The capacitating agent bicarbonate induces protein kinase A-dependent changes in phospholipid transbilayer behavior in the sperm plasma membrane

A flow cytometric procedure was used to follow the effect of bicarbonate, a key inducer of sperm capacitation in vitro, on the transbilayer behavior of C6NBD-phospholipids in the plasma membrane of living acrosome-intact boar spermatozoa under physiological conditions. In the absence of bicarbonate,...

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Veröffentlicht in:	Development (Cambridge) 2000-06, Vol.127 (11), p.2407-2420
Hauptverfasser:	Gadella, B M, Harrison, R A
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Chloro-7-nitrobenzofurazan - analogs & derivatives 4-Chloro-7-nitrobenzofurazan - metabolism Acrosome - metabolism Animals bicarbonate Bicarbonates - metabolism Bicarbonates - pharmacology Calcium - metabolism Cell Membrane - drug effects Cell Membrane - metabolism Cyclic AMP - metabolism Cyclic AMP-Dependent Protein Kinases - metabolism Dose-Response Relationship, Drug Fluorescent Dyes - metabolism Lipid Bilayers - metabolism Male Oxadiazoles - metabolism phosphatidylcholine Phosphatidylcholines - metabolism phosphatidylethanolamine Phosphatidylethanolamines - metabolism phosphatidylserine Phosphatidylserines - metabolism Phospholipids - metabolism Phosphorylation Pyrimidinones - metabolism Sperm Capacitation - physiology Spermatozoa - drug effects Spermatozoa - metabolism sphingomyelin Sphingomyelins - metabolism Swine
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