Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs

OBJECTIVE: To investigate the effect of 66% Manuka honey gel on the concentrations of transforming growth factor (TGF)‐β1 and TGF‐β3, bacterial counts and histomorphology during healing of contaminated equine distal limb wounds. METHODS: In this experimental study of 10 Standardbred horses, five ful...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Australian veterinary journal 2016, Vol.94 (1-2), p.27-34
Hauptverfasser: Bischofberger, AS, Dart, CM, Horadagoda, N, Perkins, NR, Jeffcott, LB, Little, CB, Dart, AJ
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 34
container_issue 1-2
container_start_page 27
container_title Australian veterinary journal
container_volume 94
creator Bischofberger, AS
Dart, CM
Horadagoda, N
Perkins, NR
Jeffcott, LB
Little, CB
Dart, AJ
description OBJECTIVE: To investigate the effect of 66% Manuka honey gel on the concentrations of transforming growth factor (TGF)‐β1 and TGF‐β3, bacterial counts and histomorphology during healing of contaminated equine distal limb wounds. METHODS: In this experimental study of 10 Standardbred horses, five full‐thickness skin wounds (2 × 1.5 cm) were created on one metacarpus and six similar wounds were created on the contralateral metacarpus. Wounds were assigned to three groups: non‐contaminated control wounds; contaminated control wounds; contaminated wounds treated daily with 1 mL Manuka honey gel topically for 10 days. For the contaminated wounds, faeces were applied for 24 h after wound creation. In five horses wounds were bandaged and in the other five horses wounds were left without a bandage. Biopsies were taken on days 1, 2, 7 and 10 after wounding to evaluate the effects of Manuka honey gel, wound contamination and bandaging on TGF‐β1 and TGF‐β3 concentrations, aerobic and anaerobic bacterial counts, and histomorphology. RESULTS: Manuka honey gel had no significant effect on TGF‐β1 and TGF‐β3 concentrations or wound bacterial counts. Manuka honey gel decreased wound inflammation (days 7, 10), increased angiogenesis (days 2, 7, 10), increased fibrosis and collagen organisation (day 7) and increased epithelial hyperplasia (days 7, 10). CONCLUSIONS: Treatment with Manuka honey gel resulted in a more organised granulation tissue bed early in wound repair, which may contribute to enhanced healing of equine distal limb wounds.
doi_str_mv 10.1111/avj.12405
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1761078959</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1761078959</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3375-d6319f8a6f8be10ca1f2794ee2afa9db896e2b20fc4f97921181af2fed3be9ff3</originalsourceid><addsrcrecordid>eNp1kUtu1TAUhiMEopfCgA2Ah0Uire08PayqUuiLAbQMLcc5Ttyb2Le2w-XOWAJrYQdsgEV0JbhN2xmWrGPJ3_ksnz9JXhO8S-LaE9-vdgnNcfEkWZCqICmuSfY0WWCMixTnNNtKXnh_hXFWFbR4nmzRsiY5Kdgi-XOoFMiArEJnwkxLgXprYIM6GJA1KPSAghPGK-tGbTrUObsOPVJCBuvQ398ECdPGmiFpjQQT4aCt8e9RExFwWgzxZjLB34G99sGO1q16O9huc_ts7AsiukWAFqlpGG5-_gq9lksD3iO_1Aato6D1KJ7getIGUBs1UTzosfEvk2dKDB5e3dft5OLD4deDj-np56NPB_unqczit9O2zAhTtShV3QDBUhBFK5YDUKEEa5ualUAbipXMFasYJaQmQlEFbdYAUyrbTnZm78rZ6wl84KP2EoZBGLCT56QqCa5qVrCIvptR6az3DhRfOT0Kt-EE89vEeEyM3yUW2Tf32qkZoX0kHyKKwN4MrPUAm_-b-P7l8YMynTvilODHY4dwS15WcRb82_kRPzu_rPHxCeF55N_OvBKWi85pzy--UExKHHeJ8yr7BzLWv_w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1761078959</pqid></control><display><type>article</type><title>Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Bischofberger, AS ; Dart, CM ; Horadagoda, N ; Perkins, NR ; Jeffcott, LB ; Little, CB ; Dart, AJ</creator><creatorcontrib>Bischofberger, AS ; Dart, CM ; Horadagoda, N ; Perkins, NR ; Jeffcott, LB ; Little, CB ; Dart, AJ</creatorcontrib><description>OBJECTIVE: To investigate the effect of 66% Manuka honey gel on the concentrations of transforming growth factor (TGF)‐β1 and TGF‐β3, bacterial counts and histomorphology during healing of contaminated equine distal limb wounds. METHODS: In this experimental study of 10 Standardbred horses, five full‐thickness skin wounds (2 × 1.5 cm) were created on one metacarpus and six similar wounds were created on the contralateral metacarpus. Wounds were assigned to three groups: non‐contaminated control wounds; contaminated control wounds; contaminated wounds treated daily with 1 mL Manuka honey gel topically for 10 days. For the contaminated wounds, faeces were applied for 24 h after wound creation. In five horses wounds were bandaged and in the other five horses wounds were left without a bandage. Biopsies were taken on days 1, 2, 7 and 10 after wounding to evaluate the effects of Manuka honey gel, wound contamination and bandaging on TGF‐β1 and TGF‐β3 concentrations, aerobic and anaerobic bacterial counts, and histomorphology. RESULTS: Manuka honey gel had no significant effect on TGF‐β1 and TGF‐β3 concentrations or wound bacterial counts. Manuka honey gel decreased wound inflammation (days 7, 10), increased angiogenesis (days 2, 7, 10), increased fibrosis and collagen organisation (day 7) and increased epithelial hyperplasia (days 7, 10). CONCLUSIONS: Treatment with Manuka honey gel resulted in a more organised granulation tissue bed early in wound repair, which may contribute to enhanced healing of equine distal limb wounds.</description><identifier>ISSN: 0005-0423</identifier><identifier>EISSN: 1751-0813</identifier><identifier>DOI: 10.1111/avj.12405</identifier><identifier>PMID: 26814159</identifier><language>eng</language><publisher>Melbourne: Wiley Publishing Asia Pty Ltd</publisher><subject>Administration, Topical ; Animals ; Bacterial Load ; Extremities ; Feces - microbiology ; Honey ; Horse Diseases - metabolism ; Horse Diseases - microbiology ; Horse Diseases - therapy ; Horses ; Manuka honey gel ; Skin - injuries ; Skin - microbiology ; skin wounds ; transforming growth factor beta ; Transforming Growth Factor beta1 - metabolism ; Transforming Growth Factor beta3 - metabolism ; Wound Healing</subject><ispartof>Australian veterinary journal, 2016, Vol.94 (1-2), p.27-34</ispartof><rights>2016 Australian Veterinary Association</rights><rights>2016 Australian Veterinary Association.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3375-d6319f8a6f8be10ca1f2794ee2afa9db896e2b20fc4f97921181af2fed3be9ff3</citedby><cites>FETCH-LOGICAL-c3375-d6319f8a6f8be10ca1f2794ee2afa9db896e2b20fc4f97921181af2fed3be9ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Favj.12405$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Favj.12405$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26814159$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bischofberger, AS</creatorcontrib><creatorcontrib>Dart, CM</creatorcontrib><creatorcontrib>Horadagoda, N</creatorcontrib><creatorcontrib>Perkins, NR</creatorcontrib><creatorcontrib>Jeffcott, LB</creatorcontrib><creatorcontrib>Little, CB</creatorcontrib><creatorcontrib>Dart, AJ</creatorcontrib><title>Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs</title><title>Australian veterinary journal</title><addtitle>Aust Vet J</addtitle><description>OBJECTIVE: To investigate the effect of 66% Manuka honey gel on the concentrations of transforming growth factor (TGF)‐β1 and TGF‐β3, bacterial counts and histomorphology during healing of contaminated equine distal limb wounds. METHODS: In this experimental study of 10 Standardbred horses, five full‐thickness skin wounds (2 × 1.5 cm) were created on one metacarpus and six similar wounds were created on the contralateral metacarpus. Wounds were assigned to three groups: non‐contaminated control wounds; contaminated control wounds; contaminated wounds treated daily with 1 mL Manuka honey gel topically for 10 days. For the contaminated wounds, faeces were applied for 24 h after wound creation. In five horses wounds were bandaged and in the other five horses wounds were left without a bandage. Biopsies were taken on days 1, 2, 7 and 10 after wounding to evaluate the effects of Manuka honey gel, wound contamination and bandaging on TGF‐β1 and TGF‐β3 concentrations, aerobic and anaerobic bacterial counts, and histomorphology. RESULTS: Manuka honey gel had no significant effect on TGF‐β1 and TGF‐β3 concentrations or wound bacterial counts. Manuka honey gel decreased wound inflammation (days 7, 10), increased angiogenesis (days 2, 7, 10), increased fibrosis and collagen organisation (day 7) and increased epithelial hyperplasia (days 7, 10). CONCLUSIONS: Treatment with Manuka honey gel resulted in a more organised granulation tissue bed early in wound repair, which may contribute to enhanced healing of equine distal limb wounds.</description><subject>Administration, Topical</subject><subject>Animals</subject><subject>Bacterial Load</subject><subject>Extremities</subject><subject>Feces - microbiology</subject><subject>Honey</subject><subject>Horse Diseases - metabolism</subject><subject>Horse Diseases - microbiology</subject><subject>Horse Diseases - therapy</subject><subject>Horses</subject><subject>Manuka honey gel</subject><subject>Skin - injuries</subject><subject>Skin - microbiology</subject><subject>skin wounds</subject><subject>transforming growth factor beta</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Transforming Growth Factor beta3 - metabolism</subject><subject>Wound Healing</subject><issn>0005-0423</issn><issn>1751-0813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtu1TAUhiMEopfCgA2Ah0Uire08PayqUuiLAbQMLcc5Ttyb2Le2w-XOWAJrYQdsgEV0JbhN2xmWrGPJ3_ksnz9JXhO8S-LaE9-vdgnNcfEkWZCqICmuSfY0WWCMixTnNNtKXnh_hXFWFbR4nmzRsiY5Kdgi-XOoFMiArEJnwkxLgXprYIM6GJA1KPSAghPGK-tGbTrUObsOPVJCBuvQ398ECdPGmiFpjQQT4aCt8e9RExFwWgzxZjLB34G99sGO1q16O9huc_ts7AsiukWAFqlpGG5-_gq9lksD3iO_1Aato6D1KJ7getIGUBs1UTzosfEvk2dKDB5e3dft5OLD4deDj-np56NPB_unqczit9O2zAhTtShV3QDBUhBFK5YDUKEEa5ualUAbipXMFasYJaQmQlEFbdYAUyrbTnZm78rZ6wl84KP2EoZBGLCT56QqCa5qVrCIvptR6az3DhRfOT0Kt-EE89vEeEyM3yUW2Tf32qkZoX0kHyKKwN4MrPUAm_-b-P7l8YMynTvilODHY4dwS15WcRb82_kRPzu_rPHxCeF55N_OvBKWi85pzy--UExKHHeJ8yr7BzLWv_w</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Bischofberger, AS</creator><creator>Dart, CM</creator><creator>Horadagoda, N</creator><creator>Perkins, NR</creator><creator>Jeffcott, LB</creator><creator>Little, CB</creator><creator>Dart, AJ</creator><general>Wiley Publishing Asia Pty Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2016</creationdate><title>Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs</title><author>Bischofberger, AS ; Dart, CM ; Horadagoda, N ; Perkins, NR ; Jeffcott, LB ; Little, CB ; Dart, AJ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3375-d6319f8a6f8be10ca1f2794ee2afa9db896e2b20fc4f97921181af2fed3be9ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Administration, Topical</topic><topic>Animals</topic><topic>Bacterial Load</topic><topic>Extremities</topic><topic>Feces - microbiology</topic><topic>Honey</topic><topic>Horse Diseases - metabolism</topic><topic>Horse Diseases - microbiology</topic><topic>Horse Diseases - therapy</topic><topic>Horses</topic><topic>Manuka honey gel</topic><topic>Skin - injuries</topic><topic>Skin - microbiology</topic><topic>skin wounds</topic><topic>transforming growth factor beta</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>Transforming Growth Factor beta3 - metabolism</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bischofberger, AS</creatorcontrib><creatorcontrib>Dart, CM</creatorcontrib><creatorcontrib>Horadagoda, N</creatorcontrib><creatorcontrib>Perkins, NR</creatorcontrib><creatorcontrib>Jeffcott, LB</creatorcontrib><creatorcontrib>Little, CB</creatorcontrib><creatorcontrib>Dart, AJ</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Australian veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bischofberger, AS</au><au>Dart, CM</au><au>Horadagoda, N</au><au>Perkins, NR</au><au>Jeffcott, LB</au><au>Little, CB</au><au>Dart, AJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs</atitle><jtitle>Australian veterinary journal</jtitle><addtitle>Aust Vet J</addtitle><date>2016</date><risdate>2016</risdate><volume>94</volume><issue>1-2</issue><spage>27</spage><epage>34</epage><pages>27-34</pages><issn>0005-0423</issn><eissn>1751-0813</eissn><abstract>OBJECTIVE: To investigate the effect of 66% Manuka honey gel on the concentrations of transforming growth factor (TGF)‐β1 and TGF‐β3, bacterial counts and histomorphology during healing of contaminated equine distal limb wounds. METHODS: In this experimental study of 10 Standardbred horses, five full‐thickness skin wounds (2 × 1.5 cm) were created on one metacarpus and six similar wounds were created on the contralateral metacarpus. Wounds were assigned to three groups: non‐contaminated control wounds; contaminated control wounds; contaminated wounds treated daily with 1 mL Manuka honey gel topically for 10 days. For the contaminated wounds, faeces were applied for 24 h after wound creation. In five horses wounds were bandaged and in the other five horses wounds were left without a bandage. Biopsies were taken on days 1, 2, 7 and 10 after wounding to evaluate the effects of Manuka honey gel, wound contamination and bandaging on TGF‐β1 and TGF‐β3 concentrations, aerobic and anaerobic bacterial counts, and histomorphology. RESULTS: Manuka honey gel had no significant effect on TGF‐β1 and TGF‐β3 concentrations or wound bacterial counts. Manuka honey gel decreased wound inflammation (days 7, 10), increased angiogenesis (days 2, 7, 10), increased fibrosis and collagen organisation (day 7) and increased epithelial hyperplasia (days 7, 10). CONCLUSIONS: Treatment with Manuka honey gel resulted in a more organised granulation tissue bed early in wound repair, which may contribute to enhanced healing of equine distal limb wounds.</abstract><cop>Melbourne</cop><pub>Wiley Publishing Asia Pty Ltd</pub><pmid>26814159</pmid><doi>10.1111/avj.12405</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0005-0423
ispartof Australian veterinary journal, 2016, Vol.94 (1-2), p.27-34
issn 0005-0423
1751-0813
language eng
recordid cdi_proquest_miscellaneous_1761078959
source MEDLINE; Access via Wiley Online Library
subjects Administration, Topical
Animals
Bacterial Load
Extremities
Feces - microbiology
Honey
Horse Diseases - metabolism
Horse Diseases - microbiology
Horse Diseases - therapy
Horses
Manuka honey gel
Skin - injuries
Skin - microbiology
skin wounds
transforming growth factor beta
Transforming Growth Factor beta1 - metabolism
Transforming Growth Factor beta3 - metabolism
Wound Healing
title Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T18%3A00%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Manuka%20honey%20gel%20on%20the%20transforming%20growth%20factor%20%CE%B21%20and%20%CE%B23%20concentrations,%20bacterial%20counts%20and%20histomorphology%20of%20contaminated%20full%E2%80%90thickness%20skin%20wounds%20in%20equine%20distal%20limbs&rft.jtitle=Australian%20veterinary%20journal&rft.au=Bischofberger,%20AS&rft.date=2016&rft.volume=94&rft.issue=1-2&rft.spage=27&rft.epage=34&rft.pages=27-34&rft.issn=0005-0423&rft.eissn=1751-0813&rft_id=info:doi/10.1111/avj.12405&rft_dat=%3Cproquest_cross%3E1761078959%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1761078959&rft_id=info:pmid/26814159&rfr_iscdi=true