Ex vivo construction of a novel model of bioengineered bladder mucosa: A preliminary study

Objective To generate and to evaluate ex vivo a novel model of bioengineered human bladder mucosa based on fibrin‐agarose biomaterials. Methods We first established primary cultures of stromal and epithelial cells from small biopsies of the human bladder using enzymatic digestion and selective cell...

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Veröffentlicht in:	International journal of urology 2016-01, Vol.23 (1), p.85-92
Hauptverfasser:	Jaimes-Parra, Boris D, Valle-Díaz de la Guardia, Francisco, Arrabal-Polo, Miguel Á, Herrera-Imbroda, Bernardo, Lara, María F, Machuca-Santa-Cruz, Francisco-Javier, Campos, Antonio, Alaminos, Miguel, Crespo, Pascual V, Garzón, Ingrid
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Basement Membrane - ultrastructure Biocompatible Materials bladder mucosa Cell Survival cytokeratins Epithelial Cells Fibrin fibrin-agarose Humans Intermediate Filament Proteins - analysis Keratin-13 - analysis Keratin-4 - analysis Keratin-7 - analysis Keratin-8 - analysis Male Middle Aged Mucous Membrane - chemistry Mucous Membrane - cytology Mucous Membrane - ultrastructure Primary Cell Culture Sepharose Stromal Cells tissue engineering Tissue Engineering - methods Tissue Scaffolds Urinary Bladder - cytology Uroplakin III - analysis Zonula Occludens-2 Protein - analysis
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