protocol for in vitro germination and sustainable growth of two tropical mistletoes
We report a protocol for in vitro germination and sustainable growth of two tropical mistletoes, Dendrophthoe pentandra and Macrosolen cochinchinensis. Normal mistletoe shoots with haustoria have been produced using Murashige and Skoog (MS) media supplemented with coconut water (15-20% v/v) in venti...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2005-02, Vol.80 (2), p.221-228 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Ang, S.L.P Youn, J.W.H |
| description | We report a protocol for in vitro germination and sustainable growth of two tropical mistletoes, Dendrophthoe pentandra and Macrosolen cochinchinensis. Normal mistletoe shoots with haustoria have been produced using Murashige and Skoog (MS) media supplemented with coconut water (15-20% v/v) in ventilated culture vessels. Germination of mistletoes took place on hormone-free basal MS media, and the time needed for germination was much shorter for Macrosolen than for Dendrophthoe. After germination, further development of the mistletoes required a hormonal input from the medium. We found that 20 micromolar 6-benzylaminopurine (BA) did allow normal development of only Dendrophthoe, while coconut water (15-20% v/v) gave complete development of both species in ventilated culture vessels. Multiplication of these mistletoes is also possible via the callus stage using half-strength MS media supplemented with BA at various concentrations in ventilated culture vessels. This is probably the first report of a successful culture for the mistletoe genus of Macrosolen. Compared to Macrosolen, the nutritional and hormonal requirements for Dendrophthoe are less demanding, making the latter a useful model for further mistletoe research. |
| doi_str_mv | 10.1007/s11240-004-9372-9 |
| format | Article |
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Normal mistletoe shoots with haustoria have been produced using Murashige and Skoog (MS) media supplemented with coconut water (15-20% v/v) in ventilated culture vessels. Germination of mistletoes took place on hormone-free basal MS media, and the time needed for germination was much shorter for Macrosolen than for Dendrophthoe. After germination, further development of the mistletoes required a hormonal input from the medium. We found that 20 micromolar 6-benzylaminopurine (BA) did allow normal development of only Dendrophthoe, while coconut water (15-20% v/v) gave complete development of both species in ventilated culture vessels. Multiplication of these mistletoes is also possible via the callus stage using half-strength MS media supplemented with BA at various concentrations in ventilated culture vessels. This is probably the first report of a successful culture for the mistletoe genus of Macrosolen. Compared to Macrosolen, the nutritional and hormonal requirements for Dendrophthoe are less demanding, making the latter a useful model for further mistletoe research.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-004-9372-9</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>benzyladenine ; Biological and medical sciences ; Biotechnology ; callus ; cell growth ; coconut milk ; culture media ; Dendrophthoe ; Dendrophthoe pentandra ; dose response ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; germination ; in vitro culture ; in vitro regeneration ; Loranthaceae ; Macrosolen cochinchinensis ; medicinal plants ; methodology ; Methods. Procedures. Technologies ; micropropagation ; Miscellaneous ; parasitic plants ; Plant cells and fungal cells ; shoots</subject><ispartof>Plant cell, tissue and organ culture, 2005-02, Vol.80 (2), p.221-228</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-e3926cba7895e82cfe8986e837b7ccfa4556271ba7dddcd6cd44c195bc5ca5ae3</citedby><cites>FETCH-LOGICAL-c330t-e3926cba7895e82cfe8986e837b7ccfa4556271ba7dddcd6cd44c195bc5ca5ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16446342$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Ang, S.L.P</creatorcontrib><creatorcontrib>Youn, J.W.H</creatorcontrib><title>protocol for in vitro germination and sustainable growth of two tropical mistletoes</title><title>Plant cell, tissue and organ culture</title><description>We report a protocol for in vitro germination and sustainable growth of two tropical mistletoes, Dendrophthoe pentandra and Macrosolen cochinchinensis. Normal mistletoe shoots with haustoria have been produced using Murashige and Skoog (MS) media supplemented with coconut water (15-20% v/v) in ventilated culture vessels. Germination of mistletoes took place on hormone-free basal MS media, and the time needed for germination was much shorter for Macrosolen than for Dendrophthoe. After germination, further development of the mistletoes required a hormonal input from the medium. We found that 20 micromolar 6-benzylaminopurine (BA) did allow normal development of only Dendrophthoe, while coconut water (15-20% v/v) gave complete development of both species in ventilated culture vessels. Multiplication of these mistletoes is also possible via the callus stage using half-strength MS media supplemented with BA at various concentrations in ventilated culture vessels. This is probably the first report of a successful culture for the mistletoe genus of Macrosolen. Compared to Macrosolen, the nutritional and hormonal requirements for Dendrophthoe are less demanding, making the latter a useful model for further mistletoe research.</description><subject>benzyladenine</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>callus</subject><subject>cell growth</subject><subject>coconut milk</subject><subject>culture media</subject><subject>Dendrophthoe</subject><subject>Dendrophthoe pentandra</subject><subject>dose response</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>germination</subject><subject>in vitro culture</subject><subject>in vitro regeneration</subject><subject>Loranthaceae</subject><subject>Macrosolen cochinchinensis</subject><subject>medicinal plants</subject><subject>methodology</subject><subject>Methods. Procedures. Technologies</subject><subject>micropropagation</subject><subject>Miscellaneous</subject><subject>parasitic plants</subject><subject>Plant cells and fungal cells</subject><subject>shoots</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpFkE1LAzEQhoMoWKs_wJO56C062XzuUYpfUPBQew7ZbLaubDc1SS3-e1Na8DIDwzPDvA9C1xTuKYB6SJRWHAgAJzVTFalP0IQKxYgAzk_RBKhURGqhztFFSl8AIBmnE7TYxJCDCwPuQsT9iH_6HANe-bjuR5v7MGI7tjhtU7Zl0Awer2LY5U8cOpx3ARd60zs74HWf8uBz8OkSnXV2SP7q2Kdo-fz0MXsl8_eXt9njnDjGIBPP6kq6xipdC68r13lda-k1U41yrrNcCFkpWoC2bV0rXcu5o7VonHBWWM-m6O5wt2T43vqUTfnB-WGwow_bZKiSICvQBaQH0MWQUvSd2cR-beOvoWD2-sxBnyn6zF5fKVN0ezxuU4nXRTu6Pv0vSs6LwapwNweus8HYVSzMclEBZQC1EkJr9gfb9HtE</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Ang, S.L.P</creator><creator>Youn, J.W.H</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050201</creationdate><title>protocol for in vitro germination and sustainable growth of two tropical mistletoes</title><author>Ang, S.L.P ; Youn, J.W.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-e3926cba7895e82cfe8986e837b7ccfa4556271ba7dddcd6cd44c195bc5ca5ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>benzyladenine</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>callus</topic><topic>cell growth</topic><topic>coconut milk</topic><topic>culture media</topic><topic>Dendrophthoe</topic><topic>Dendrophthoe pentandra</topic><topic>dose response</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>germination</topic><topic>in vitro culture</topic><topic>in vitro regeneration</topic><topic>Loranthaceae</topic><topic>Macrosolen cochinchinensis</topic><topic>medicinal plants</topic><topic>methodology</topic><topic>Methods. Procedures. Technologies</topic><topic>micropropagation</topic><topic>Miscellaneous</topic><topic>parasitic plants</topic><topic>Plant cells and fungal cells</topic><topic>shoots</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ang, S.L.P</creatorcontrib><creatorcontrib>Youn, J.W.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ang, S.L.P</au><au>Youn, J.W.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>protocol for in vitro germination and sustainable growth of two tropical mistletoes</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><date>2005-02-01</date><risdate>2005</risdate><volume>80</volume><issue>2</issue><spage>221</spage><epage>228</epage><pages>221-228</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>We report a protocol for in vitro germination and sustainable growth of two tropical mistletoes, Dendrophthoe pentandra and Macrosolen cochinchinensis. Normal mistletoe shoots with haustoria have been produced using Murashige and Skoog (MS) media supplemented with coconut water (15-20% v/v) in ventilated culture vessels. Germination of mistletoes took place on hormone-free basal MS media, and the time needed for germination was much shorter for Macrosolen than for Dendrophthoe. After germination, further development of the mistletoes required a hormonal input from the medium. We found that 20 micromolar 6-benzylaminopurine (BA) did allow normal development of only Dendrophthoe, while coconut water (15-20% v/v) gave complete development of both species in ventilated culture vessels. Multiplication of these mistletoes is also possible via the callus stage using half-strength MS media supplemented with BA at various concentrations in ventilated culture vessels. This is probably the first report of a successful culture for the mistletoe genus of Macrosolen. Compared to Macrosolen, the nutritional and hormonal requirements for Dendrophthoe are less demanding, making the latter a useful model for further mistletoe research.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/s11240-004-9372-9</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2005-02, Vol.80 (2), p.221-228 |
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| subjects | benzyladenine Biological and medical sciences Biotechnology callus cell growth coconut milk culture media Dendrophthoe Dendrophthoe pentandra dose response Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology germination in vitro culture in vitro regeneration Loranthaceae Macrosolen cochinchinensis medicinal plants methodology Methods. Procedures. Technologies micropropagation Miscellaneous parasitic plants Plant cells and fungal cells shoots |
| title | protocol for in vitro germination and sustainable growth of two tropical mistletoes |
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