Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene

A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agen...

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Veröffentlicht in:Plant Biotechnology 2002, Vol.19(4), pp.251-260
Hauptverfasser: SEKI, Hikaru, NAKAMURA, Naoki, MARUTANI, Mizuri, OKABE, Takeharu, SANEMATSU, Shiroh, INAGAKI, Yoshishige, TOYODA, Kazuhiro, SHIRAISHI, Tomonori, YAMADA, Tetsuji, ICHINOSE, Yuki
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Sprache:eng
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