, and the remaining 33 most likely located on 2B. Preliminary mapping studies (not shown here) have indicated that some of these markers are evenly distributed along the chromosome arms. These markers should be useful for the localization of translocation breakpoints in recombinant wheat-rye chromosomes.</description><identifier>ISSN: 0011-183X</identifier><identifier>EISSN: 1435-0653</identifier><identifier>DOI: 10.2135/cropsci1999.3961702x</identifier><identifier>CODEN: CRPSAY</identifier><language>eng</language><publisher>Madison: Crop Science Society of America</publisher><subject>Agronomy. Soil science and plant productions ; Analysis ; backcrossing ; Biological and medical sciences ; Biotechnology industry ; Breeding ; cell biology ; chromosome 2B ; chromosome 2R ; chromosome mapping ; chromosome translocation ; Chromosomes ; Classical and quantitative genetics. Population genetics. Molecular genetics ; cytogenetic analysis ; Cytogenetics ; Diseases and pests ; Fundamental and applied biological sciences. Psychology ; gene location ; Generalities. Genetics. Plant material ; Genes ; Genetic engineering ; genetic markers ; genetic polymorphism ; Genetics and breeding of economic plants ; Grain ; homozygosity ; intergeneric hybridization ; Molecular genetics ; Plants ; random amplified polymorphic DNA technique ; Rye ; Secale cereale ; triticale ; Triticum aestivum ; Wheat</subject><ispartof>Crop science, 1999-11, Vol.39 (6), p.1702-1706</ispartof><rights>Crop Science Society of America</rights><rights>2000 INIST-CNRS</rights><rights>COPYRIGHT 1999 Crop Science Society of America</rights><rights>Copyright American Society of Agronomy Nov/Dec 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c567X-bcf907a0591e42aabcc539aff543300288566ca758a14c96915c5cbe2947a8d83</citedby><cites>FETCH-LOGICAL-c567X-bcf907a0591e42aabcc539aff543300288566ca758a14c96915c5cbe2947a8d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.2135%2Fcropsci1999.3961702x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.2135%2Fcropsci1999.3961702x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,1417,23930,23931,25140,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1285173$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Brunell, M.S</creatorcontrib><creatorcontrib>Lukaszewski, A.J</creatorcontrib><creatorcontrib>Whitkus, R</creatorcontrib><title>Development of arm specific RAPD markers for rye chromosome 2R in wheat</title><title>Crop science</title><description>Rye (Secale cereale L.) chromosome 2R carries several genes for resistance to biotic and abiotic stresses, which could be used in wheat (Triticum aestivum L.) improvement. To make these genes accessible in breeding, translocations of 2R to wheat chromosome 2B have been generated. To compensate for the lack of clearly identifiable cytological markers on chromosome 2R, random amplified polymorphic DNAs (RAPDs) were employed as markers. Wheat lines homozygous for centric translocations 2RS.2BL and 2BS.2RL were screened with 489 primers, resulting in 65 clear and reproducible RAPD polymorphisms. To assign the polymorphisms to individual arms of chromosomes 2R and 2B, the arms of the translocations were separated by centric misdivision and the resulting progeny with desired chromosome constructs were rescreened. As a result, 17 markers were assigned to chromosome arm 2RS, 15 to 2RL, and the remaining 33 most likely located on 2B. Preliminary mapping studies (not shown here) have indicated that some of these markers are evenly distributed along the chromosome arms. These markers should be useful for the localization of translocation breakpoints in recombinant wheat-rye chromosomes.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Analysis</subject><subject>backcrossing</subject><subject>Biological and medical sciences</subject><subject>Biotechnology industry</subject><subject>Breeding</subject><subject>cell biology</subject><subject>chromosome 2B</subject><subject>chromosome 2R</subject><subject>chromosome mapping</subject><subject>chromosome translocation</subject><subject>Chromosomes</subject><subject>Classical and quantitative genetics. Population genetics. Molecular genetics</subject><subject>cytogenetic analysis</subject><subject>Cytogenetics</subject><subject>Diseases and pests</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene location</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>genetic markers</subject><subject>genetic polymorphism</subject><subject>Genetics and breeding of economic plants</subject><subject>Grain</subject><subject>homozygosity</subject><subject>intergeneric hybridization</subject><subject>Molecular genetics</subject><subject>Plants</subject><subject>random amplified polymorphic DNA technique</subject><subject>Rye</subject><subject>Secale cereale</subject><subject>triticale</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>0011-183X</issn><issn>1435-0653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqNkd9rFDEQxxdR8Kz-B4JBxCe35sdms3k8tloPCi13Fu4tzKWTa-ruZk3urPffm7InivRB5mFg5jPfGeZbFK8ZPeVMyI82hjFZz7TWp0LXTFH-80kxY5WQJa2leFrMKGWsZI1YPy9epHRHKVVayVlxfoY_sAtjj8OOBEcg9iSNaL3zliznV2ekh_gNYyIuRBIPSOxtDH1IoUfCl8QP5P4WYfeyeOagS_jqmE-K68-fvrZfyovL80U7vyitrNW63FinqQIqNcOKA2yslUKDc7ISglLeNLKuLSjZAKusrjWTVtoNcl0paG4acVK8n3THGL7vMe1M75PFroMBwz4Zpur8kYpl8O0_4F3YxyHfZjjjNReNohn6MEFb6ND4wYVdBLvFASN0YUDnc3leU1pVUqqMl4_gOW6w9_Yxvpr4bFBKEZ0Zo8__PBhGzYNz5i_nzG_n8ti74-mQLHQuwmB9-jPLG8mUyNhiwu7z1sN_SZt21fJ2eXm1ahcPjWN9nbXeTFoOgoFtzOuuV5wyQbmWtKZS_AKCJLf5</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Brunell, M.S</creator><creator>Lukaszewski, A.J</creator><creator>Whitkus, R</creator><general>Crop Science Society of America</general><general>American Society of Agronomy</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199911</creationdate><title>Development of arm specific RAPD markers for rye chromosome 2R in wheat</title><author>Brunell, M.S ; Lukaszewski, A.J ; Whitkus, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c567X-bcf907a0591e42aabcc539aff543300288566ca758a14c96915c5cbe2947a8d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Analysis</topic><topic>backcrossing</topic><topic>Biological and medical sciences</topic><topic>Biotechnology industry</topic><topic>Breeding</topic><topic>cell biology</topic><topic>chromosome 2B</topic><topic>chromosome 2R</topic><topic>chromosome mapping</topic><topic>chromosome translocation</topic><topic>Chromosomes</topic><topic>Classical and quantitative genetics. Population genetics. Molecular genetics</topic><topic>cytogenetic analysis</topic><topic>Cytogenetics</topic><topic>Diseases and pests</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene location</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>genetic markers</topic><topic>genetic polymorphism</topic><topic>Genetics and breeding of economic plants</topic><topic>Grain</topic><topic>homozygosity</topic><topic>intergeneric hybridization</topic><topic>Molecular genetics</topic><topic>Plants</topic><topic>random amplified polymorphic DNA technique</topic><topic>Rye</topic><topic>Secale cereale</topic><topic>triticale</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brunell, M.S</creatorcontrib><creatorcontrib>Lukaszewski, A.J</creatorcontrib><creatorcontrib>Whitkus, R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Crop science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brunell, M.S</au><au>Lukaszewski, A.J</au><au>Whitkus, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of arm specific RAPD markers for rye chromosome 2R in wheat</atitle><jtitle>Crop science</jtitle><date>1999-11</date><risdate>1999</risdate><volume>39</volume><issue>6</issue><spage>1702</spage><epage>1706</epage><pages>1702-1706</pages><issn>0011-183X</issn><eissn>1435-0653</eissn><coden>CRPSAY</coden><abstract>Rye (Secale cereale L.) chromosome 2R carries several genes for resistance to biotic and abiotic stresses, which could be used in wheat (Triticum aestivum L.) improvement. To make these genes accessible in breeding, translocations of 2R to wheat chromosome 2B have been generated. To compensate for the lack of clearly identifiable cytological markers on chromosome 2R, random amplified polymorphic DNAs (RAPDs) were employed as markers. Wheat lines homozygous for centric translocations 2RS.2BL and 2BS.2RL were screened with 489 primers, resulting in 65 clear and reproducible RAPD polymorphisms. To assign the polymorphisms to individual arms of chromosomes 2R and 2B, the arms of the translocations were separated by centric misdivision and the resulting progeny with desired chromosome constructs were rescreened. As a result, 17 markers were assigned to chromosome arm 2RS, 15 to 2RL, and the remaining 33 most likely located on 2B. Preliminary mapping studies (not shown here) have indicated that some of these markers are evenly distributed along the chromosome arms. These markers should be useful for the localization of translocation breakpoints in recombinant wheat-rye chromosomes.</abstract><cop>Madison</cop><pub>Crop Science Society of America</pub><doi>10.2135/cropsci1999.3961702x</doi><tpages>5</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Analysis backcrossing Biological and medical sciences Biotechnology industry Breeding cell biology chromosome 2B chromosome 2R chromosome mapping chromosome translocation Chromosomes Classical and quantitative genetics. Population genetics. Molecular genetics cytogenetic analysis Cytogenetics Diseases and pests Fundamental and applied biological sciences. Psychology gene location Generalities. Genetics. Plant material Genes Genetic engineering genetic markers genetic polymorphism Genetics and breeding of economic plants Grain homozygosity intergeneric hybridization Molecular genetics Plants random amplified polymorphic DNA technique Rye Secale cereale triticale Triticum aestivum Wheat |
| title | Development of arm specific RAPD markers for rye chromosome 2R in wheat |
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