Ischemia induces metallothionein III expression in neurons of rat brain
Metallothionein III (MT-III) is a brain-specific member of the metallothionein family and binds zinc in vivo. In order to confirm the precise localization of MT-III in normal rat brain and the change of MT-III expression after transient whole brain ischemia, we raised a high affinity phagemid-antibo...
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| Veröffentlicht in: | Life sciences (1973) 1999, Vol.64 (8), p.707-715 |
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| container_title | Life sciences (1973) |
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| creator | Yanagitani, Shingo Miyazaki, Hiroaki Nakahashi, Yoshitsugu Kuno, Kenji Ueno, Yohji Matsushita, Masanori Naitoh, Yuji Taketani, Shigeru Inoue, Kyoichi |
| description | Metallothionein III (MT-III) is a brain-specific member of the metallothionein family and binds zinc in vivo. In order to confirm the precise localization of MT-III in normal rat brain and the change of MT-III expression after transient whole brain ischemia, we raised a high affinity phagemid-antibody specific for rat MT-III. Immunohistochemical analysis revealed that MT-III in normal brain is localized abundantly in neuronal cell bodies in CA1-3 regions of hippocampus, dentate gyrus, cerebral cortex, olfactory bulb and Purkinje cells in cerebellum. This expression pattern of MT-III was similar to that of MT-III mRNA observed by
in situ hybridization studies. ELISA and Northern blot analysis revealed that MT-III protein as well as mRNA levels were up-regulated in cerebrum soon after ischemic stress. Immunohistochemical analysis also demonstrated intense staining in neurons in injured brain after ischemia, which distributed in the same regions as in normal brain. These results suggest that MT-III plays an important role in protecting neurons from ischemic insult by reducing neurotoxic zinc levels and inhibits uncontrolled growth of neurites after ischemia. |
| doi_str_mv | 10.1016/S0024-3205(98)00612-2 |
| format | Article |
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in situ hybridization studies. ELISA and Northern blot analysis revealed that MT-III protein as well as mRNA levels were up-regulated in cerebrum soon after ischemic stress. Immunohistochemical analysis also demonstrated intense staining in neurons in injured brain after ischemia, which distributed in the same regions as in normal brain. These results suggest that MT-III plays an important role in protecting neurons from ischemic insult by reducing neurotoxic zinc levels and inhibits uncontrolled growth of neurites after ischemia.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/S0024-3205(98)00612-2</identifier><identifier>PMID: 10069533</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Antibodies, Monoclonal ; Blotting, Northern ; Brain - metabolism ; Brain - pathology ; Cloning, Molecular ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Genetic Vectors ; Immunohistochemistry ; ischemia ; Ischemic Attack, Transient - metabolism ; Male ; metallothionein III ; Nerve Tissue Proteins - biosynthesis ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - immunology ; Nerve Tissue Proteins - isolation & purification ; Neurons - chemistry ; Neurons - metabolism ; Northern blot ; phagemid-antibody ; Rats ; Rats, Wistar ; Recombinant Fusion Proteins - immunology ; Recombinant Fusion Proteins - isolation & purification ; RNA, Messenger - metabolism ; Time Factors ; Up-Regulation ; Western blot ; zinc</subject><ispartof>Life sciences (1973), 1999, Vol.64 (8), p.707-715</ispartof><rights>1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-5f5fc031e3fd15aca6351acb722010620cf71c7c1a8f767d024ec6b23a95dc7c3</citedby><cites>FETCH-LOGICAL-c392t-5f5fc031e3fd15aca6351acb722010620cf71c7c1a8f767d024ec6b23a95dc7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0024-3205(98)00612-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,4026,27930,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10069533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yanagitani, Shingo</creatorcontrib><creatorcontrib>Miyazaki, Hiroaki</creatorcontrib><creatorcontrib>Nakahashi, Yoshitsugu</creatorcontrib><creatorcontrib>Kuno, Kenji</creatorcontrib><creatorcontrib>Ueno, Yohji</creatorcontrib><creatorcontrib>Matsushita, Masanori</creatorcontrib><creatorcontrib>Naitoh, Yuji</creatorcontrib><creatorcontrib>Taketani, Shigeru</creatorcontrib><creatorcontrib>Inoue, Kyoichi</creatorcontrib><title>Ischemia induces metallothionein III expression in neurons of rat brain</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Metallothionein III (MT-III) is a brain-specific member of the metallothionein family and binds zinc in vivo. In order to confirm the precise localization of MT-III in normal rat brain and the change of MT-III expression after transient whole brain ischemia, we raised a high affinity phagemid-antibody specific for rat MT-III. Immunohistochemical analysis revealed that MT-III in normal brain is localized abundantly in neuronal cell bodies in CA1-3 regions of hippocampus, dentate gyrus, cerebral cortex, olfactory bulb and Purkinje cells in cerebellum. This expression pattern of MT-III was similar to that of MT-III mRNA observed by
in situ hybridization studies. ELISA and Northern blot analysis revealed that MT-III protein as well as mRNA levels were up-regulated in cerebrum soon after ischemic stress. Immunohistochemical analysis also demonstrated intense staining in neurons in injured brain after ischemia, which distributed in the same regions as in normal brain. These results suggest that MT-III plays an important role in protecting neurons from ischemic insult by reducing neurotoxic zinc levels and inhibits uncontrolled growth of neurites after ischemia.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Blotting, Northern</subject><subject>Brain - metabolism</subject><subject>Brain - pathology</subject><subject>Cloning, Molecular</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Genetic Vectors</subject><subject>Immunohistochemistry</subject><subject>ischemia</subject><subject>Ischemic Attack, Transient - metabolism</subject><subject>Male</subject><subject>metallothionein III</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - immunology</subject><subject>Nerve Tissue Proteins - isolation & purification</subject><subject>Neurons - chemistry</subject><subject>Neurons - metabolism</subject><subject>Northern blot</subject><subject>phagemid-antibody</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><subject>Up-Regulation</subject><subject>Western blot</subject><subject>zinc</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7rr6E5ScRA_VfJhmexJZdC0seFDPIU0nbKRt1qQV_fdmtyLePA0MzzvD-yB0SskVJTS_fiaE3WScEXFRzC8JySnL2B6a0rksMpJzuo-mv8gEHcX4RggRQvJDNKGJLwTnU7Qso1lD6zR2XT0YiLiFXjeN79fOd-A6XJYlhs9NgBjTJmG4gyH4LmJvcdA9roJ23TE6sLqJcPIzZ-j14f5l8Zitnpbl4m6VGV6wPhNWWEM4BW5rKrTRORdUm0oyRijJGTFWUiMN1XMrc1mnAmDyinFdiDrt-Qydj3c3wb8PEHvVumigaXQHfoiKSiHzVDSBYgRN8DEGsGoTXKvDl6JEbQ2qnUG11aOKudoZVCzlzn4eDFUL9Z_UqCwBtyMAqeaHg6CicdAZqF0A06vau39efAMpeIAB</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Yanagitani, Shingo</creator><creator>Miyazaki, Hiroaki</creator><creator>Nakahashi, Yoshitsugu</creator><creator>Kuno, Kenji</creator><creator>Ueno, Yohji</creator><creator>Matsushita, Masanori</creator><creator>Naitoh, Yuji</creator><creator>Taketani, Shigeru</creator><creator>Inoue, Kyoichi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>1999</creationdate><title>Ischemia induces metallothionein III expression in neurons of rat brain</title><author>Yanagitani, Shingo ; Miyazaki, Hiroaki ; Nakahashi, Yoshitsugu ; Kuno, Kenji ; Ueno, Yohji ; Matsushita, Masanori ; Naitoh, Yuji ; Taketani, Shigeru ; Inoue, Kyoichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-5f5fc031e3fd15aca6351acb722010620cf71c7c1a8f767d024ec6b23a95dc7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Blotting, Northern</topic><topic>Brain - metabolism</topic><topic>Brain - pathology</topic><topic>Cloning, Molecular</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Genetic Vectors</topic><topic>Immunohistochemistry</topic><topic>ischemia</topic><topic>Ischemic Attack, Transient - metabolism</topic><topic>Male</topic><topic>metallothionein III</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - immunology</topic><topic>Nerve Tissue Proteins - isolation & purification</topic><topic>Neurons - chemistry</topic><topic>Neurons - metabolism</topic><topic>Northern blot</topic><topic>phagemid-antibody</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><topic>Up-Regulation</topic><topic>Western blot</topic><topic>zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yanagitani, Shingo</creatorcontrib><creatorcontrib>Miyazaki, Hiroaki</creatorcontrib><creatorcontrib>Nakahashi, Yoshitsugu</creatorcontrib><creatorcontrib>Kuno, Kenji</creatorcontrib><creatorcontrib>Ueno, Yohji</creatorcontrib><creatorcontrib>Matsushita, Masanori</creatorcontrib><creatorcontrib>Naitoh, Yuji</creatorcontrib><creatorcontrib>Taketani, Shigeru</creatorcontrib><creatorcontrib>Inoue, Kyoichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yanagitani, Shingo</au><au>Miyazaki, Hiroaki</au><au>Nakahashi, Yoshitsugu</au><au>Kuno, Kenji</au><au>Ueno, Yohji</au><au>Matsushita, Masanori</au><au>Naitoh, Yuji</au><au>Taketani, Shigeru</au><au>Inoue, Kyoichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ischemia induces metallothionein III expression in neurons of rat brain</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1999</date><risdate>1999</risdate><volume>64</volume><issue>8</issue><spage>707</spage><epage>715</epage><pages>707-715</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Metallothionein III (MT-III) is a brain-specific member of the metallothionein family and binds zinc in vivo. In order to confirm the precise localization of MT-III in normal rat brain and the change of MT-III expression after transient whole brain ischemia, we raised a high affinity phagemid-antibody specific for rat MT-III. Immunohistochemical analysis revealed that MT-III in normal brain is localized abundantly in neuronal cell bodies in CA1-3 regions of hippocampus, dentate gyrus, cerebral cortex, olfactory bulb and Purkinje cells in cerebellum. This expression pattern of MT-III was similar to that of MT-III mRNA observed by
in situ hybridization studies. ELISA and Northern blot analysis revealed that MT-III protein as well as mRNA levels were up-regulated in cerebrum soon after ischemic stress. Immunohistochemical analysis also demonstrated intense staining in neurons in injured brain after ischemia, which distributed in the same regions as in normal brain. These results suggest that MT-III plays an important role in protecting neurons from ischemic insult by reducing neurotoxic zinc levels and inhibits uncontrolled growth of neurites after ischemia.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>10069533</pmid><doi>10.1016/S0024-3205(98)00612-2</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Antibodies, Monoclonal Blotting, Northern Brain - metabolism Brain - pathology Cloning, Molecular ELISA Enzyme-Linked Immunosorbent Assay Genetic Vectors Immunohistochemistry ischemia Ischemic Attack, Transient - metabolism Male metallothionein III Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - immunology Nerve Tissue Proteins - isolation & purification Neurons - chemistry Neurons - metabolism Northern blot phagemid-antibody Rats Rats, Wistar Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - isolation & purification RNA, Messenger - metabolism Time Factors Up-Regulation Western blot zinc |
| title | Ischemia induces metallothionein III expression in neurons of rat brain |
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