Therapeutic effect of astragaloside-IV on bradycardia is involved in up-regulating klotho expression
In order to determine whether klotho is involved in the therapeutic effects of Astragaloside-IV on bradycardia, we evaluated the effect of ASG-IV on klotho and the effect of klotho on HCN4 and If. Administrating isoproterenol (5mg/kg) for 15days to establish a rat bradycardia model randomized SD rat...
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| Veröffentlicht in: | Life sciences (1973) 2016-01, Vol.144, p.94-102 |
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| creator | Qiu, Xuejia Guo, Qiao Xiong, Wei Yang, Xia Tang, Yi-qun |
| description | In order to determine whether klotho is involved in the therapeutic effects of Astragaloside-IV on bradycardia, we evaluated the effect of ASG-IV on klotho and the effect of klotho on HCN4 and If.
Administrating isoproterenol (5mg/kg) for 15days to establish a rat bradycardia model randomized SD rats into control, model (ISO) and ASG-IV (5mg/kg/day) groups to explore the effect of ASG-IV on klotho. Rats were sacrificed on day15 after heart rate and heart function were measured; SAN tissues were collected to measure the expression of klotho and HCN4. In vitro, neonatal rat myocardial cells were incubated with LPS for 24h to inhibit the expression of HCN4 and incubated with LPS+ klotho to explore the effect of klotho on HCN4 expression. We also adopted full-patch-clamp technique to explore the effect of klotho on If.
Heart rate in model group was significantly decreased (356.6±19.7 vs. 428.9±19.9 in control group, P |
| doi_str_mv | 10.1016/j.lfs.2015.11.021 |
| format | Article |
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Administrating isoproterenol (5mg/kg) for 15days to establish a rat bradycardia model randomized SD rats into control, model (ISO) and ASG-IV (5mg/kg/day) groups to explore the effect of ASG-IV on klotho. Rats were sacrificed on day15 after heart rate and heart function were measured; SAN tissues were collected to measure the expression of klotho and HCN4. In vitro, neonatal rat myocardial cells were incubated with LPS for 24h to inhibit the expression of HCN4 and incubated with LPS+ klotho to explore the effect of klotho on HCN4 expression. We also adopted full-patch-clamp technique to explore the effect of klotho on If.
Heart rate in model group was significantly decreased (356.6±19.7 vs. 428.9±19.9 in control group, P<0.01) and ASG-IV can increase heart rate (401.4±12.0 vs. 356.6±19.7 in model group, P<0.01). The expression of klotho was also up-regulated (P<0.05). In vitro, after incubation with LPS for 24h, HCN4 expression was significantly decreased in neonatal rat myocardial cells (0.6±0.07 vs. 1.0, P<0.01) and If was significantly declined. Exogenous klotho showed protective effect on HCN4 expression (1.58±0.16 in ASG-IV group vs. 0.6±0.07 in LPS group, P<0.05) and If.
Klotho is involved in the treatment mechanism of ASG-IV.
[Display omitted]</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2015.11.021</identifier><identifier>PMID: 26593401</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adrenergic beta-Agonists ; Animals ; Animals, Newborn ; Astragaloside IV ; Bradycardia ; Bradycardia - chemically induced ; Bradycardia - drug therapy ; Bradycardia - genetics ; Glucuronidase - biosynthesis ; Glucuronidase - genetics ; HCN4 ; Heart Function Tests ; Heart Rate - drug effects ; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - biosynthesis ; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - genetics ; ISO ; Isoproterenol ; Klotho ; Lipopolysaccharides - pharmacology ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; Patch-Clamp Techniques ; Potassium Channels - biosynthesis ; Potassium Channels - genetics ; Rats ; Rats, Sprague-Dawley ; Saponins - pharmacology ; Saponins - therapeutic use ; Triterpenes - pharmacology ; Triterpenes - therapeutic use ; Up-Regulation - drug effects</subject><ispartof>Life sciences (1973), 2016-01, Vol.144, p.94-102</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-3a20d642f9aa3af7f37dd29dddf4ead1d37e4aafb4b65c557019a75a5a427763</citedby><cites>FETCH-LOGICAL-c419t-3a20d642f9aa3af7f37dd29dddf4ead1d37e4aafb4b65c557019a75a5a427763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320515300849$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26593401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Xuejia</creatorcontrib><creatorcontrib>Guo, Qiao</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Yang, Xia</creatorcontrib><creatorcontrib>Tang, Yi-qun</creatorcontrib><title>Therapeutic effect of astragaloside-IV on bradycardia is involved in up-regulating klotho expression</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>In order to determine whether klotho is involved in the therapeutic effects of Astragaloside-IV on bradycardia, we evaluated the effect of ASG-IV on klotho and the effect of klotho on HCN4 and If.
Administrating isoproterenol (5mg/kg) for 15days to establish a rat bradycardia model randomized SD rats into control, model (ISO) and ASG-IV (5mg/kg/day) groups to explore the effect of ASG-IV on klotho. Rats were sacrificed on day15 after heart rate and heart function were measured; SAN tissues were collected to measure the expression of klotho and HCN4. In vitro, neonatal rat myocardial cells were incubated with LPS for 24h to inhibit the expression of HCN4 and incubated with LPS+ klotho to explore the effect of klotho on HCN4 expression. We also adopted full-patch-clamp technique to explore the effect of klotho on If.
Heart rate in model group was significantly decreased (356.6±19.7 vs. 428.9±19.9 in control group, P<0.01) and ASG-IV can increase heart rate (401.4±12.0 vs. 356.6±19.7 in model group, P<0.01). The expression of klotho was also up-regulated (P<0.05). In vitro, after incubation with LPS for 24h, HCN4 expression was significantly decreased in neonatal rat myocardial cells (0.6±0.07 vs. 1.0, P<0.01) and If was significantly declined. Exogenous klotho showed protective effect on HCN4 expression (1.58±0.16 in ASG-IV group vs. 0.6±0.07 in LPS group, P<0.05) and If.
Klotho is involved in the treatment mechanism of ASG-IV.
[Display omitted]</description><subject>Adrenergic beta-Agonists</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Astragaloside IV</subject><subject>Bradycardia</subject><subject>Bradycardia - chemically induced</subject><subject>Bradycardia - drug therapy</subject><subject>Bradycardia - genetics</subject><subject>Glucuronidase - biosynthesis</subject><subject>Glucuronidase - genetics</subject><subject>HCN4</subject><subject>Heart Function Tests</subject><subject>Heart Rate - drug effects</subject><subject>Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - biosynthesis</subject><subject>Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - genetics</subject><subject>ISO</subject><subject>Isoproterenol</subject><subject>Klotho</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Myocytes, Cardiac - drug effects</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channels - biosynthesis</subject><subject>Potassium Channels - genetics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Saponins - pharmacology</subject><subject>Saponins - therapeutic use</subject><subject>Triterpenes - pharmacology</subject><subject>Triterpenes - therapeutic use</subject><subject>Up-Regulation - drug effects</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAURS0EotPCD2CDvGST4OePmIgVqgqtVInNiK31Jn6eesjEwU5G9N_jagpLVu8tzr3SPYy9A9GCgO7joR1DaaUA0wK0QsILtoFPtm9Ep-Al2wghdaOkMBfsspSDEMIYq16zC9mZXmkBG-a3D5RxpnWJA6cQaFh4ChzLknGPYyrRU3P3g6eJ7zL6xwGzj8hj4XE6pfFEvj58nZtM-3XEJU57_nNMy0Pi9HvOVEpM0xv2KuBY6O3zvWLbrzfb69vm_vu3u-sv982goV8ahVL4TsvQIyoMNijrvey990ETevDKkkYMO73rzFCnCOjRGjSopbWdumIfzrVzTr9WKos7xjLQOOJEaS0OrNFGKmt1ReGMDjmVkim4Occj5kcHwj25dQdX3bontw7AVbc18_65ft0dyf9L_JVZgc9ngOrGU6TsyhBpGsjHXL06n-J_6v8A0DyL2Q</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Qiu, Xuejia</creator><creator>Guo, Qiao</creator><creator>Xiong, Wei</creator><creator>Yang, Xia</creator><creator>Tang, Yi-qun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160101</creationdate><title>Therapeutic effect of astragaloside-IV on bradycardia is involved in up-regulating klotho expression</title><author>Qiu, Xuejia ; Guo, Qiao ; Xiong, Wei ; Yang, Xia ; Tang, Yi-qun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-3a20d642f9aa3af7f37dd29dddf4ead1d37e4aafb4b65c557019a75a5a427763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adrenergic beta-Agonists</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Astragaloside IV</topic><topic>Bradycardia</topic><topic>Bradycardia - chemically induced</topic><topic>Bradycardia - drug therapy</topic><topic>Bradycardia - genetics</topic><topic>Glucuronidase - biosynthesis</topic><topic>Glucuronidase - genetics</topic><topic>HCN4</topic><topic>Heart Function Tests</topic><topic>Heart Rate - drug effects</topic><topic>Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - biosynthesis</topic><topic>Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - genetics</topic><topic>ISO</topic><topic>Isoproterenol</topic><topic>Klotho</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Myocytes, Cardiac - drug effects</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channels - biosynthesis</topic><topic>Potassium Channels - genetics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Saponins - pharmacology</topic><topic>Saponins - therapeutic use</topic><topic>Triterpenes - pharmacology</topic><topic>Triterpenes - therapeutic use</topic><topic>Up-Regulation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Xuejia</creatorcontrib><creatorcontrib>Guo, Qiao</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Yang, Xia</creatorcontrib><creatorcontrib>Tang, Yi-qun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Xuejia</au><au>Guo, Qiao</au><au>Xiong, Wei</au><au>Yang, Xia</au><au>Tang, Yi-qun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Therapeutic effect of astragaloside-IV on bradycardia is involved in up-regulating klotho expression</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>144</volume><spage>94</spage><epage>102</epage><pages>94-102</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>In order to determine whether klotho is involved in the therapeutic effects of Astragaloside-IV on bradycardia, we evaluated the effect of ASG-IV on klotho and the effect of klotho on HCN4 and If.
Administrating isoproterenol (5mg/kg) for 15days to establish a rat bradycardia model randomized SD rats into control, model (ISO) and ASG-IV (5mg/kg/day) groups to explore the effect of ASG-IV on klotho. Rats were sacrificed on day15 after heart rate and heart function were measured; SAN tissues were collected to measure the expression of klotho and HCN4. In vitro, neonatal rat myocardial cells were incubated with LPS for 24h to inhibit the expression of HCN4 and incubated with LPS+ klotho to explore the effect of klotho on HCN4 expression. We also adopted full-patch-clamp technique to explore the effect of klotho on If.
Heart rate in model group was significantly decreased (356.6±19.7 vs. 428.9±19.9 in control group, P<0.01) and ASG-IV can increase heart rate (401.4±12.0 vs. 356.6±19.7 in model group, P<0.01). The expression of klotho was also up-regulated (P<0.05). In vitro, after incubation with LPS for 24h, HCN4 expression was significantly decreased in neonatal rat myocardial cells (0.6±0.07 vs. 1.0, P<0.01) and If was significantly declined. Exogenous klotho showed protective effect on HCN4 expression (1.58±0.16 in ASG-IV group vs. 0.6±0.07 in LPS group, P<0.05) and If.
Klotho is involved in the treatment mechanism of ASG-IV.
[Display omitted]</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>26593401</pmid><doi>10.1016/j.lfs.2015.11.021</doi><tpages>9</tpages></addata></record> |
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| ispartof | Life sciences (1973), 2016-01, Vol.144, p.94-102 |
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| subjects | Adrenergic beta-Agonists Animals Animals, Newborn Astragaloside IV Bradycardia Bradycardia - chemically induced Bradycardia - drug therapy Bradycardia - genetics Glucuronidase - biosynthesis Glucuronidase - genetics HCN4 Heart Function Tests Heart Rate - drug effects Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - biosynthesis Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - genetics ISO Isoproterenol Klotho Lipopolysaccharides - pharmacology Myocytes, Cardiac - drug effects Myocytes, Cardiac - metabolism Patch-Clamp Techniques Potassium Channels - biosynthesis Potassium Channels - genetics Rats Rats, Sprague-Dawley Saponins - pharmacology Saponins - therapeutic use Triterpenes - pharmacology Triterpenes - therapeutic use Up-Regulation - drug effects |
| title | Therapeutic effect of astragaloside-IV on bradycardia is involved in up-regulating klotho expression |
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