The effect of high concentration isopropanol on the growth of a solvent-tolerant strain of Chlorella vulgaris
The discovery of a highly solvent-tolerant strain of microalga, identified as Chlorella vulgaris by 16S rRNA gene sequencing and termed SDC1 is described here for the first time. Ability to grow in the presence of isopropanol (IPA) solvent was assessed through specific growth rate ( μ) determination...
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| Veröffentlicht in: | Enzyme and microbial technology 2004-08, Vol.35 (2), p.140-146 |
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| creator | McEvoy, Eileen Wright, Phillip C Bustard, Mark T |
| description | The discovery of a highly solvent-tolerant strain of microalga, identified as
Chlorella vulgaris by 16S rRNA gene sequencing and termed SDC1 is described here for the first time. Ability to grow in the presence of isopropanol (IPA) solvent was assessed through specific growth rate (
μ) determination at IPA feed concentrations of up to 16
g
l
−1 at 20
°C. Specific growth rates between 0.0017 and 0.0038
h
−1 were obtained in the presence of 2–16
g
l
−1 IPA, and a value of 0.0047
h
−1 evident under IPA-free conditions. Axenic cultures of
C. vulgaris SDC1 also demonstrated heterotrophic bioconversion of IPA at these elevated concentrations, where acetone, the suggested metabolite, was monitored as an indicator of aerobic degradation of IPA. Comparison of
C. vulgaris SDC1 growth characteristics was carried out against that of the type culture strain,
C. vulgaris Beijerinck (CCAP211/11B), where the solvent-tolerant strain SDC1 displayed lower sensitivity to high IPA concentrations. An LD
50 value was found to occur at initial IPA concentrations of 3.8
g
l
−1 for the type strain in comparison with 11.25
g
l
−1 for the solvent-tolerant SDC1 strain. Despite several studies discussing the existence of solvent-tolerant bacteria, this is the first time solvent-tolerance at such high IPA concentrations (to 16
g
l
−1) has been successfully demonstrated by an isolated
C. vulgaris strain in a mineral salts medium. |
| doi_str_mv | 10.1016/j.enzmictec.2004.01.012 |
| format | Article |
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Chlorella vulgaris by 16S rRNA gene sequencing and termed SDC1 is described here for the first time. Ability to grow in the presence of isopropanol (IPA) solvent was assessed through specific growth rate (
μ) determination at IPA feed concentrations of up to 16
g
l
−1 at 20
°C. Specific growth rates between 0.0017 and 0.0038
h
−1 were obtained in the presence of 2–16
g
l
−1 IPA, and a value of 0.0047
h
−1 evident under IPA-free conditions. Axenic cultures of
C. vulgaris SDC1 also demonstrated heterotrophic bioconversion of IPA at these elevated concentrations, where acetone, the suggested metabolite, was monitored as an indicator of aerobic degradation of IPA. Comparison of
C. vulgaris SDC1 growth characteristics was carried out against that of the type culture strain,
C. vulgaris Beijerinck (CCAP211/11B), where the solvent-tolerant strain SDC1 displayed lower sensitivity to high IPA concentrations. An LD
50 value was found to occur at initial IPA concentrations of 3.8
g
l
−1 for the type strain in comparison with 11.25
g
l
−1 for the solvent-tolerant SDC1 strain. Despite several studies discussing the existence of solvent-tolerant bacteria, this is the first time solvent-tolerance at such high IPA concentrations (to 16
g
l
−1) has been successfully demonstrated by an isolated
C. vulgaris strain in a mineral salts medium.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/j.enzmictec.2004.01.012</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Algal culture (microalgae) ; Bioconversion ; Biological and medical sciences ; Biotechnology ; Chlorella vulgaris ; Fundamental and applied biological sciences. Psychology ; Isopropanol (IPA) ; Methods. Procedures. Technologies ; Microalgae ; Solvent-tolerant</subject><ispartof>Enzyme and microbial technology, 2004-08, Vol.35 (2), p.140-146</ispartof><rights>2004 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-724075627675c11bbf4e807dbf6907878467ed6df6baddff8b13af48f4cc89e23</citedby><cites>FETCH-LOGICAL-c374t-724075627675c11bbf4e807dbf6907878467ed6df6baddff8b13af48f4cc89e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141022904001085$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15972636$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>McEvoy, Eileen</creatorcontrib><creatorcontrib>Wright, Phillip C</creatorcontrib><creatorcontrib>Bustard, Mark T</creatorcontrib><title>The effect of high concentration isopropanol on the growth of a solvent-tolerant strain of Chlorella vulgaris</title><title>Enzyme and microbial technology</title><description>The discovery of a highly solvent-tolerant strain of microalga, identified as
Chlorella vulgaris by 16S rRNA gene sequencing and termed SDC1 is described here for the first time. Ability to grow in the presence of isopropanol (IPA) solvent was assessed through specific growth rate (
μ) determination at IPA feed concentrations of up to 16
g
l
−1 at 20
°C. Specific growth rates between 0.0017 and 0.0038
h
−1 were obtained in the presence of 2–16
g
l
−1 IPA, and a value of 0.0047
h
−1 evident under IPA-free conditions. Axenic cultures of
C. vulgaris SDC1 also demonstrated heterotrophic bioconversion of IPA at these elevated concentrations, where acetone, the suggested metabolite, was monitored as an indicator of aerobic degradation of IPA. Comparison of
C. vulgaris SDC1 growth characteristics was carried out against that of the type culture strain,
C. vulgaris Beijerinck (CCAP211/11B), where the solvent-tolerant strain SDC1 displayed lower sensitivity to high IPA concentrations. An LD
50 value was found to occur at initial IPA concentrations of 3.8
g
l
−1 for the type strain in comparison with 11.25
g
l
−1 for the solvent-tolerant SDC1 strain. Despite several studies discussing the existence of solvent-tolerant bacteria, this is the first time solvent-tolerance at such high IPA concentrations (to 16
g
l
−1) has been successfully demonstrated by an isolated
C. vulgaris strain in a mineral salts medium.</description><subject>Algal culture (microalgae)</subject><subject>Bioconversion</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chlorella vulgaris</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isopropanol (IPA)</subject><subject>Methods. Procedures. Technologies</subject><subject>Microalgae</subject><subject>Solvent-tolerant</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkMFqGzEQhkVoIa6bZ4guyW3d0a4s7R6NadpAoJf0LLTakVdGXjmS7NI8fbQ4NMfCwDDSN_PP_ITcMlgxYOLbfoXT68GZjGZVA_AVsBL1FVmwVnYVdNB9IgtgnFVQ1901-ZLSHqA8cFiQw_OIFK1Fk2mwdHS7kZowGZxy1NmFiboUjjEc9RQ8LWUu_C6GP3mceU1T8OcCVzl4jHrKNJVGN82f29GHiN5rej75nY4ufSWfrfYJb97zkvx--P68_Vk9_frxuN08VaaRPFey5iDXopZCrg1jfW85tiCH3ooOZCtbLiQOYrCi18NgbduzRlveWm5M22HdLMn9ZW7Z_OWEKauDS2ZeZcJwSopJAS00soDyApoYUopo1TG6g45_FQM126v26p-9arZXASsxS9y9S-hktLflduPSR_u6k7VoROE2Fw7LvWeHUSXjsBg8uFhMV0Nw_9V6AxvUlyw</recordid><startdate>20040805</startdate><enddate>20040805</enddate><creator>McEvoy, Eileen</creator><creator>Wright, Phillip C</creator><creator>Bustard, Mark T</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20040805</creationdate><title>The effect of high concentration isopropanol on the growth of a solvent-tolerant strain of Chlorella vulgaris</title><author>McEvoy, Eileen ; Wright, Phillip C ; Bustard, Mark T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-724075627675c11bbf4e807dbf6907878467ed6df6baddff8b13af48f4cc89e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Algal culture (microalgae)</topic><topic>Bioconversion</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chlorella vulgaris</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isopropanol (IPA)</topic><topic>Methods. Procedures. Technologies</topic><topic>Microalgae</topic><topic>Solvent-tolerant</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McEvoy, Eileen</creatorcontrib><creatorcontrib>Wright, Phillip C</creatorcontrib><creatorcontrib>Bustard, Mark T</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McEvoy, Eileen</au><au>Wright, Phillip C</au><au>Bustard, Mark T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of high concentration isopropanol on the growth of a solvent-tolerant strain of Chlorella vulgaris</atitle><jtitle>Enzyme and microbial technology</jtitle><date>2004-08-05</date><risdate>2004</risdate><volume>35</volume><issue>2</issue><spage>140</spage><epage>146</epage><pages>140-146</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>The discovery of a highly solvent-tolerant strain of microalga, identified as
Chlorella vulgaris by 16S rRNA gene sequencing and termed SDC1 is described here for the first time. Ability to grow in the presence of isopropanol (IPA) solvent was assessed through specific growth rate (
μ) determination at IPA feed concentrations of up to 16
g
l
−1 at 20
°C. Specific growth rates between 0.0017 and 0.0038
h
−1 were obtained in the presence of 2–16
g
l
−1 IPA, and a value of 0.0047
h
−1 evident under IPA-free conditions. Axenic cultures of
C. vulgaris SDC1 also demonstrated heterotrophic bioconversion of IPA at these elevated concentrations, where acetone, the suggested metabolite, was monitored as an indicator of aerobic degradation of IPA. Comparison of
C. vulgaris SDC1 growth characteristics was carried out against that of the type culture strain,
C. vulgaris Beijerinck (CCAP211/11B), where the solvent-tolerant strain SDC1 displayed lower sensitivity to high IPA concentrations. An LD
50 value was found to occur at initial IPA concentrations of 3.8
g
l
−1 for the type strain in comparison with 11.25
g
l
−1 for the solvent-tolerant SDC1 strain. Despite several studies discussing the existence of solvent-tolerant bacteria, this is the first time solvent-tolerance at such high IPA concentrations (to 16
g
l
−1) has been successfully demonstrated by an isolated
C. vulgaris strain in a mineral salts medium.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/j.enzmictec.2004.01.012</doi><tpages>7</tpages></addata></record> |
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| ispartof | Enzyme and microbial technology, 2004-08, Vol.35 (2), p.140-146 |
| issn | 0141-0229 1879-0909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17608037 |
| source | Elsevier ScienceDirect Journals |
| subjects | Algal culture (microalgae) Bioconversion Biological and medical sciences Biotechnology Chlorella vulgaris Fundamental and applied biological sciences. Psychology Isopropanol (IPA) Methods. Procedures. Technologies Microalgae Solvent-tolerant |
| title | The effect of high concentration isopropanol on the growth of a solvent-tolerant strain of Chlorella vulgaris |
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