Expression in Escherichia coli of the IpdA gene, protein sequence analysis and immunological characterization of the p64k protein from Neisseria meningitidis
By making use of recombinant DNA technology it is possible to characterize meningococcal outer membrane proteins (OMPs) capable of stimulating a host immune response. The IpdA gene, which codes for an OMP (P64k) from Neisseria meningitidis, was cloned in Escherichia coli. The recombinant protein was...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 1998-06, Vol.27 (3), p.189-196 |
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| container_title | Biotechnology and applied biochemistry |
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| creator | GUILLEN, G ALVAREZ, A CARMENATE, T GONZALEZ, L. J ESTRADA, R TAMBARA, Y PADRON, G HERRERA, L SILVA, R MORERA, V GONZALEZ, S MUSACCHIO, A BESADA, V COIZEAU, E CABALLERO, E NAZABAL, C |
| description | By making use of recombinant DNA technology it is possible to characterize meningococcal outer membrane proteins (OMPs) capable of stimulating a host immune response. The IpdA gene, which codes for an OMP (P64k) from Neisseria meningitidis, was cloned in Escherichia coli. The recombinant protein was recognized by sera from patients convalescing from meningococcal disease. The monoclonal antibodies obtained against the recombinant protein recognized the natural protein on a Western blot, and monoclonal antibody 114 was assayed in ELISA with a panel of 85 N. meningitidis strains. The protein was recognized in 81 strains (95.3%); the strains that were not recognized were neither epidemic nor isolated from systemic disease. The complete amino acid sequence of P64k was obtained by automatic sequencing and MS. |
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J ; ESTRADA, R ; TAMBARA, Y ; PADRON, G ; HERRERA, L ; SILVA, R ; MORERA, V ; GONZALEZ, S ; MUSACCHIO, A ; BESADA, V ; COIZEAU, E ; CABALLERO, E ; NAZABAL, C</creator><creatorcontrib>GUILLEN, G ; ALVAREZ, A ; CARMENATE, T ; GONZALEZ, L. J ; ESTRADA, R ; TAMBARA, Y ; PADRON, G ; HERRERA, L ; SILVA, R ; MORERA, V ; GONZALEZ, S ; MUSACCHIO, A ; BESADA, V ; COIZEAU, E ; CABALLERO, E ; NAZABAL, C</creatorcontrib><description>By making use of recombinant DNA technology it is possible to characterize meningococcal outer membrane proteins (OMPs) capable of stimulating a host immune response. The IpdA gene, which codes for an OMP (P64k) from Neisseria meningitidis, was cloned in Escherichia coli. The recombinant protein was recognized by sera from patients convalescing from meningococcal disease. The monoclonal antibodies obtained against the recombinant protein recognized the natural protein on a Western blot, and monoclonal antibody 114 was assayed in ELISA with a panel of 85 N. meningitidis strains. The protein was recognized in 81 strains (95.3%); the strains that were not recognized were neither epidemic nor isolated from systemic disease. The complete amino acid sequence of P64k was obtained by automatic sequencing and MS.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>CODEN: BABIEC</identifier><language>eng</language><publisher>London: Portland Press</publisher><subject>Bacteriology ; Biological and medical sciences ; Escherichia coli ; Fundamental and applied biological sciences. 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The IpdA gene, which codes for an OMP (P64k) from Neisseria meningitidis, was cloned in Escherichia coli. The recombinant protein was recognized by sera from patients convalescing from meningococcal disease. The monoclonal antibodies obtained against the recombinant protein recognized the natural protein on a Western blot, and monoclonal antibody 114 was assayed in ELISA with a panel of 85 N. meningitidis strains. The protein was recognized in 81 strains (95.3%); the strains that were not recognized were neither epidemic nor isolated from systemic disease. The complete amino acid sequence of P64k was obtained by automatic sequencing and MS.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IpdA gene</subject><subject>Microbiology</subject><subject>Neisseria meningitidis</subject><subject>outer membrane proteins</subject><subject>P64k protein</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNo9j81Kw0AUhYMoWKvvMAtxZWAyP8lkWUrVQtGNrsN0cpNeTWbi3BSs7-K7mmJxde7i47vnnCWzTBU8NYVS58mMG6NTpTN5mVwRvXPOTWHELPlZfQ0RiDB4hp6tyO0gotuhZS50yELDxh2w9VAvWAse7tkQwwgTSvC5B--AWW-7AyFNR82w7_c-dKFFZzvmdjZaN07GbzseX5x0Q64-_kVNDD17BiSaOMt68OhbHLFGuk4uGtsR3Jxynrw9rF6XT-nm5XG9XGzSVmg-prUua6UKkZU1lIUp8lxkjdRCcS6kE1oJbYQA54DnzuZiq7eZ4VtVSq0cV1LOk7s_79RpWkVj1SM56DrrIeypygqdqzI7grcn0NI0sInWO6RqiNjbeKiE1NzoXP4CoTR1rA</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>GUILLEN, G</creator><creator>ALVAREZ, A</creator><creator>CARMENATE, T</creator><creator>GONZALEZ, L. 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Psychology</topic><topic>IpdA gene</topic><topic>Microbiology</topic><topic>Neisseria meningitidis</topic><topic>outer membrane proteins</topic><topic>P64k protein</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GUILLEN, G</creatorcontrib><creatorcontrib>ALVAREZ, A</creatorcontrib><creatorcontrib>CARMENATE, T</creatorcontrib><creatorcontrib>GONZALEZ, L. 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| ispartof | Biotechnology and applied biochemistry, 1998-06, Vol.27 (3), p.189-196 |
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| language | eng |
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| source | Wiley Online Library |
| subjects | Bacteriology Biological and medical sciences Escherichia coli Fundamental and applied biological sciences. Psychology IpdA gene Microbiology Neisseria meningitidis outer membrane proteins P64k protein Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies |
| title | Expression in Escherichia coli of the IpdA gene, protein sequence analysis and immunological characterization of the p64k protein from Neisseria meningitidis |
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