Lack of a significant role for the PerR regulator in Bacillus subtilis spore resistance
Bacillus subtilis cells lacking the PerR repressor which regulates transcription of genes encoding oxidative stress protective proteins grew at 30–50% the rate of wild-type cells, and perR cultures accumulated rapidly growing suppressor mutants lacking the catalase whose expression is regulated by P...
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| Veröffentlicht in: | FEMS microbiology letters 2000-07, Vol.188 (2), p.203-208 |
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| creator | Casillas-Martinez, Lilliam Driks, Adam Setlow, Barbara Setlow, Peter |
| description | Bacillus subtilis cells lacking the PerR repressor which regulates transcription of genes encoding oxidative stress protective proteins grew at 30–50% the rate of wild-type cells, and
perR cultures accumulated rapidly growing suppressor mutants lacking the catalase whose expression is regulated by PerR. However,
perR spores which retained the
perR regulated catalase were obtained on plates. These
perR spores had levels of oxidative stress protective proteins from 7- to 50-fold higher than those in wild-type spores, but
perR spore resistance to heat, hydrogen peroxide and
t-butyl hydroperoxide was essentially identical to that of wild-type spores, indicating that elevated levels of proteins that protect growing cells from oxidizing agents play no role in dormant spore resistance to these compounds. However, germinated
perR spores were much more resistant to alkyl hydroperoxides than were wild-type spores. |
| doi_str_mv | 10.1016/S0378-1097(00)00236-6 |
| format | Article |
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perR cultures accumulated rapidly growing suppressor mutants lacking the catalase whose expression is regulated by PerR. However,
perR spores which retained the
perR regulated catalase were obtained on plates. These
perR spores had levels of oxidative stress protective proteins from 7- to 50-fold higher than those in wild-type spores, but
perR spore resistance to heat, hydrogen peroxide and
t-butyl hydroperoxide was essentially identical to that of wild-type spores, indicating that elevated levels of proteins that protect growing cells from oxidizing agents play no role in dormant spore resistance to these compounds. However, germinated
perR spores were much more resistant to alkyl hydroperoxides than were wild-type spores.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(00)00236-6</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Alkyl hydroperoxide reductase ; Bacillus subtilis ; Catalase ; hydrogen peroxide ; Oxidative stress ; PerR protein ; Spore resistance ; t-Butyl hydroperoxide</subject><ispartof>FEMS microbiology letters, 2000-07, Vol.188 (2), p.203-208</ispartof><rights>2000 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Casillas-Martinez, Lilliam</creatorcontrib><creatorcontrib>Driks, Adam</creatorcontrib><creatorcontrib>Setlow, Barbara</creatorcontrib><creatorcontrib>Setlow, Peter</creatorcontrib><title>Lack of a significant role for the PerR regulator in Bacillus subtilis spore resistance</title><title>FEMS microbiology letters</title><description>Bacillus subtilis cells lacking the PerR repressor which regulates transcription of genes encoding oxidative stress protective proteins grew at 30–50% the rate of wild-type cells, and
perR cultures accumulated rapidly growing suppressor mutants lacking the catalase whose expression is regulated by PerR. However,
perR spores which retained the
perR regulated catalase were obtained on plates. These
perR spores had levels of oxidative stress protective proteins from 7- to 50-fold higher than those in wild-type spores, but
perR spore resistance to heat, hydrogen peroxide and
t-butyl hydroperoxide was essentially identical to that of wild-type spores, indicating that elevated levels of proteins that protect growing cells from oxidizing agents play no role in dormant spore resistance to these compounds. However, germinated
perR spores were much more resistant to alkyl hydroperoxides than were wild-type spores.</description><subject>Alkyl hydroperoxide reductase</subject><subject>Bacillus subtilis</subject><subject>Catalase</subject><subject>hydrogen peroxide</subject><subject>Oxidative stress</subject><subject>PerR protein</subject><subject>Spore resistance</subject><subject>t-Butyl hydroperoxide</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNo9kEtLxDAUhYMoOI7-BCEr0UX1NmmTdCU6-IIBxQcuQ5LejNHYatL6--2ouDqXw8fl8BGyX8JxCaU4eQAuVVFCIw8BjgAYF4XYILOyllUhGqE2yewf2SY7Ob8CQMVAzMjz0rg32ntqaA6rLvjgTDfQ1Eekvk90eEF6h-meJlyN0QxTFTp6blyIccw0j3YIMUzHR59wgnLIg-kc7pItb2LGvb-ck6fLi8fFdbG8vbpZnC0LZMBEga1SjDPVVL71rZWSW4lgG66st7IV3qPyTe1qA17VtWKtdZXg1lbOWus5n5OD378fqf8cMQ_6PWSHMZoO-zHrUtY1U5OSOTn9BXFa8xUw6ewCTkvbkNANuu2DLkGvheofoXptSwPoH6Fa8G-JB2sh</recordid><startdate>20000715</startdate><enddate>20000715</enddate><creator>Casillas-Martinez, Lilliam</creator><creator>Driks, Adam</creator><creator>Setlow, Barbara</creator><creator>Setlow, Peter</creator><general>Elsevier B.V</general><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20000715</creationdate><title>Lack of a significant role for the PerR regulator in Bacillus subtilis spore resistance</title><author>Casillas-Martinez, Lilliam ; Driks, Adam ; Setlow, Barbara ; Setlow, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e2026-ed88232894fdfdb773b7e0b938bfb7d6ffe8f95c5a0f85582dbc463bb4cbbbf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alkyl hydroperoxide reductase</topic><topic>Bacillus subtilis</topic><topic>Catalase</topic><topic>hydrogen peroxide</topic><topic>Oxidative stress</topic><topic>PerR protein</topic><topic>Spore resistance</topic><topic>t-Butyl hydroperoxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casillas-Martinez, Lilliam</creatorcontrib><creatorcontrib>Driks, Adam</creatorcontrib><creatorcontrib>Setlow, Barbara</creatorcontrib><creatorcontrib>Setlow, Peter</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casillas-Martinez, Lilliam</au><au>Driks, Adam</au><au>Setlow, Barbara</au><au>Setlow, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of a significant role for the PerR regulator in Bacillus subtilis spore resistance</atitle><jtitle>FEMS microbiology letters</jtitle><date>2000-07-15</date><risdate>2000</risdate><volume>188</volume><issue>2</issue><spage>203</spage><epage>208</epage><pages>203-208</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Bacillus subtilis cells lacking the PerR repressor which regulates transcription of genes encoding oxidative stress protective proteins grew at 30–50% the rate of wild-type cells, and
perR cultures accumulated rapidly growing suppressor mutants lacking the catalase whose expression is regulated by PerR. However,
perR spores which retained the
perR regulated catalase were obtained on plates. These
perR spores had levels of oxidative stress protective proteins from 7- to 50-fold higher than those in wild-type spores, but
perR spore resistance to heat, hydrogen peroxide and
t-butyl hydroperoxide was essentially identical to that of wild-type spores, indicating that elevated levels of proteins that protect growing cells from oxidizing agents play no role in dormant spore resistance to these compounds. However, germinated
perR spores were much more resistant to alkyl hydroperoxides than were wild-type spores.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S0378-1097(00)00236-6</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2000-07, Vol.188 (2), p.203-208 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17552802 |
| source | Wiley Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Alkyl hydroperoxide reductase Bacillus subtilis Catalase hydrogen peroxide Oxidative stress PerR protein Spore resistance t-Butyl hydroperoxide |
| title | Lack of a significant role for the PerR regulator in Bacillus subtilis spore resistance |
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