Germline chimeric chickens from FACS-sorted donor cells

Germline chimeric chickens can be constructed by injecting donor chicken blastodermal cells (CBCs) into recipient embryos and incubating to hatch. Transgenic chickens can be produced through chimeric intermediates if the donor cells are genetically manipulated; the chance of producing a transgenic c...

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Veröffentlicht in:	Molecular reproduction and development 1999, Vol.52 (1), p.33-42
Hauptverfasser:	Speksnijder, G.J, Etches, R.J, Gibbins, A.M.V
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-galactosidase Biological and medical sciences Biotechnology blastoderm chickens chimerism embryo (animal) fluorescence activated cell sorting Fundamental and applied biological sciences. Psychology gene transfer Genetic engineering genetic markers Genetic technics germ cells lacZ Methods. Procedures. Technologies sorting transfection transgenic Transgenic animals Transgenic animals and transgenic plants β-galactosidase
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