The Elp2 Subunit of Elongator and Elongating RNA Polymerase II Holoenzyme Is a WD40 Repeat Protein
A novel yeast gene, ELP2, is shown to encode the 90-kDa subunit of the Elongator complex and elongating RNA polymerase II holoenzyme. ELP2 encodes a protein with eight WD40 repeats, and cells lacking the gene display typicalelp phenotypes, such as temperature and salt sensitivity. Generally, differe...
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| Veröffentlicht in: | The Journal of biological chemistry 2000-04, Vol.275 (17), p.12896-12899 |
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| container_end_page | 12899 |
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| container_issue | 17 |
| container_start_page | 12896 |
| container_title | The Journal of biological chemistry |
| container_volume | 275 |
| creator | Fellows, Jane Erdjument-Bromage, Hediye Tempst, Paul Svejstrup, Jesper Q. |
| description | A novel yeast gene, ELP2, is shown to encode the 90-kDa subunit of the Elongator complex and elongating RNA polymerase II holoenzyme. ELP2 encodes a protein with eight WD40 repeats, and cells lacking the gene display typicalelp phenotypes, such as temperature and salt sensitivity. Generally, different combinations of double and triple ELPgene deletions cause the same phenotypes as single ELP1,ELP2, or ELP3 deletion, providing genetic evidence that the ELP gene products work together in a complex. |
| doi_str_mv | 10.1074/jbc.275.17.12896 |
| format | Article |
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ELP2 encodes a protein with eight WD40 repeats, and cells lacking the gene display typicalelp phenotypes, such as temperature and salt sensitivity. 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ELP2 encodes a protein with eight WD40 repeats, and cells lacking the gene display typicalelp phenotypes, such as temperature and salt sensitivity. Generally, different combinations of double and triple ELPgene deletions cause the same phenotypes as single ELP1,ELP2, or ELP3 deletion, providing genetic evidence that the ELP gene products work together in a complex.</description><subject>Amino Acid Sequence</subject><subject>binding proteins</subject><subject>biological resistance</subject><subject>Blotting, Western</subject><subject>deletions</subject><subject>DNA-directed RNA polymerase</subject><subject>ELP2 gene</subject><subject>Gene Deletion</subject><subject>Holoenzymes - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>mutants</subject><subject>Phenotype</subject><subject>Protein Binding</subject><subject>Repetitive Sequences, Amino Acid</subject><subject>RNA Polymerase II - chemistry</subject><subject>RNA Polymerase II - physiology</subject><subject>Saccharomyces cerevisiae</subject><subject>salinity</subject><subject>Sequence Homology, Amino Acid</subject><subject>sodium chloride</subject><subject>temperature</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtP3DAURq2qCAbKnhX1ArHL1K_EcXcjHp2RUDviobKzHOfOjFESD3YCor--LqESqlRvrGud79P1QeiIkiklUnx5qOyUyXxK5ZSyUhUf0ISSkmc8p_cf0YQQRjPF8nIP7cf4QNIRiu6ivRSWMi_LCapuN4Avmi3DN0M1dK7HfpVm361N7wM2Xf13ct0aX3-f4aVvXloIJgJeLPDcNx66X-kFLyI2-Oe5IPgatmB6vAy-B9d9Qjsr00Q4fLsP0N3lxe3ZPLv68W1xNrvKrJCsz4RQipaCWFnXhZG5lLYC4JRTCgWVlsuKQMEIE7VRooIy_VcZJQmXXBaC8wN0OvZug38cIPa6ddFC05gO_BA1lXnBGVMJJCNog48xwEpvg2tNeNGU6D9edfKqk9cU0a9eU-T4rXuoWqjfBUaRCTgZgY1bb55dAF05bzfQ_tvzecRWxmuzDi7quxtGKCdM5TxVJeLrSEBS9eQg6GgddBbqVGp7XXv3_zV_AzVzmXM</recordid><startdate>20000428</startdate><enddate>20000428</enddate><creator>Fellows, Jane</creator><creator>Erdjument-Bromage, Hediye</creator><creator>Tempst, Paul</creator><creator>Svejstrup, Jesper Q.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>20000428</creationdate><title>The Elp2 Subunit of Elongator and Elongating RNA Polymerase II Holoenzyme Is a WD40 Repeat Protein</title><author>Fellows, Jane ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Svejstrup, Jesper Q.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-44991840c7dd6a7577cbee31311e617c37b0e62024da94be88969a97037376433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>binding proteins</topic><topic>biological resistance</topic><topic>Blotting, Western</topic><topic>deletions</topic><topic>DNA-directed RNA polymerase</topic><topic>ELP2 gene</topic><topic>Gene Deletion</topic><topic>Holoenzymes - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>mutants</topic><topic>Phenotype</topic><topic>Protein Binding</topic><topic>Repetitive Sequences, Amino Acid</topic><topic>RNA Polymerase II - chemistry</topic><topic>RNA Polymerase II - physiology</topic><topic>Saccharomyces cerevisiae</topic><topic>salinity</topic><topic>Sequence Homology, Amino Acid</topic><topic>sodium chloride</topic><topic>temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fellows, Jane</creatorcontrib><creatorcontrib>Erdjument-Bromage, Hediye</creatorcontrib><creatorcontrib>Tempst, Paul</creatorcontrib><creatorcontrib>Svejstrup, Jesper Q.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fellows, Jane</au><au>Erdjument-Bromage, Hediye</au><au>Tempst, Paul</au><au>Svejstrup, Jesper Q.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Elp2 Subunit of Elongator and Elongating RNA Polymerase II Holoenzyme Is a WD40 Repeat Protein</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2000-04-28</date><risdate>2000</risdate><volume>275</volume><issue>17</issue><spage>12896</spage><epage>12899</epage><pages>12896-12899</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>A novel yeast gene, ELP2, is shown to encode the 90-kDa subunit of the Elongator complex and elongating RNA polymerase II holoenzyme. 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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 2000-04, Vol.275 (17), p.12896-12899 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence binding proteins biological resistance Blotting, Western deletions DNA-directed RNA polymerase ELP2 gene Gene Deletion Holoenzymes - chemistry Molecular Sequence Data Mutagenesis mutants Phenotype Protein Binding Repetitive Sequences, Amino Acid RNA Polymerase II - chemistry RNA Polymerase II - physiology Saccharomyces cerevisiae salinity Sequence Homology, Amino Acid sodium chloride temperature |
| title | The Elp2 Subunit of Elongator and Elongating RNA Polymerase II Holoenzyme Is a WD40 Repeat Protein |
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