ISOLATION AND CHARACTERIZATION OF SIX cDNAs INVOLVED IN CARBON METABOLISM IN LAMINARIA DIGITATA (PHAEOPHYCEAE)
An expressed sequence tag (EST) approach was used to retrieve cDNA clones involved in carbon metabolism in Laminaria digitata Lamouroux. Six partial open reading frames were identified, respectively encoding an α-type carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluco...
Gespeichert in:
| Veröffentlicht in: | Journal of phycology 1999-12, Vol.35 (6), p.1237-1245 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1245 |
|---|---|
| container_issue | 6 |
| container_start_page | 1237 |
| container_title | Journal of phycology |
| container_volume | 35 |
| creator | Moulin, Pascale Crépineau, Florent Kloareg, Bernard Boyen, Catherine |
| description | An expressed sequence tag (EST) approach was used to retrieve cDNA clones involved in carbon metabolism in Laminaria digitata Lamouroux. Six partial open reading frames were identified, respectively encoding an α-type carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), phosphoglycerate kinase (PGK), glycolate oxidase (GLO), and GDP-4-keto-6-d-mannose-3,5-epimerase-4-reductase, also known as fucose synthase (FS). These enzymes were further characterized through Southern blot analyses, amino acid sequence comparisons, and patterns of expression. In contrast to the other genes, which are expressed in both the gametophytic and sporophytic generations of L. digitata, the α-type carbonic anhydrase messenger RNA was shown by RT-PCR and northern blot analyses to be present in the gametophytes only. The evolutionary relationships of these genes and their interest as molecular tools for investigating carbon fluxes in brown algae are discussed. |
| doi_str_mv | 10.1046/j.1529-8817.1999.3561237.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17584627</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17584627</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4177-8f30aac053ac1ec62c30e5a3ffa574d229ebda1ac31c081936eeb765fa8fed143</originalsourceid><addsrcrecordid>eNqVkFFP2zAUhS20SXRsv4FoD9P2kGDHiZ3szSRp6ylNShvY2MuVcZ0ppVAWgyj_fo6CeJ9kyb7nnnMkfwh9JjggOGJn24DEYeonCeEBSdM0oDEjIeXB4QhN3lbv0ATjMPQpi9gx-mDtFmPMWUwm6F6u61I0sq48UeVeNhcrkTXFSv4exXrqreUvT-eVsJ6sruryqsjdw8vE6tztF0UjzutSrheDWIqFrMRKCi-XM9mIRnhfl3NR1Mv5dVaI4ttH9L5VO2s-vd4n6HJaNNncL-uZzETp64hw7ictxUppHFOlidEs1BSbWNG2VTGPNmGYmpuNIkpTonFCUsqMuXEfalXSmg2J6An6MvY-9Pu_T8Y-wl1ntdnt1L3ZP1kgPE4iFnJn_D4adb-3tjctPPTdnepfgGAYEMMWBo4wcIQBMbwihoMLT8fwc7czL_-RhB_La3fcDIPgivyxqLOP5vBWpPpbYJzyGH5WM7jIedNEFYbc-U9Hf6v2oP70nYXLdYgJxY4FJpjSf3oZlHE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17584627</pqid></control><display><type>article</type><title>ISOLATION AND CHARACTERIZATION OF SIX cDNAs INVOLVED IN CARBON METABOLISM IN LAMINARIA DIGITATA (PHAEOPHYCEAE)</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Moulin, Pascale ; Crépineau, Florent ; Kloareg, Bernard ; Boyen, Catherine</creator><creatorcontrib>Moulin, Pascale ; Crépineau, Florent ; Kloareg, Bernard ; Boyen, Catherine</creatorcontrib><description>An expressed sequence tag (EST) approach was used to retrieve cDNA clones involved in carbon metabolism in Laminaria digitata Lamouroux. Six partial open reading frames were identified, respectively encoding an α-type carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), phosphoglycerate kinase (PGK), glycolate oxidase (GLO), and GDP-4-keto-6-d-mannose-3,5-epimerase-4-reductase, also known as fucose synthase (FS). These enzymes were further characterized through Southern blot analyses, amino acid sequence comparisons, and patterns of expression. In contrast to the other genes, which are expressed in both the gametophytic and sporophytic generations of L. digitata, the α-type carbonic anhydrase messenger RNA was shown by RT-PCR and northern blot analyses to be present in the gametophytes only. The evolutionary relationships of these genes and their interest as molecular tools for investigating carbon fluxes in brown algae are discussed.</description><identifier>ISSN: 0022-3646</identifier><identifier>EISSN: 1529-8817</identifier><identifier>DOI: 10.1046/j.1529-8817.1999.3561237.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Carbon ; carbon metabolism ; Carbonic anhydrase ; differential expression ; expressed sequence tags ; Fucose synthase ; gametophyte ; Laminaria digitata ; Marine ; Phaeophyceae ; Phosphogluconate 2-dehydrogenase ; phosphogluconate dehydrogenase ; sporophyte</subject><ispartof>Journal of phycology, 1999-12, Vol.35 (6), p.1237-1245</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4177-8f30aac053ac1ec62c30e5a3ffa574d229ebda1ac31c081936eeb765fa8fed143</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1529-8817.1999.3561237.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1529-8817.1999.3561237.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids></links><search><creatorcontrib>Moulin, Pascale</creatorcontrib><creatorcontrib>Crépineau, Florent</creatorcontrib><creatorcontrib>Kloareg, Bernard</creatorcontrib><creatorcontrib>Boyen, Catherine</creatorcontrib><title>ISOLATION AND CHARACTERIZATION OF SIX cDNAs INVOLVED IN CARBON METABOLISM IN LAMINARIA DIGITATA (PHAEOPHYCEAE)</title><title>Journal of phycology</title><description>An expressed sequence tag (EST) approach was used to retrieve cDNA clones involved in carbon metabolism in Laminaria digitata Lamouroux. Six partial open reading frames were identified, respectively encoding an α-type carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), phosphoglycerate kinase (PGK), glycolate oxidase (GLO), and GDP-4-keto-6-d-mannose-3,5-epimerase-4-reductase, also known as fucose synthase (FS). These enzymes were further characterized through Southern blot analyses, amino acid sequence comparisons, and patterns of expression. In contrast to the other genes, which are expressed in both the gametophytic and sporophytic generations of L. digitata, the α-type carbonic anhydrase messenger RNA was shown by RT-PCR and northern blot analyses to be present in the gametophytes only. The evolutionary relationships of these genes and their interest as molecular tools for investigating carbon fluxes in brown algae are discussed.</description><subject>Carbon</subject><subject>carbon metabolism</subject><subject>Carbonic anhydrase</subject><subject>differential expression</subject><subject>expressed sequence tags</subject><subject>Fucose synthase</subject><subject>gametophyte</subject><subject>Laminaria digitata</subject><subject>Marine</subject><subject>Phaeophyceae</subject><subject>Phosphogluconate 2-dehydrogenase</subject><subject>phosphogluconate dehydrogenase</subject><subject>sporophyte</subject><issn>0022-3646</issn><issn>1529-8817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqVkFFP2zAUhS20SXRsv4FoD9P2kGDHiZ3szSRp6ylNShvY2MuVcZ0ppVAWgyj_fo6CeJ9kyb7nnnMkfwh9JjggOGJn24DEYeonCeEBSdM0oDEjIeXB4QhN3lbv0ATjMPQpi9gx-mDtFmPMWUwm6F6u61I0sq48UeVeNhcrkTXFSv4exXrqreUvT-eVsJ6sruryqsjdw8vE6tztF0UjzutSrheDWIqFrMRKCi-XM9mIRnhfl3NR1Mv5dVaI4ttH9L5VO2s-vd4n6HJaNNncL-uZzETp64hw7ictxUppHFOlidEs1BSbWNG2VTGPNmGYmpuNIkpTonFCUsqMuXEfalXSmg2J6An6MvY-9Pu_T8Y-wl1ntdnt1L3ZP1kgPE4iFnJn_D4adb-3tjctPPTdnepfgGAYEMMWBo4wcIQBMbwihoMLT8fwc7czL_-RhB_La3fcDIPgivyxqLOP5vBWpPpbYJzyGH5WM7jIedNEFYbc-U9Hf6v2oP70nYXLdYgJxY4FJpjSf3oZlHE</recordid><startdate>199912</startdate><enddate>199912</enddate><creator>Moulin, Pascale</creator><creator>Crépineau, Florent</creator><creator>Kloareg, Bernard</creator><creator>Boyen, Catherine</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>199912</creationdate><title>ISOLATION AND CHARACTERIZATION OF SIX cDNAs INVOLVED IN CARBON METABOLISM IN LAMINARIA DIGITATA (PHAEOPHYCEAE)</title><author>Moulin, Pascale ; Crépineau, Florent ; Kloareg, Bernard ; Boyen, Catherine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4177-8f30aac053ac1ec62c30e5a3ffa574d229ebda1ac31c081936eeb765fa8fed143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Carbon</topic><topic>carbon metabolism</topic><topic>Carbonic anhydrase</topic><topic>differential expression</topic><topic>expressed sequence tags</topic><topic>Fucose synthase</topic><topic>gametophyte</topic><topic>Laminaria digitata</topic><topic>Marine</topic><topic>Phaeophyceae</topic><topic>Phosphogluconate 2-dehydrogenase</topic><topic>phosphogluconate dehydrogenase</topic><topic>sporophyte</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moulin, Pascale</creatorcontrib><creatorcontrib>Crépineau, Florent</creatorcontrib><creatorcontrib>Kloareg, Bernard</creatorcontrib><creatorcontrib>Boyen, Catherine</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moulin, Pascale</au><au>Crépineau, Florent</au><au>Kloareg, Bernard</au><au>Boyen, Catherine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ISOLATION AND CHARACTERIZATION OF SIX cDNAs INVOLVED IN CARBON METABOLISM IN LAMINARIA DIGITATA (PHAEOPHYCEAE)</atitle><jtitle>Journal of phycology</jtitle><date>1999-12</date><risdate>1999</risdate><volume>35</volume><issue>6</issue><spage>1237</spage><epage>1245</epage><pages>1237-1245</pages><issn>0022-3646</issn><eissn>1529-8817</eissn><abstract>An expressed sequence tag (EST) approach was used to retrieve cDNA clones involved in carbon metabolism in Laminaria digitata Lamouroux. Six partial open reading frames were identified, respectively encoding an α-type carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), phosphoglycerate kinase (PGK), glycolate oxidase (GLO), and GDP-4-keto-6-d-mannose-3,5-epimerase-4-reductase, also known as fucose synthase (FS). These enzymes were further characterized through Southern blot analyses, amino acid sequence comparisons, and patterns of expression. In contrast to the other genes, which are expressed in both the gametophytic and sporophytic generations of L. digitata, the α-type carbonic anhydrase messenger RNA was shown by RT-PCR and northern blot analyses to be present in the gametophytes only. The evolutionary relationships of these genes and their interest as molecular tools for investigating carbon fluxes in brown algae are discussed.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1046/j.1529-8817.1999.3561237.x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3646 |
| ispartof | Journal of phycology, 1999-12, Vol.35 (6), p.1237-1245 |
| issn | 0022-3646 1529-8817 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17584627 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Carbon carbon metabolism Carbonic anhydrase differential expression expressed sequence tags Fucose synthase gametophyte Laminaria digitata Marine Phaeophyceae Phosphogluconate 2-dehydrogenase phosphogluconate dehydrogenase sporophyte |
| title | ISOLATION AND CHARACTERIZATION OF SIX cDNAs INVOLVED IN CARBON METABOLISM IN LAMINARIA DIGITATA (PHAEOPHYCEAE) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T07%3A53%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=ISOLATION%20AND%20CHARACTERIZATION%20OF%20SIX%20cDNAs%20INVOLVED%20IN%20CARBON%20METABOLISM%20IN%20LAMINARIA%20DIGITATA%20(PHAEOPHYCEAE)&rft.jtitle=Journal%20of%20phycology&rft.au=Moulin,%20Pascale&rft.date=1999-12&rft.volume=35&rft.issue=6&rft.spage=1237&rft.epage=1245&rft.pages=1237-1245&rft.issn=0022-3646&rft.eissn=1529-8817&rft_id=info:doi/10.1046/j.1529-8817.1999.3561237.x&rft_dat=%3Cproquest_cross%3E17584627%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17584627&rft_id=info:pmid/&rfr_iscdi=true |