Microsatellite amplification in Brassica napus cultivars: cultivar variability and relationship to a long-term feral population
Data from 83 Brassica microsatellites showed that null alleles, monomorphic loci and the amplification of multiple bands were relatively common. The data we have gathered here are essential for avoiding time consuming and costly optimization procedures. We used the data from 43 primers to assess the...
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| Veröffentlicht in: | Euphytica 2004, Vol.139 (2), p.173-178 |
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| creator | Bond, J.M Mogg, R.J Squire, G.R Johnstone, C |
| description | Data from 83 Brassica microsatellites showed that null alleles, monomorphic loci and the amplification of multiple bands were relatively common. The data we have gathered here are essential for avoiding time consuming and costly optimization procedures. We used the data from 43 primers to assess the relationship between 13 cultivars and one long-term feral population. The established feral population was found to be most similar to, but genetically differentiated from, the winter cultivars. Some feral plants clustered tightly with the winter cultivars, suggesting that only a small proportion of the population was generated from recently spilt seed. Since there was no similarity between the spring cultivars and the feral population, we suggest that a GM trait introduced into a spring cultivar is less likely to persist in the environment.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/s10681-004-2781-3 |
| format | Article |
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The data we have gathered here are essential for avoiding time consuming and costly optimization procedures. We used the data from 43 primers to assess the relationship between 13 cultivars and one long-term feral population. The established feral population was found to be most similar to, but genetically differentiated from, the winter cultivars. Some feral plants clustered tightly with the winter cultivars, suggesting that only a small proportion of the population was generated from recently spilt seed. Since there was no similarity between the spring cultivars and the feral population, we suggest that a GM trait introduced into a spring cultivar is less likely to persist in the environment.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0014-2336</identifier><identifier>EISSN: 1573-5060</identifier><identifier>DOI: 10.1007/s10681-004-2781-3</identifier><identifier>CODEN: EUPHAA</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agronomy. 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The data we have gathered here are essential for avoiding time consuming and costly optimization procedures. We used the data from 43 primers to assess the relationship between 13 cultivars and one long-term feral population. The established feral population was found to be most similar to, but genetically differentiated from, the winter cultivars. Some feral plants clustered tightly with the winter cultivars, suggesting that only a small proportion of the population was generated from recently spilt seed. Since there was no similarity between the spring cultivars and the feral population, we suggest that a GM trait introduced into a spring cultivar is less likely to persist in the environment.[PUBLICATION ABSTRACT]</description><subject>Agronomy. Soil science and plant productions</subject><subject>alleles</subject><subject>Biological and medical sciences</subject><subject>Brassica</subject><subject>Brassica napus</subject><subject>Cultivars</subject><subject>DNA primers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. 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Soil science and plant productions</topic><topic>alleles</topic><topic>Biological and medical sciences</topic><topic>Brassica</topic><topic>Brassica napus</topic><topic>Cultivars</topic><topic>DNA primers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. 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| subjects | Agronomy. Soil science and plant productions alleles Biological and medical sciences Brassica Brassica napus Cultivars DNA primers Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetic resources, diversity Genetics and breeding of economic plants microsatellite repeats Plant material polymerase chain reaction Spring spring cultivars wild relatives Winter winter cultivars |
| title | Microsatellite amplification in Brassica napus cultivars: cultivar variability and relationship to a long-term feral population |
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