PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin, and agr genes in Staphylococcus aureus strains isolated from psoriasis patients
Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S...
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| Veröffentlicht in: | Turkish journal of medical sciences 2015, Vol.45 (6), p.1345-1352 |
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| container_title | Turkish journal of medical sciences |
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| creator | Göçmen Jülide Sedef Sahiner, Neriman Koçak, Mukadder Karahan, Zeynep Ceren |
| description | Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nares of psoriasis patients and healthy controls.
The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR.
Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci.
Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis. |
| doi_str_mv | 10.3906/sag-1408-54 |
| format | Article |
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The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR.
Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci.
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The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR.
Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci.
Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Toxins - genetics</subject><subject>Case-Control Studies</subject><subject>Enterotoxins - genetics</subject><subject>Exfoliatins - genetics</subject><subject>Exotoxins - genetics</subject><subject>Humans</subject><subject>Leukocidins - genetics</subject><subject>Nose - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Psoriasis - microbiology</subject><subject>Skin - microbiology</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Trans-Activators - genetics</subject><issn>1300-0144</issn><issn>1303-6165</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU1PFTEUhhuiAURX7kmXJjrSTr9mluZG0IREAuh2cm6nvRbmtkNPh8DP8Z9a4OrqfOQ570nel5D3nH0WPdMnCJuGS9Y1Su6RQy6YaDTX6tVzzxrGpTwgbxBvGGuFVP0-OWi1MUr04pD8uVhd0hDvHZawgRJSpMnTC4glxeYXTC6WEB2d3HKbbBhD_ETryuVU0sPz8ODTFOrhvaO7FcSRwibTjYsOqza9KjD_fpySTdYuSGHJrhYsGUKsAKYJihupz2lLZ0w5AAakcxWtr_Atee1hQvduV4_Iz9Ov16tvzfmPs--rL-eNFa0ujesMl6CkA68Mh7Fr16I3bcu91cx2a81G3vXKV6M6I0bZGuOrBSC04V5pIY7IhxfdOae7pfoxbANaN00QXVpw4Eazrm-r5RX9-ILanBCz88Ocwxby48DZ8JTJUDMZnjIZlKz08U54WW_d-J_9F4L4C0tviso</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Göçmen Jülide Sedef</creator><creator>Sahiner, Neriman</creator><creator>Koçak, Mukadder</creator><creator>Karahan, Zeynep Ceren</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2015</creationdate><title>PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin, and agr genes in Staphylococcus aureus strains isolated from psoriasis patients</title><author>Göçmen Jülide Sedef ; Sahiner, Neriman ; Koçak, Mukadder ; Karahan, Zeynep Ceren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-e8714a54eaf571ad82b397221fc60c8b60d1895f408873d4277f539a3671f5633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Toxins - genetics</topic><topic>Case-Control Studies</topic><topic>Enterotoxins - genetics</topic><topic>Exfoliatins - genetics</topic><topic>Exotoxins - genetics</topic><topic>Humans</topic><topic>Leukocidins - genetics</topic><topic>Nose - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Psoriasis - microbiology</topic><topic>Skin - microbiology</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Trans-Activators - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Göçmen Jülide Sedef</creatorcontrib><creatorcontrib>Sahiner, Neriman</creatorcontrib><creatorcontrib>Koçak, Mukadder</creatorcontrib><creatorcontrib>Karahan, Zeynep Ceren</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Turkish journal of medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Göçmen Jülide Sedef</au><au>Sahiner, Neriman</au><au>Koçak, Mukadder</au><au>Karahan, Zeynep Ceren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin, and agr genes in Staphylococcus aureus strains isolated from psoriasis patients</atitle><jtitle>Turkish journal of medical sciences</jtitle><addtitle>Turk J Med Sci</addtitle><date>2015</date><risdate>2015</risdate><volume>45</volume><issue>6</issue><spage>1345</spage><epage>1352</epage><pages>1345-1352</pages><issn>1300-0144</issn><eissn>1303-6165</eissn><abstract>Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nares of psoriasis patients and healthy controls.
The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR.
Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci.
Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.</abstract><cop>Turkey</cop><pmid>26775393</pmid><doi>10.3906/sag-1408-54</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Turkish journal of medical sciences, 2015, Vol.45 (6), p.1345-1352 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; TÜBİTAK Scientific Journals |
| subjects | Bacterial Proteins - genetics Bacterial Toxins - genetics Case-Control Studies Enterotoxins - genetics Exfoliatins - genetics Exotoxins - genetics Humans Leukocidins - genetics Nose - microbiology Polymerase Chain Reaction Psoriasis - microbiology Skin - microbiology Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Trans-Activators - genetics |
| title | PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin, and agr genes in Staphylococcus aureus strains isolated from psoriasis patients |
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