Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3

Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3

The dibenzothiophene (DBT) metabolic pathway in Mycobacterium strain G3, which is classified as a desulfurizing microorganism with the 4S pathway, was analyzed. 2-Hydroxybiphenyl (HBP), which is an end metabolite in the DBT desulfurization reaction, and 2-methoxybiphenyl (MBP) were found in the reac...

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Veröffentlicht in:Journal of bioscience and bioengineering 2002-01, Vol.93 (5), p.491-497
Hauptverfasser: Okada, Hideki, Nomura, Nobuhiko, Nakahara, Tadaatsu, Maruhashi, Kenji
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Mycobacterium
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container_end_page 497
container_issue 5
container_start_page 491
container_title Journal of bioscience and bioengineering
container_volume 93
creator Okada, Hideki
Nomura, Nobuhiko
Nakahara, Tadaatsu
Maruhashi, Kenji
description The dibenzothiophene (DBT) metabolic pathway in Mycobacterium strain G3, which is classified as a desulfurizing microorganism with the 4S pathway, was analyzed. 2-Hydroxybiphenyl (HBP), which is an end metabolite in the DBT desulfurization reaction, and 2-methoxybiphenyl (MBP) were found in the reaction mixture, and the methoxylation pathway from HBP to MBP was clarified. Although the substrate in the methoxylation reaction was HBP, there was no relationship between expression of the methoxylation activity and that of the desulfurization activity. Then, 4,6-dimethyl DBT, 4,6-diethyl DBT and benzo[b]naphtho[2,1- d]thiophene were metabolized to their methoxy forms via the desulfurization pathway. We established the methoxylation pathway in Mycobacterium G3.
doi_str_mv 10.1263/jbb.93.491
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title Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3
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