Enhanced Interleukin-1β, Interleukin-6 and Tumor Necrosis Factor-α Production by LPS Stimulated Human Monocytes Isolated from HIV + Patients
Abstract Periodontal disease and tooth loss is a common finding among advanced HIV+ patients. In addition to local oral lipopolysaccharide (LPS) stimulation, systemic up-regulation of monocyte pro-inflammatory cytokine secretion may also be involved in the pathogenesis of HIV disease. A study was un...
Gespeichert in:
| Veröffentlicht in: | Immunopharmacology and immunotoxicology 2000-01, Vol.22 (3), p.401-421 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 421 |
|---|---|
| container_issue | 3 |
| container_start_page | 401 |
| container_title | Immunopharmacology and immunotoxicology |
| container_volume | 22 |
| creator | Baqui, A. A. M. A. Jabra-Rizk, Mary Ann Kelley, Jacqueline I. Zhang, Ming Falkler, William A. Meiller, Timothy F. |
| description | Abstract
Periodontal disease and tooth loss is a common finding among advanced HIV+ patients. In addition to local oral lipopolysaccharide (LPS) stimulation, systemic up-regulation of monocyte pro-inflammatory cytokine secretion may also be involved in the pathogenesis of HIV disease. A study was undertaken to investigate IL-1β, IL-6 and TNF-α production by resting and LPS stimulated monocytes isolated from HIV + patients and also to investigate the relationship of the patient's HIV viral load status to the cytokine production. Whole blood samples in EDTA were collected from 39 HIV-1 infected patients and 20 age and sex matched uninfected controls. Plasma was separated by centrifugation. Viral load was determined using a quantitative RT-PCR. Monocytes were isolated by Ficoll-hypaque gradient separation followed by overnight plastic adherence. Cultured monocytes (1 × 106ml) were stimulated with LPS (1 μg/ml) of either P. gingivalis or F. nucleatum for 2, 8, 24 and 48 h and supernatant fluids were collected. IL-1β, IL-6, and TNF-α levels in supernatant fluids were estimated by ELISA. Increased overall production of IL-1β, IL-6 and TNF-α by LPS stimulated monocytes isolated from HIV-1 infected patients was observed when compared to HIV-1 uninfected controls. LPS stimulated monocytes from HIV-1 infected patients with high viral load (HVL) produced significant (p |
| doi_str_mv | 10.3109/08923970009026002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_infor</sourceid><recordid>TN_cdi_proquest_miscellaneous_17606313</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17606313</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-2597f671d0e57bde9e0067cc9c999675855c1bde96700ac5216f243696697fa3</originalsourceid><addsrcrecordid>eNp9kMFKAzEQhoMoWKsP4C0nL7qabNxsg16ktHahaqHF65JmszR1N9Eki-xL-C76IH0ms9RLET0NzPzfDPMBcIrRJcGIXaEBiwlLEUIMxRSheA_0cELiKE4w2Qe9bh6FADkER86tEcIhnPTAx0ivuBaygJn20layeVE6wpuvi50GhVwXcNHUxsJHKaxxysExF97YaPMJZ9YUjfDKaLhs4XQ2h3Ov6qbiPiyeNDXX8MFoI1ovHcyc2Q5Ka2o4yZ7hOZxxr6T27hgclLxy8uSn9sFiPFoMJ9H06T4b3k0jQVLmw1MsLWmKCySTdFlIJhGiqRBMMMZomgySROCuT4MRLpIY0zK-JpRRGkBO-uBsu_bVmrdGOp_XyglZVVxL07gcpxRRgkkI4m2we9lZWeavVtXctjlGeSc-_yU-MLdbRunS2Jq_G1sVuedtZWxpg2zlOvRv_GYHX0le-ZXgVuZr01gdtPxz_BvDN5yD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17606313</pqid></control><display><type>article</type><title>Enhanced Interleukin-1β, Interleukin-6 and Tumor Necrosis Factor-α Production by LPS Stimulated Human Monocytes Isolated from HIV + Patients</title><source>Taylor & Francis Medical Library - CRKN</source><source>Taylor & Francis Journals Complete</source><creator>Baqui, A. A. M. A. ; Jabra-Rizk, Mary Ann ; Kelley, Jacqueline I. ; Zhang, Ming ; Falkler, William A. ; Meiller, Timothy F.</creator><creatorcontrib>Baqui, A. A. M. A. ; Jabra-Rizk, Mary Ann ; Kelley, Jacqueline I. ; Zhang, Ming ; Falkler, William A. ; Meiller, Timothy F.</creatorcontrib><description>Abstract
Periodontal disease and tooth loss is a common finding among advanced HIV+ patients. In addition to local oral lipopolysaccharide (LPS) stimulation, systemic up-regulation of monocyte pro-inflammatory cytokine secretion may also be involved in the pathogenesis of HIV disease. A study was undertaken to investigate IL-1β, IL-6 and TNF-α production by resting and LPS stimulated monocytes isolated from HIV + patients and also to investigate the relationship of the patient's HIV viral load status to the cytokine production. Whole blood samples in EDTA were collected from 39 HIV-1 infected patients and 20 age and sex matched uninfected controls. Plasma was separated by centrifugation. Viral load was determined using a quantitative RT-PCR. Monocytes were isolated by Ficoll-hypaque gradient separation followed by overnight plastic adherence. Cultured monocytes (1 × 106ml) were stimulated with LPS (1 μg/ml) of either P. gingivalis or F. nucleatum for 2, 8, 24 and 48 h and supernatant fluids were collected. IL-1β, IL-6, and TNF-α levels in supernatant fluids were estimated by ELISA. Increased overall production of IL-1β, IL-6 and TNF-α by LPS stimulated monocytes isolated from HIV-1 infected patients was observed when compared to HIV-1 uninfected controls. LPS stimulated monocytes from HIV-1 infected patients with high viral load (HVL) produced significant (p<0.05) elevations in these pro-inflammatory cytokines when compared to HIV-1 uninfected controls. Both LPS of P. gingivalis and F. nucleatum produced a comparable cytokine production by monocytes after 8 h of stimulation. These data suggest that enhanced IL-1β, IL-6 and TNF-α is produced by monocytes/macrophages isolated from HVL HIV + patients and may be involved in the overall pathogenesis of HIV-1 infection.</description><identifier>ISSN: 0892-3973</identifier><identifier>EISSN: 1532-2513</identifier><identifier>DOI: 10.3109/08923970009026002</identifier><language>eng</language><publisher>Informa UK Ltd</publisher><subject>Human immunodeficiency virus ; Interleukin 1^b ; Interleukin 1b</subject><ispartof>Immunopharmacology and immunotoxicology, 2000-01, Vol.22 (3), p.401-421</ispartof><rights>2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-2597f671d0e57bde9e0067cc9c999675855c1bde96700ac5216f243696697fa3</citedby><cites>FETCH-LOGICAL-c379t-2597f671d0e57bde9e0067cc9c999675855c1bde96700ac5216f243696697fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/08923970009026002$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/08923970009026002$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids></links><search><creatorcontrib>Baqui, A. A. M. A.</creatorcontrib><creatorcontrib>Jabra-Rizk, Mary Ann</creatorcontrib><creatorcontrib>Kelley, Jacqueline I.</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><creatorcontrib>Falkler, William A.</creatorcontrib><creatorcontrib>Meiller, Timothy F.</creatorcontrib><title>Enhanced Interleukin-1β, Interleukin-6 and Tumor Necrosis Factor-α Production by LPS Stimulated Human Monocytes Isolated from HIV + Patients</title><title>Immunopharmacology and immunotoxicology</title><description>Abstract
Periodontal disease and tooth loss is a common finding among advanced HIV+ patients. In addition to local oral lipopolysaccharide (LPS) stimulation, systemic up-regulation of monocyte pro-inflammatory cytokine secretion may also be involved in the pathogenesis of HIV disease. A study was undertaken to investigate IL-1β, IL-6 and TNF-α production by resting and LPS stimulated monocytes isolated from HIV + patients and also to investigate the relationship of the patient's HIV viral load status to the cytokine production. Whole blood samples in EDTA were collected from 39 HIV-1 infected patients and 20 age and sex matched uninfected controls. Plasma was separated by centrifugation. Viral load was determined using a quantitative RT-PCR. Monocytes were isolated by Ficoll-hypaque gradient separation followed by overnight plastic adherence. Cultured monocytes (1 × 106ml) were stimulated with LPS (1 μg/ml) of either P. gingivalis or F. nucleatum for 2, 8, 24 and 48 h and supernatant fluids were collected. IL-1β, IL-6, and TNF-α levels in supernatant fluids were estimated by ELISA. Increased overall production of IL-1β, IL-6 and TNF-α by LPS stimulated monocytes isolated from HIV-1 infected patients was observed when compared to HIV-1 uninfected controls. LPS stimulated monocytes from HIV-1 infected patients with high viral load (HVL) produced significant (p<0.05) elevations in these pro-inflammatory cytokines when compared to HIV-1 uninfected controls. Both LPS of P. gingivalis and F. nucleatum produced a comparable cytokine production by monocytes after 8 h of stimulation. These data suggest that enhanced IL-1β, IL-6 and TNF-α is produced by monocytes/macrophages isolated from HVL HIV + patients and may be involved in the overall pathogenesis of HIV-1 infection.</description><subject>Human immunodeficiency virus</subject><subject>Interleukin 1^b</subject><subject>Interleukin 1b</subject><issn>0892-3973</issn><issn>1532-2513</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp9kMFKAzEQhoMoWKsP4C0nL7qabNxsg16ktHahaqHF65JmszR1N9Eki-xL-C76IH0ms9RLET0NzPzfDPMBcIrRJcGIXaEBiwlLEUIMxRSheA_0cELiKE4w2Qe9bh6FADkER86tEcIhnPTAx0ivuBaygJn20layeVE6wpuvi50GhVwXcNHUxsJHKaxxysExF97YaPMJZ9YUjfDKaLhs4XQ2h3Ov6qbiPiyeNDXX8MFoI1ovHcyc2Q5Ka2o4yZ7hOZxxr6T27hgclLxy8uSn9sFiPFoMJ9H06T4b3k0jQVLmw1MsLWmKCySTdFlIJhGiqRBMMMZomgySROCuT4MRLpIY0zK-JpRRGkBO-uBsu_bVmrdGOp_XyglZVVxL07gcpxRRgkkI4m2we9lZWeavVtXctjlGeSc-_yU-MLdbRunS2Jq_G1sVuedtZWxpg2zlOvRv_GYHX0le-ZXgVuZr01gdtPxz_BvDN5yD</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Baqui, A. A. M. A.</creator><creator>Jabra-Rizk, Mary Ann</creator><creator>Kelley, Jacqueline I.</creator><creator>Zhang, Ming</creator><creator>Falkler, William A.</creator><creator>Meiller, Timothy F.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20000101</creationdate><title>Enhanced Interleukin-1β, Interleukin-6 and Tumor Necrosis Factor-α Production by LPS Stimulated Human Monocytes Isolated from HIV + Patients</title><author>Baqui, A. A. M. A. ; Jabra-Rizk, Mary Ann ; Kelley, Jacqueline I. ; Zhang, Ming ; Falkler, William A. ; Meiller, Timothy F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-2597f671d0e57bde9e0067cc9c999675855c1bde96700ac5216f243696697fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Human immunodeficiency virus</topic><topic>Interleukin 1^b</topic><topic>Interleukin 1b</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baqui, A. A. M. A.</creatorcontrib><creatorcontrib>Jabra-Rizk, Mary Ann</creatorcontrib><creatorcontrib>Kelley, Jacqueline I.</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><creatorcontrib>Falkler, William A.</creatorcontrib><creatorcontrib>Meiller, Timothy F.</creatorcontrib><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Immunopharmacology and immunotoxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baqui, A. A. M. A.</au><au>Jabra-Rizk, Mary Ann</au><au>Kelley, Jacqueline I.</au><au>Zhang, Ming</au><au>Falkler, William A.</au><au>Meiller, Timothy F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Interleukin-1β, Interleukin-6 and Tumor Necrosis Factor-α Production by LPS Stimulated Human Monocytes Isolated from HIV + Patients</atitle><jtitle>Immunopharmacology and immunotoxicology</jtitle><date>2000-01-01</date><risdate>2000</risdate><volume>22</volume><issue>3</issue><spage>401</spage><epage>421</epage><pages>401-421</pages><issn>0892-3973</issn><eissn>1532-2513</eissn><abstract>Abstract
Periodontal disease and tooth loss is a common finding among advanced HIV+ patients. In addition to local oral lipopolysaccharide (LPS) stimulation, systemic up-regulation of monocyte pro-inflammatory cytokine secretion may also be involved in the pathogenesis of HIV disease. A study was undertaken to investigate IL-1β, IL-6 and TNF-α production by resting and LPS stimulated monocytes isolated from HIV + patients and also to investigate the relationship of the patient's HIV viral load status to the cytokine production. Whole blood samples in EDTA were collected from 39 HIV-1 infected patients and 20 age and sex matched uninfected controls. Plasma was separated by centrifugation. Viral load was determined using a quantitative RT-PCR. Monocytes were isolated by Ficoll-hypaque gradient separation followed by overnight plastic adherence. Cultured monocytes (1 × 106ml) were stimulated with LPS (1 μg/ml) of either P. gingivalis or F. nucleatum for 2, 8, 24 and 48 h and supernatant fluids were collected. IL-1β, IL-6, and TNF-α levels in supernatant fluids were estimated by ELISA. Increased overall production of IL-1β, IL-6 and TNF-α by LPS stimulated monocytes isolated from HIV-1 infected patients was observed when compared to HIV-1 uninfected controls. LPS stimulated monocytes from HIV-1 infected patients with high viral load (HVL) produced significant (p<0.05) elevations in these pro-inflammatory cytokines when compared to HIV-1 uninfected controls. Both LPS of P. gingivalis and F. nucleatum produced a comparable cytokine production by monocytes after 8 h of stimulation. These data suggest that enhanced IL-1β, IL-6 and TNF-α is produced by monocytes/macrophages isolated from HVL HIV + patients and may be involved in the overall pathogenesis of HIV-1 infection.</abstract><pub>Informa UK Ltd</pub><doi>10.3109/08923970009026002</doi><tpages>21</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0892-3973 |
| ispartof | Immunopharmacology and immunotoxicology, 2000-01, Vol.22 (3), p.401-421 |
| issn | 0892-3973 1532-2513 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17606313 |
| source | Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Human immunodeficiency virus Interleukin 1^b Interleukin 1b |
| title | Enhanced Interleukin-1β, Interleukin-6 and Tumor Necrosis Factor-α Production by LPS Stimulated Human Monocytes Isolated from HIV + Patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T13%3A22%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_infor&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhanced%20Interleukin-1%CE%B2,%20Interleukin-6%20and%20Tumor%20Necrosis%20Factor-%CE%B1%20Production%20by%20LPS%20Stimulated%20Human%20Monocytes%20Isolated%20from%20HIV%20+%20Patients&rft.jtitle=Immunopharmacology%20and%20immunotoxicology&rft.au=Baqui,%20A.%20A.%20M.%20A.&rft.date=2000-01-01&rft.volume=22&rft.issue=3&rft.spage=401&rft.epage=421&rft.pages=401-421&rft.issn=0892-3973&rft.eissn=1532-2513&rft_id=info:doi/10.3109/08923970009026002&rft_dat=%3Cproquest_infor%3E17606313%3C/proquest_infor%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17606313&rft_id=info:pmid/&rfr_iscdi=true |