Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma
Glycosaminoglycans (GAGs) are heterogeneous, linear, highly charged, anionic polysaccharides consisting of repeating disaccharides units. GAGs have some biological significance in cancer progression (invasion and metastasis) and cell signaling. In different cancer types, GAGs undergo specific struct...
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| Veröffentlicht in: | Glycoconjugate journal 2016-02, Vol.33 (1), p.103-112 |
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| creator | Ucakturk, Ebru Akman, Orkun Sun, Xiaojun Baydar, Dilek Ertoy Dolgun, Anil Zhang, Fuming Linhardt, Robert J. |
| description | Glycosaminoglycans (GAGs) are heterogeneous, linear, highly charged, anionic polysaccharides consisting of repeating disaccharides units. GAGs have some biological significance in cancer progression (invasion and metastasis) and cell signaling. In different cancer types, GAGs undergo specific structural changes. In the present study, in depth investigation of changes in sulfation pattern and composition of GAGs, heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate and hyaluronan (HA) in normal renal tissue (NRT) and renal cell carcinoma tissue (RCCT) were evaluated. The statistical evaluation showed that alteration of the HS (HS
NRT
= 415.1 ± 115.3; HS
RCCT
= 277.5 ± 134.3), and CS (CS
NRT
= 35.3 ± 12.3; CS
RCCT
= 166.7 ± 108.8) amounts (in ng/mg dry tissue
)
were statistically significant (
p
|
| doi_str_mv | 10.1007/s10719-015-9643-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1760907027</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1760907027</sourcerecordid><originalsourceid>FETCH-LOGICAL-c508t-2292ba1d06f9588ad3b314d37bc8e933bb9ead4d9f9404480d159e95ba420f203</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMo7rr6A7xIwYuX6iRN0uQoi1-w4EUvXkLapmuXNlmT9uC_N92uIoKXfDDPOzM8CJ1juMYA-U3AkGOZAmap5DRL8QGaY5ZnKZWCH6I5EEFSAAEzdBLCBmKGEnGMZoRzTijnc_S2fNd2bULS2KR03daFpm-cTbStkjC0td79trrvjbchcXWybj9Lp7vGuvGl7S7qjdVtUpo2HtqXsdjpU3RU6zaYs_29QK_3dy_Lx3T1_PC0vF2lJQPRp4RIUmhcAa8lE0JXWZFhWmV5UQojs6wopNEVrWQtKVAqoMJMGskKTQnUBLIFupr6br37GEzoVdeEcRVtjRuCwjkHCTmQPKKXf9CNG3xcfaSYkBwzKiOFJ6r0LgRvarX1Taf9p8KgRvFqEq-ieDWKVzhmLvadh6Iz1U_i23QEyASEWIrG_a_R_3b9Aucjjds</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1758961549</pqid></control><display><type>article</type><title>Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Ucakturk, Ebru ; Akman, Orkun ; Sun, Xiaojun ; Baydar, Dilek Ertoy ; Dolgun, Anil ; Zhang, Fuming ; Linhardt, Robert J.</creator><creatorcontrib>Ucakturk, Ebru ; Akman, Orkun ; Sun, Xiaojun ; Baydar, Dilek Ertoy ; Dolgun, Anil ; Zhang, Fuming ; Linhardt, Robert J.</creatorcontrib><description>Glycosaminoglycans (GAGs) are heterogeneous, linear, highly charged, anionic polysaccharides consisting of repeating disaccharides units. GAGs have some biological significance in cancer progression (invasion and metastasis) and cell signaling. In different cancer types, GAGs undergo specific structural changes. In the present study, in depth investigation of changes in sulfation pattern and composition of GAGs, heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate and hyaluronan (HA) in normal renal tissue (NRT) and renal cell carcinoma tissue (RCCT) were evaluated. The statistical evaluation showed that alteration of the HS (HS
NRT
= 415.1 ± 115.3; HS
RCCT
= 277.5 ± 134.3), and CS (CS
NRT
= 35.3 ± 12.3; CS
RCCT
= 166.7 ± 108.8) amounts (in ng/mg dry tissue
)
were statistically significant (
p
< 0.05). Sulfation pattern in NRT and RCCT was evaluated to reveal disaccharide profiles. Statistical analyses showed that RCCT samples contain significantly increased amounts (in units of ng/mg dry tissue
)
of 4S
CS
(NRT = 25.7 ± 9.4; RCCT = 117.1 ± 73.9), SE
CS
(NRT = 0.7 ± 0.3; RCCT = 4.7 ± 4.5), 6S
CS
(NRT = 6.1 ± 2.7; RCCT = 39.4 ± 34.7) and significantly decreased amounts (in units of ng/mg dry tissue
)
of NS6S
HS
(RCCT = 28.6 ± 6.5, RCCT = 10.2 ± 8.0), NS2S
HS
(RCCT = 44.2 ± 13.8; RCCT = 27.2 ± 15.0), NS
HS
(NRT = 68.4 ± 15.8; RCCT = 50.4 ± 21.2), 2S6S
HS
(NRT = 1.0 ± 0.4; RCCT = 0.4 ± 0.3), and 6S
HS
(NRT = 60.6 ± 17.5; RCCT = 24.9 ± 12.3). If these changes in GAGs are proven to be specific and sensitive, they may serve as potential biomarkers in RCC. Our findings are likely to help us to show the direction for further investigations to be able to bring different diagnostic and prognostic approaches in renal tumors.</description><identifier>ISSN: 0282-0080</identifier><identifier>EISSN: 1573-4986</identifier><identifier>DOI: 10.1007/s10719-015-9643-1</identifier><identifier>PMID: 26662466</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biomarkers ; Biomarkers, Tumor - metabolism ; Biomedical and Life Sciences ; Carbohydrates ; Carcinoma, Renal Cell - metabolism ; Carcinoma, Renal Cell - pathology ; Chondroitin Sulfates - metabolism ; Dermatan Sulfate - metabolism ; Heparitin Sulfate - metabolism ; Humans ; Kidney cancer ; Kidney Neoplasms - metabolism ; Kidney Neoplasms - pathology ; Life Sciences ; Original Article ; Pathology</subject><ispartof>Glycoconjugate journal, 2016-02, Vol.33 (1), p.103-112</ispartof><rights>Springer Science+Business Media New York 2015</rights><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-2292ba1d06f9588ad3b314d37bc8e933bb9ead4d9f9404480d159e95ba420f203</citedby><cites>FETCH-LOGICAL-c508t-2292ba1d06f9588ad3b314d37bc8e933bb9ead4d9f9404480d159e95ba420f203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10719-015-9643-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10719-015-9643-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26662466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ucakturk, Ebru</creatorcontrib><creatorcontrib>Akman, Orkun</creatorcontrib><creatorcontrib>Sun, Xiaojun</creatorcontrib><creatorcontrib>Baydar, Dilek Ertoy</creatorcontrib><creatorcontrib>Dolgun, Anil</creatorcontrib><creatorcontrib>Zhang, Fuming</creatorcontrib><creatorcontrib>Linhardt, Robert J.</creatorcontrib><title>Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma</title><title>Glycoconjugate journal</title><addtitle>Glycoconj J</addtitle><addtitle>Glycoconj J</addtitle><description>Glycosaminoglycans (GAGs) are heterogeneous, linear, highly charged, anionic polysaccharides consisting of repeating disaccharides units. GAGs have some biological significance in cancer progression (invasion and metastasis) and cell signaling. In different cancer types, GAGs undergo specific structural changes. In the present study, in depth investigation of changes in sulfation pattern and composition of GAGs, heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate and hyaluronan (HA) in normal renal tissue (NRT) and renal cell carcinoma tissue (RCCT) were evaluated. The statistical evaluation showed that alteration of the HS (HS
NRT
= 415.1 ± 115.3; HS
RCCT
= 277.5 ± 134.3), and CS (CS
NRT
= 35.3 ± 12.3; CS
RCCT
= 166.7 ± 108.8) amounts (in ng/mg dry tissue
)
were statistically significant (
p
< 0.05). Sulfation pattern in NRT and RCCT was evaluated to reveal disaccharide profiles. Statistical analyses showed that RCCT samples contain significantly increased amounts (in units of ng/mg dry tissue
)
of 4S
CS
(NRT = 25.7 ± 9.4; RCCT = 117.1 ± 73.9), SE
CS
(NRT = 0.7 ± 0.3; RCCT = 4.7 ± 4.5), 6S
CS
(NRT = 6.1 ± 2.7; RCCT = 39.4 ± 34.7) and significantly decreased amounts (in units of ng/mg dry tissue
)
of NS6S
HS
(RCCT = 28.6 ± 6.5, RCCT = 10.2 ± 8.0), NS2S
HS
(RCCT = 44.2 ± 13.8; RCCT = 27.2 ± 15.0), NS
HS
(NRT = 68.4 ± 15.8; RCCT = 50.4 ± 21.2), 2S6S
HS
(NRT = 1.0 ± 0.4; RCCT = 0.4 ± 0.3), and 6S
HS
(NRT = 60.6 ± 17.5; RCCT = 24.9 ± 12.3). If these changes in GAGs are proven to be specific and sensitive, they may serve as potential biomarkers in RCC. Our findings are likely to help us to show the direction for further investigations to be able to bring different diagnostic and prognostic approaches in renal tumors.</description><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Carbohydrates</subject><subject>Carcinoma, Renal Cell - metabolism</subject><subject>Carcinoma, Renal Cell - pathology</subject><subject>Chondroitin Sulfates - metabolism</subject><subject>Dermatan Sulfate - metabolism</subject><subject>Heparitin Sulfate - metabolism</subject><subject>Humans</subject><subject>Kidney cancer</subject><subject>Kidney Neoplasms - metabolism</subject><subject>Kidney Neoplasms - pathology</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Pathology</subject><issn>0282-0080</issn><issn>1573-4986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1LxDAQhoMo7rr6A7xIwYuX6iRN0uQoi1-w4EUvXkLapmuXNlmT9uC_N92uIoKXfDDPOzM8CJ1juMYA-U3AkGOZAmap5DRL8QGaY5ZnKZWCH6I5EEFSAAEzdBLCBmKGEnGMZoRzTijnc_S2fNd2bULS2KR03daFpm-cTbStkjC0td79trrvjbchcXWybj9Lp7vGuvGl7S7qjdVtUpo2HtqXsdjpU3RU6zaYs_29QK_3dy_Lx3T1_PC0vF2lJQPRp4RIUmhcAa8lE0JXWZFhWmV5UQojs6wopNEVrWQtKVAqoMJMGskKTQnUBLIFupr6br37GEzoVdeEcRVtjRuCwjkHCTmQPKKXf9CNG3xcfaSYkBwzKiOFJ6r0LgRvarX1Taf9p8KgRvFqEq-ieDWKVzhmLvadh6Iz1U_i23QEyASEWIrG_a_R_3b9Aucjjds</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Ucakturk, Ebru</creator><creator>Akman, Orkun</creator><creator>Sun, Xiaojun</creator><creator>Baydar, Dilek Ertoy</creator><creator>Dolgun, Anil</creator><creator>Zhang, Fuming</creator><creator>Linhardt, Robert J.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20160201</creationdate><title>Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma</title><author>Ucakturk, Ebru ; Akman, Orkun ; Sun, Xiaojun ; Baydar, Dilek Ertoy ; Dolgun, Anil ; Zhang, Fuming ; Linhardt, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-2292ba1d06f9588ad3b314d37bc8e933bb9ead4d9f9404480d159e95ba420f203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Carbohydrates</topic><topic>Carcinoma, Renal Cell - metabolism</topic><topic>Carcinoma, Renal Cell - pathology</topic><topic>Chondroitin Sulfates - metabolism</topic><topic>Dermatan Sulfate - metabolism</topic><topic>Heparitin Sulfate - metabolism</topic><topic>Humans</topic><topic>Kidney cancer</topic><topic>Kidney Neoplasms - metabolism</topic><topic>Kidney Neoplasms - pathology</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ucakturk, Ebru</creatorcontrib><creatorcontrib>Akman, Orkun</creatorcontrib><creatorcontrib>Sun, Xiaojun</creatorcontrib><creatorcontrib>Baydar, Dilek Ertoy</creatorcontrib><creatorcontrib>Dolgun, Anil</creatorcontrib><creatorcontrib>Zhang, Fuming</creatorcontrib><creatorcontrib>Linhardt, Robert J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Glycoconjugate journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ucakturk, Ebru</au><au>Akman, Orkun</au><au>Sun, Xiaojun</au><au>Baydar, Dilek Ertoy</au><au>Dolgun, Anil</au><au>Zhang, Fuming</au><au>Linhardt, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma</atitle><jtitle>Glycoconjugate journal</jtitle><stitle>Glycoconj J</stitle><addtitle>Glycoconj J</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>33</volume><issue>1</issue><spage>103</spage><epage>112</epage><pages>103-112</pages><issn>0282-0080</issn><eissn>1573-4986</eissn><abstract>Glycosaminoglycans (GAGs) are heterogeneous, linear, highly charged, anionic polysaccharides consisting of repeating disaccharides units. GAGs have some biological significance in cancer progression (invasion and metastasis) and cell signaling. In different cancer types, GAGs undergo specific structural changes. In the present study, in depth investigation of changes in sulfation pattern and composition of GAGs, heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate and hyaluronan (HA) in normal renal tissue (NRT) and renal cell carcinoma tissue (RCCT) were evaluated. The statistical evaluation showed that alteration of the HS (HS
NRT
= 415.1 ± 115.3; HS
RCCT
= 277.5 ± 134.3), and CS (CS
NRT
= 35.3 ± 12.3; CS
RCCT
= 166.7 ± 108.8) amounts (in ng/mg dry tissue
)
were statistically significant (
p
< 0.05). Sulfation pattern in NRT and RCCT was evaluated to reveal disaccharide profiles. Statistical analyses showed that RCCT samples contain significantly increased amounts (in units of ng/mg dry tissue
)
of 4S
CS
(NRT = 25.7 ± 9.4; RCCT = 117.1 ± 73.9), SE
CS
(NRT = 0.7 ± 0.3; RCCT = 4.7 ± 4.5), 6S
CS
(NRT = 6.1 ± 2.7; RCCT = 39.4 ± 34.7) and significantly decreased amounts (in units of ng/mg dry tissue
)
of NS6S
HS
(RCCT = 28.6 ± 6.5, RCCT = 10.2 ± 8.0), NS2S
HS
(RCCT = 44.2 ± 13.8; RCCT = 27.2 ± 15.0), NS
HS
(NRT = 68.4 ± 15.8; RCCT = 50.4 ± 21.2), 2S6S
HS
(NRT = 1.0 ± 0.4; RCCT = 0.4 ± 0.3), and 6S
HS
(NRT = 60.6 ± 17.5; RCCT = 24.9 ± 12.3). If these changes in GAGs are proven to be specific and sensitive, they may serve as potential biomarkers in RCC. Our findings are likely to help us to show the direction for further investigations to be able to bring different diagnostic and prognostic approaches in renal tumors.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26662466</pmid><doi>10.1007/s10719-015-9643-1</doi><tpages>10</tpages></addata></record> |
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| subjects | Biochemistry Biomarkers Biomarkers, Tumor - metabolism Biomedical and Life Sciences Carbohydrates Carcinoma, Renal Cell - metabolism Carcinoma, Renal Cell - pathology Chondroitin Sulfates - metabolism Dermatan Sulfate - metabolism Heparitin Sulfate - metabolism Humans Kidney cancer Kidney Neoplasms - metabolism Kidney Neoplasms - pathology Life Sciences Original Article Pathology |
| title | Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma |
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