Exocytosis of a complement component C3-like protein by tunicate hemocytes
This study investigates the exocytic responses of invertebrate hemocytes to pathogen-associated antigens. It demonstrates that a homologue of complement component C3, a key defensive protein of the innate immune system, is expressed by phagocytic hemocytes (non-refractile vacuolated cells) of the tu...
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| Veröffentlicht in: | Developmental and comparative immunology 2004-03, Vol.28 (3), p.181-190 |
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| container_title | Developmental and comparative immunology |
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| creator | Raftos, David A Fabbro, Megan Nair, Sham V |
| description | This study investigates the exocytic responses of invertebrate hemocytes to pathogen-associated antigens. It demonstrates that a homologue of complement component C3, a key defensive protein of the innate immune system, is expressed by phagocytic hemocytes (non-refractile vacuolated cells) of the tunicate,
Styela plicata. C3-like molecules are localized in sub-cellular vesicles and are rapidly exocytosed after stimulation with bacterial, fungal or algal cell surface molecules. Signal transduction analysis indicated that the induced secretion of C3-like molecules is mediated by a G-protein dependent signaling pathway, which modulates tubulin microtubules. All of this evidence indicates that hemocytes can contribute to host defense responses by rapidly exocytosing C3-like proteins at sites of infection. |
| doi_str_mv | 10.1016/S0145-305X(03)00136-8 |
| format | Article |
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Styela plicata. C3-like molecules are localized in sub-cellular vesicles and are rapidly exocytosed after stimulation with bacterial, fungal or algal cell surface molecules. Signal transduction analysis indicated that the induced secretion of C3-like molecules is mediated by a G-protein dependent signaling pathway, which modulates tubulin microtubules. All of this evidence indicates that hemocytes can contribute to host defense responses by rapidly exocytosing C3-like proteins at sites of infection.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/S0145-305X(03)00136-8</identifier><identifier>PMID: 14642885</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Alkaloids ; Animals ; Ascidiacea ; Ascidian ; Benzophenanthridines ; Blotting, Western ; Calcimycin - pharmacology ; Carrageenan - pharmacology ; Cell Survival - drug effects ; Cholera Toxin - pharmacology ; Colchicine - pharmacology ; Colforsin - pharmacology ; Complement ; Complement C3 - analysis ; Complement C3 - metabolism ; Complement C3 - secretion ; Cytochalasin D - pharmacology ; Cytoplasmic Vesicles - chemistry ; Enzyme-Linked Immunosorbent Assay ; Exocytosis ; Exocytosis - drug effects ; Exocytosis - physiology ; Hemocytes - chemistry ; Hemocytes - physiology ; Hemocytes - ultrastructure ; Immunohistochemistry ; Kinetics ; Lipopolysaccharides - pharmacology ; Mannans - pharmacology ; Marine ; Microscopy, Electron ; Phenanthridines - pharmacology ; Signal transduction ; Staurosporine - pharmacology ; Styela plicata ; Tetradecanoylphorbol Acetate - pharmacology ; Thapsigargin - pharmacology ; Tunicate ; Urochordata - cytology ; Urochordata - immunology ; Urochordata - physiology</subject><ispartof>Developmental and comparative immunology, 2004-03, Vol.28 (3), p.181-190</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-44b8f89f318cf9cf364c0d79bb84b72bc413ff603a9beecbfcdc6bf17b97a3913</citedby><cites>FETCH-LOGICAL-c392t-44b8f89f318cf9cf364c0d79bb84b72bc413ff603a9beecbfcdc6bf17b97a3913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0145-305X(03)00136-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14642885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raftos, David A</creatorcontrib><creatorcontrib>Fabbro, Megan</creatorcontrib><creatorcontrib>Nair, Sham V</creatorcontrib><title>Exocytosis of a complement component C3-like protein by tunicate hemocytes</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>This study investigates the exocytic responses of invertebrate hemocytes to pathogen-associated antigens. It demonstrates that a homologue of complement component C3, a key defensive protein of the innate immune system, is expressed by phagocytic hemocytes (non-refractile vacuolated cells) of the tunicate,
Styela plicata. C3-like molecules are localized in sub-cellular vesicles and are rapidly exocytosed after stimulation with bacterial, fungal or algal cell surface molecules. Signal transduction analysis indicated that the induced secretion of C3-like molecules is mediated by a G-protein dependent signaling pathway, which modulates tubulin microtubules. All of this evidence indicates that hemocytes can contribute to host defense responses by rapidly exocytosing C3-like proteins at sites of infection.</description><subject>Alkaloids</subject><subject>Animals</subject><subject>Ascidiacea</subject><subject>Ascidian</subject><subject>Benzophenanthridines</subject><subject>Blotting, Western</subject><subject>Calcimycin - pharmacology</subject><subject>Carrageenan - pharmacology</subject><subject>Cell Survival - drug effects</subject><subject>Cholera Toxin - pharmacology</subject><subject>Colchicine - pharmacology</subject><subject>Colforsin - pharmacology</subject><subject>Complement</subject><subject>Complement C3 - analysis</subject><subject>Complement C3 - metabolism</subject><subject>Complement C3 - secretion</subject><subject>Cytochalasin D - pharmacology</subject><subject>Cytoplasmic Vesicles - chemistry</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Exocytosis</subject><subject>Exocytosis - drug effects</subject><subject>Exocytosis - physiology</subject><subject>Hemocytes - chemistry</subject><subject>Hemocytes - physiology</subject><subject>Hemocytes - ultrastructure</subject><subject>Immunohistochemistry</subject><subject>Kinetics</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Mannans - pharmacology</subject><subject>Marine</subject><subject>Microscopy, Electron</subject><subject>Phenanthridines - pharmacology</subject><subject>Signal transduction</subject><subject>Staurosporine - pharmacology</subject><subject>Styela plicata</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Thapsigargin - pharmacology</subject><subject>Tunicate</subject><subject>Urochordata - cytology</subject><subject>Urochordata - immunology</subject><subject>Urochordata - physiology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAQgC0EoqXwE0CZEAyBc-0k9oRQVV6qxABIbFbsnIUhiUucIPrvSdoKRm65G757fYQcU7igQNPLJ6A8iRkkr2fAzgEoS2OxQ8ZUZDIGEHKXjH-RETkI4R36EBT2yYjylE-FSMbkYf7tzar1wYXI2yiPjK-WJVZYt-vS10M1Y3HpPjBaNr5FV0d6FbVd7UzeYvSG1TABwyHZs3kZ8GibJ-TlZv48u4sXj7f3s-tFbJictjHnWlghLaPCWGksS7mBIpNaC66zqTacMmtTYLnUiEZbU5hUW5ppmeVMUjYhp5u5_TWfHYZWVS4YLMu8Rt8FRbMUgCe8B5MNaBofQoNWLRtX5c1KUVCDRLWWqAZDCphaS1Si7zvZLuh0hcVf19ZaD1xtAOzf_HLYqGAc1gYL16BpVeHdPyt-AIIIgtk</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Raftos, David A</creator><creator>Fabbro, Megan</creator><creator>Nair, Sham V</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20040301</creationdate><title>Exocytosis of a complement component C3-like protein by tunicate hemocytes</title><author>Raftos, David A ; Fabbro, Megan ; Nair, Sham V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-44b8f89f318cf9cf364c0d79bb84b72bc413ff603a9beecbfcdc6bf17b97a3913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alkaloids</topic><topic>Animals</topic><topic>Ascidiacea</topic><topic>Ascidian</topic><topic>Benzophenanthridines</topic><topic>Blotting, Western</topic><topic>Calcimycin - pharmacology</topic><topic>Carrageenan - pharmacology</topic><topic>Cell Survival - drug effects</topic><topic>Cholera Toxin - pharmacology</topic><topic>Colchicine - pharmacology</topic><topic>Colforsin - pharmacology</topic><topic>Complement</topic><topic>Complement C3 - analysis</topic><topic>Complement C3 - metabolism</topic><topic>Complement C3 - secretion</topic><topic>Cytochalasin D - pharmacology</topic><topic>Cytoplasmic Vesicles - chemistry</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Exocytosis</topic><topic>Exocytosis - drug effects</topic><topic>Exocytosis - physiology</topic><topic>Hemocytes - chemistry</topic><topic>Hemocytes - physiology</topic><topic>Hemocytes - ultrastructure</topic><topic>Immunohistochemistry</topic><topic>Kinetics</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Mannans - pharmacology</topic><topic>Marine</topic><topic>Microscopy, Electron</topic><topic>Phenanthridines - pharmacology</topic><topic>Signal transduction</topic><topic>Staurosporine - pharmacology</topic><topic>Styela plicata</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Thapsigargin - pharmacology</topic><topic>Tunicate</topic><topic>Urochordata - cytology</topic><topic>Urochordata - immunology</topic><topic>Urochordata - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raftos, David A</creatorcontrib><creatorcontrib>Fabbro, Megan</creatorcontrib><creatorcontrib>Nair, Sham V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raftos, David A</au><au>Fabbro, Megan</au><au>Nair, Sham V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exocytosis of a complement component C3-like protein by tunicate hemocytes</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>28</volume><issue>3</issue><spage>181</spage><epage>190</epage><pages>181-190</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>This study investigates the exocytic responses of invertebrate hemocytes to pathogen-associated antigens. It demonstrates that a homologue of complement component C3, a key defensive protein of the innate immune system, is expressed by phagocytic hemocytes (non-refractile vacuolated cells) of the tunicate,
Styela plicata. C3-like molecules are localized in sub-cellular vesicles and are rapidly exocytosed after stimulation with bacterial, fungal or algal cell surface molecules. Signal transduction analysis indicated that the induced secretion of C3-like molecules is mediated by a G-protein dependent signaling pathway, which modulates tubulin microtubules. All of this evidence indicates that hemocytes can contribute to host defense responses by rapidly exocytosing C3-like proteins at sites of infection.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>14642885</pmid><doi>10.1016/S0145-305X(03)00136-8</doi><tpages>10</tpages></addata></record> |
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| subjects | Alkaloids Animals Ascidiacea Ascidian Benzophenanthridines Blotting, Western Calcimycin - pharmacology Carrageenan - pharmacology Cell Survival - drug effects Cholera Toxin - pharmacology Colchicine - pharmacology Colforsin - pharmacology Complement Complement C3 - analysis Complement C3 - metabolism Complement C3 - secretion Cytochalasin D - pharmacology Cytoplasmic Vesicles - chemistry Enzyme-Linked Immunosorbent Assay Exocytosis Exocytosis - drug effects Exocytosis - physiology Hemocytes - chemistry Hemocytes - physiology Hemocytes - ultrastructure Immunohistochemistry Kinetics Lipopolysaccharides - pharmacology Mannans - pharmacology Marine Microscopy, Electron Phenanthridines - pharmacology Signal transduction Staurosporine - pharmacology Styela plicata Tetradecanoylphorbol Acetate - pharmacology Thapsigargin - pharmacology Tunicate Urochordata - cytology Urochordata - immunology Urochordata - physiology |
| title | Exocytosis of a complement component C3-like protein by tunicate hemocytes |
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