Synergistic effects of hydrogen peroxide and ethanol on cell viability loss in PC12 cells by increase in mitochondrial permeability transition
The promoting effect of ethanol against the cytotoxicity of hydrogen peroxide (H 2O 2) in differentiated PC12 cells was assessed by measuring the effect on the mitochondrial membrane permeability. Treatment of PC12 cells with H 2O 2 resulted in the nuclear damage, decrease in the mitochondrial trans...
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| Veröffentlicht in: | Biochemical pharmacology 2005-07, Vol.70 (2), p.317-325 |
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| creator | Lee, Chung Soo Kim, Yun Jeong Ko, Hyun Hee Han, Eun Sook |
| description | The promoting effect of ethanol against the cytotoxicity of hydrogen peroxide (H
2O
2) in differentiated PC12 cells was assessed by measuring the effect on the mitochondrial membrane permeability. Treatment of PC12 cells with H
2O
2 resulted in the nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome
c, activation of caspase-3, increase in the formation of reactive oxygen species (ROS) and depletion of GSH. In PC12 cells and dopaminergic neuroblastoma SH-SY5Y cells, the promoting effect of ethanol on the H
2O
2-induced cell death was increased with exposure time. Ethanol promoted the nuclear damage, change in the mitochondrial membrane permeability, ROS formation and decrease in GSH contents due to H
2O
2 in PC12 cells. Catalase, carboxy-PTIO, Mn-TBAP,
N-acetylcysteine, cyclosporin A and trifluoperazine inhibited the H
2O
2 and ethanol-induced mitochondrial dysfunction and cell injury. The results show that the ethanol treatment promotes the cytotoxicity of H
2O
2 against PC12 cells. Ethanol may enhance the H
2O
2-induced viability loss in PC12 cells by promoting the mitochondrial membrane permeability change, release of cytochrome
c and subsequent activation of caspase-3, which is associated with the increased formation of ROS and depletion of GSH. The findings suggest that ethanol as a promoting agent for the formation of mitochondrial permeability transition may enhance the neuronal cell injury caused by oxidants. |
| doi_str_mv | 10.1016/j.bcp.2005.04.029 |
| format | Article |
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2O
2) in differentiated PC12 cells was assessed by measuring the effect on the mitochondrial membrane permeability. Treatment of PC12 cells with H
2O
2 resulted in the nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome
c, activation of caspase-3, increase in the formation of reactive oxygen species (ROS) and depletion of GSH. In PC12 cells and dopaminergic neuroblastoma SH-SY5Y cells, the promoting effect of ethanol on the H
2O
2-induced cell death was increased with exposure time. Ethanol promoted the nuclear damage, change in the mitochondrial membrane permeability, ROS formation and decrease in GSH contents due to H
2O
2 in PC12 cells. Catalase, carboxy-PTIO, Mn-TBAP,
N-acetylcysteine, cyclosporin A and trifluoperazine inhibited the H
2O
2 and ethanol-induced mitochondrial dysfunction and cell injury. The results show that the ethanol treatment promotes the cytotoxicity of H
2O
2 against PC12 cells. Ethanol may enhance the H
2O
2-induced viability loss in PC12 cells by promoting the mitochondrial membrane permeability change, release of cytochrome
c and subsequent activation of caspase-3, which is associated with the increased formation of ROS and depletion of GSH. The findings suggest that ethanol as a promoting agent for the formation of mitochondrial permeability transition may enhance the neuronal cell injury caused by oxidants.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/j.bcp.2005.04.029</identifier><identifier>PMID: 15927145</identifier><identifier>CODEN: BCPCA6</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell injury ; Cell Survival - drug effects ; Cell Survival - physiology ; Dose-Response Relationship, Drug ; Drug Synergism ; Ethanol ; Ethanol - pharmacology ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Intracellular Membranes - drug effects ; Intracellular Membranes - physiology ; Medical sciences ; Membrane Potentials - physiology ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mitochondrial membrane permeability ; PC12 Cells ; Permeability - drug effects ; Pharmacology. Drug treatments ; Rats</subject><ispartof>Biochemical pharmacology, 2005-07, Vol.70 (2), p.317-325</ispartof><rights>2005 Elsevier Inc.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-f86811e73541a0150fc579beda67caa21ca7acbaad62f2932b150459af6e0ef63</citedby><cites>FETCH-LOGICAL-c478t-f86811e73541a0150fc579beda67caa21ca7acbaad62f2932b150459af6e0ef63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bcp.2005.04.029$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16907516$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15927145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Chung Soo</creatorcontrib><creatorcontrib>Kim, Yun Jeong</creatorcontrib><creatorcontrib>Ko, Hyun Hee</creatorcontrib><creatorcontrib>Han, Eun Sook</creatorcontrib><title>Synergistic effects of hydrogen peroxide and ethanol on cell viability loss in PC12 cells by increase in mitochondrial permeability transition</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>The promoting effect of ethanol against the cytotoxicity of hydrogen peroxide (H
2O
2) in differentiated PC12 cells was assessed by measuring the effect on the mitochondrial membrane permeability. Treatment of PC12 cells with H
2O
2 resulted in the nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome
c, activation of caspase-3, increase in the formation of reactive oxygen species (ROS) and depletion of GSH. In PC12 cells and dopaminergic neuroblastoma SH-SY5Y cells, the promoting effect of ethanol on the H
2O
2-induced cell death was increased with exposure time. Ethanol promoted the nuclear damage, change in the mitochondrial membrane permeability, ROS formation and decrease in GSH contents due to H
2O
2 in PC12 cells. Catalase, carboxy-PTIO, Mn-TBAP,
N-acetylcysteine, cyclosporin A and trifluoperazine inhibited the H
2O
2 and ethanol-induced mitochondrial dysfunction and cell injury. The results show that the ethanol treatment promotes the cytotoxicity of H
2O
2 against PC12 cells. Ethanol may enhance the H
2O
2-induced viability loss in PC12 cells by promoting the mitochondrial membrane permeability change, release of cytochrome
c and subsequent activation of caspase-3, which is associated with the increased formation of ROS and depletion of GSH. The findings suggest that ethanol as a promoting agent for the formation of mitochondrial permeability transition may enhance the neuronal cell injury caused by oxidants.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell injury</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Synergism</subject><subject>Ethanol</subject><subject>Ethanol - pharmacology</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Intracellular Membranes - drug effects</subject><subject>Intracellular Membranes - physiology</subject><subject>Medical sciences</subject><subject>Membrane Potentials - physiology</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondrial membrane permeability</subject><subject>PC12 Cells</subject><subject>Permeability - drug effects</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO1DAQRS0EYpqBD2CDvIFdB9uJ7USsUGt4SCOBBKytilOediuxGzs9Ij_BN-PQjWbHyo86dXWrLiEvOas44-rtoertsRKMyYo1FRPdI7Lhra63olPtY7JhjKlyl-KKPMv5sD5bxZ-SKy47oXkjN-T3tyVguvN59paic2jnTKOj-2VI8Q4DPWKKv_yAFMJAcd5DiCONgVocR3rvofejnxc6xpypD_Trjou_tUz7pXzYhJBxrUx-jnYfw5A8jKvshP-a5wQh-9nH8Jw8cTBmfHE5r8mPDzffd5-2t18-ft69v93aRrfz1rWq5Rx1LRsOjEvmrNRdjwMobQEEt6DB9gCDEk50tegL08gOnEKGTtXX5M1Z95jizxPm2Uw-r7YhYDxlw7XsmKrbAvIzaFOZMKEzx-QnSIvhzKwhmIMpIZg1BMMaU0IoPa8u4qd-wuGh47L1Ary-AJAtjK6Mb31-4FTHtOSry3dnDssq7j0mk63HYHHwqQRlhuj_Y-MPuCinoA</recordid><startdate>20050715</startdate><enddate>20050715</enddate><creator>Lee, Chung Soo</creator><creator>Kim, Yun Jeong</creator><creator>Ko, Hyun Hee</creator><creator>Han, Eun Sook</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20050715</creationdate><title>Synergistic effects of hydrogen peroxide and ethanol on cell viability loss in PC12 cells by increase in mitochondrial permeability transition</title><author>Lee, Chung Soo ; Kim, Yun Jeong ; Ko, Hyun Hee ; Han, Eun Sook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-f86811e73541a0150fc579beda67caa21ca7acbaad62f2932b150459af6e0ef63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell injury</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - physiology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Synergism</topic><topic>Ethanol</topic><topic>Ethanol - pharmacology</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Intracellular Membranes - drug effects</topic><topic>Intracellular Membranes - physiology</topic><topic>Medical sciences</topic><topic>Membrane Potentials - physiology</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondrial membrane permeability</topic><topic>PC12 Cells</topic><topic>Permeability - drug effects</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Chung Soo</creatorcontrib><creatorcontrib>Kim, Yun Jeong</creatorcontrib><creatorcontrib>Ko, Hyun Hee</creatorcontrib><creatorcontrib>Han, Eun Sook</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Chung Soo</au><au>Kim, Yun Jeong</au><au>Ko, Hyun Hee</au><au>Han, Eun Sook</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergistic effects of hydrogen peroxide and ethanol on cell viability loss in PC12 cells by increase in mitochondrial permeability transition</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>2005-07-15</date><risdate>2005</risdate><volume>70</volume><issue>2</issue><spage>317</spage><epage>325</epage><pages>317-325</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><coden>BCPCA6</coden><abstract>The promoting effect of ethanol against the cytotoxicity of hydrogen peroxide (H
2O
2) in differentiated PC12 cells was assessed by measuring the effect on the mitochondrial membrane permeability. Treatment of PC12 cells with H
2O
2 resulted in the nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome
c, activation of caspase-3, increase in the formation of reactive oxygen species (ROS) and depletion of GSH. In PC12 cells and dopaminergic neuroblastoma SH-SY5Y cells, the promoting effect of ethanol on the H
2O
2-induced cell death was increased with exposure time. Ethanol promoted the nuclear damage, change in the mitochondrial membrane permeability, ROS formation and decrease in GSH contents due to H
2O
2 in PC12 cells. Catalase, carboxy-PTIO, Mn-TBAP,
N-acetylcysteine, cyclosporin A and trifluoperazine inhibited the H
2O
2 and ethanol-induced mitochondrial dysfunction and cell injury. The results show that the ethanol treatment promotes the cytotoxicity of H
2O
2 against PC12 cells. Ethanol may enhance the H
2O
2-induced viability loss in PC12 cells by promoting the mitochondrial membrane permeability change, release of cytochrome
c and subsequent activation of caspase-3, which is associated with the increased formation of ROS and depletion of GSH. The findings suggest that ethanol as a promoting agent for the formation of mitochondrial permeability transition may enhance the neuronal cell injury caused by oxidants.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15927145</pmid><doi>10.1016/j.bcp.2005.04.029</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cell injury Cell Survival - drug effects Cell Survival - physiology Dose-Response Relationship, Drug Drug Synergism Ethanol Ethanol - pharmacology Hydrogen peroxide Hydrogen Peroxide - pharmacology Intracellular Membranes - drug effects Intracellular Membranes - physiology Medical sciences Membrane Potentials - physiology Mitochondria - drug effects Mitochondria - metabolism Mitochondrial membrane permeability PC12 Cells Permeability - drug effects Pharmacology. Drug treatments Rats |
| title | Synergistic effects of hydrogen peroxide and ethanol on cell viability loss in PC12 cells by increase in mitochondrial permeability transition |
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