Quantification of white spot syndrome virus DNA through a competitive polymerase chain reaction

A competitive polymerase chain reaction (PCR) method for quantification of white spot syndrome virus (WSSV) genome was developed. A pair of WSSV primers, designated WSSV341F/R, was selected to amplify a 341-bp DNA fragment from the WSSV genome. For a competitive internal standard, we constructed and...

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Veröffentlicht in:	Aquaculture 2000-09, Vol.189 (1), p.11-21
Hauptverfasser:	Tang, Kathy F.J., Lightner, Donald V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal aquaculture Animal productions Aquaculture Biological and medical sciences Competitive PCR Crustacea Decapoda Deoxyribonucleic acid DNA Fish Fundamental and applied biological sciences. Psychology Invertebrate aquaculture Microbiology Penaeid shrimp Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Virology Viruses White spot syndrome virus
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