Carbon Nanodot-Decorated Ag@SiO2 Nanoparticles for Fluorescence and Surface-Enhanced Raman Scattering Immunoassays
A novel immunoassay protocol was demonstrated by the combination of fluorescent carbon nanodots (CNDs) and Ag@SiO2 surface-enhanced Raman scattering (SERS) tag nanoparticles into ensembles for a bifunctional nanoplatform. The CND-decorated Ag@SiO2 nanoparticles were constructed for sensitive fluores...
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| Veröffentlicht in: | ACS applied materials & interfaces 2016-01, Vol.8 (1), p.1033-1040 |
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| container_title | ACS applied materials & interfaces |
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| creator | Zhang, Xianfeng Du, Xuezhong |
| description | A novel immunoassay protocol was demonstrated by the combination of fluorescent carbon nanodots (CNDs) and Ag@SiO2 surface-enhanced Raman scattering (SERS) tag nanoparticles into ensembles for a bifunctional nanoplatform. The CND-decorated Ag@SiO2 nanoparticles were constructed for sensitive fluorescence and SERS immunoassays. The silica shell thickness and amount of Ag@SiO2 nanoparticles were optimized for availability of strong fluorescence emission. The considerably large Raman scattering cross section of in situ-generated actual Raman reporter, 4,4′-dimercaptoazobenzene, from the apparent reporter p-aminothiophenol modified on the surfaces of Ag nanoparticles upon illumination of laser compensated for the reduction of SERS signals resulting from silica coating to a great degree. The antibody-modified bifunctional nanoparticles were captured by antibody-modified quartz slides in the presence of antigens in the sandwich structures for fluorescence and SERS immunoassays. The bifunctional nanoparticles could be used not only as bimodal probes for biodetection but also as bimodal tracers for bioimaging. |
| doi_str_mv | 10.1021/acsami.5b11446 |
| format | Article |
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The CND-decorated Ag@SiO2 nanoparticles were constructed for sensitive fluorescence and SERS immunoassays. The silica shell thickness and amount of Ag@SiO2 nanoparticles were optimized for availability of strong fluorescence emission. The considerably large Raman scattering cross section of in situ-generated actual Raman reporter, 4,4′-dimercaptoazobenzene, from the apparent reporter p-aminothiophenol modified on the surfaces of Ag nanoparticles upon illumination of laser compensated for the reduction of SERS signals resulting from silica coating to a great degree. The antibody-modified bifunctional nanoparticles were captured by antibody-modified quartz slides in the presence of antigens in the sandwich structures for fluorescence and SERS immunoassays. The bifunctional nanoparticles could be used not only as bimodal probes for biodetection but also as bimodal tracers for bioimaging.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.5b11446</identifier><identifier>PMID: 26692186</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Carbon - chemistry ; Immunoassay - methods ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - ultrastructure ; Silicon Dioxide - chemistry ; Silver - chemistry ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet ; Spectrum Analysis, Raman - methods ; Static Electricity</subject><ispartof>ACS applied materials & interfaces, 2016-01, Vol.8 (1), p.1033-1040</ispartof><rights>Copyright © 2015 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.5b11446$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.5b11446$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26692186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xianfeng</creatorcontrib><creatorcontrib>Du, Xuezhong</creatorcontrib><title>Carbon Nanodot-Decorated Ag@SiO2 Nanoparticles for Fluorescence and Surface-Enhanced Raman Scattering Immunoassays</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>A novel immunoassay protocol was demonstrated by the combination of fluorescent carbon nanodots (CNDs) and Ag@SiO2 surface-enhanced Raman scattering (SERS) tag nanoparticles into ensembles for a bifunctional nanoplatform. The CND-decorated Ag@SiO2 nanoparticles were constructed for sensitive fluorescence and SERS immunoassays. The silica shell thickness and amount of Ag@SiO2 nanoparticles were optimized for availability of strong fluorescence emission. The considerably large Raman scattering cross section of in situ-generated actual Raman reporter, 4,4′-dimercaptoazobenzene, from the apparent reporter p-aminothiophenol modified on the surfaces of Ag nanoparticles upon illumination of laser compensated for the reduction of SERS signals resulting from silica coating to a great degree. The antibody-modified bifunctional nanoparticles were captured by antibody-modified quartz slides in the presence of antigens in the sandwich structures for fluorescence and SERS immunoassays. The bifunctional nanoparticles could be used not only as bimodal probes for biodetection but also as bimodal tracers for bioimaging.</description><subject>Carbon - chemistry</subject><subject>Immunoassay - methods</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Silicon Dioxide - chemistry</subject><subject>Silver - chemistry</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Static Electricity</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kcFLwzAUxoMobk6vHiVHEapJmqbZzTE3HQwHTs_hNUlnR5vMpD3sv7e66ek9vvfj8fF9CF1Tck8Jow-gIzTVfVZQyrk4QUM65jyRLGOn_zvnA3QR45YQkTKSnaMBE2LMqBRDFKYQCu_wKzhvfJs8We0DtNbgyeZxXa3Y72UHoa10bSMufcDzuvPBRm2dthicwesulKBtMnOf0GsGv0EDDq81tK0NldvgRdN0zkOMsI-X6KyEOtqr4xyhj_nsffqSLFfPi-lkmUBKWZukltO80BwYEMtkbkzKOBCZUprnFrQgvZBDxktjoOBjwyTIIs9FaUESSdIRuj383QX_1dnYqqbqTdc1OOu7qGguiJQyG2c9enNEu6KxRu1C1UDYq7-ceuDuAPR5q63vguudK0rUTwnqUII6lpB-A1ZFeZY</recordid><startdate>20160113</startdate><enddate>20160113</enddate><creator>Zhang, Xianfeng</creator><creator>Du, Xuezhong</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20160113</creationdate><title>Carbon Nanodot-Decorated Ag@SiO2 Nanoparticles for Fluorescence and Surface-Enhanced Raman Scattering Immunoassays</title><author>Zhang, Xianfeng ; Du, Xuezhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a312t-3e417bc4a2a0e287dd324a0831177eac60d327a54fddab49d28a8b776fea80803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Carbon - chemistry</topic><topic>Immunoassay - methods</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - ultrastructure</topic><topic>Silicon Dioxide - chemistry</topic><topic>Silver - chemistry</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Static Electricity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xianfeng</creatorcontrib><creatorcontrib>Du, Xuezhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xianfeng</au><au>Du, Xuezhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbon Nanodot-Decorated Ag@SiO2 Nanoparticles for Fluorescence and Surface-Enhanced Raman Scattering Immunoassays</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2016-01-13</date><risdate>2016</risdate><volume>8</volume><issue>1</issue><spage>1033</spage><epage>1040</epage><pages>1033-1040</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>A novel immunoassay protocol was demonstrated by the combination of fluorescent carbon nanodots (CNDs) and Ag@SiO2 surface-enhanced Raman scattering (SERS) tag nanoparticles into ensembles for a bifunctional nanoplatform. The CND-decorated Ag@SiO2 nanoparticles were constructed for sensitive fluorescence and SERS immunoassays. The silica shell thickness and amount of Ag@SiO2 nanoparticles were optimized for availability of strong fluorescence emission. The considerably large Raman scattering cross section of in situ-generated actual Raman reporter, 4,4′-dimercaptoazobenzene, from the apparent reporter p-aminothiophenol modified on the surfaces of Ag nanoparticles upon illumination of laser compensated for the reduction of SERS signals resulting from silica coating to a great degree. The antibody-modified bifunctional nanoparticles were captured by antibody-modified quartz slides in the presence of antigens in the sandwich structures for fluorescence and SERS immunoassays. The bifunctional nanoparticles could be used not only as bimodal probes for biodetection but also as bimodal tracers for bioimaging.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>26692186</pmid><doi>10.1021/acsami.5b11446</doi><tpages>8</tpages></addata></record> |
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| subjects | Carbon - chemistry Immunoassay - methods Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Silicon Dioxide - chemistry Silver - chemistry Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Spectrum Analysis, Raman - methods Static Electricity |
| title | Carbon Nanodot-Decorated Ag@SiO2 Nanoparticles for Fluorescence and Surface-Enhanced Raman Scattering Immunoassays |
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