Limits of detection of Mycobacterium tuberculosis in spiked cerebrospinal fluid using the polymerase chain reaction in tuberculous meningitis
The limit of detection of Mycobacterium tuberculosis in spiked cerebrospinal fluid (CSF) using polymerase chain reaction (PCR) was compared to that of a radiometric liquid culture. Serial dilutions of clinical isolates of Mycobacterium tuberculosis were prepared in CSF (n=3) or broth (n=11) with est...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2000-02, Vol.19 (1), p.47-50 |
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| creator | BARAN, J. JR RIEDERER, K. M KHATIB, R |
| description | The limit of detection of Mycobacterium tuberculosis in spiked cerebrospinal fluid (CSF) using polymerase chain reaction (PCR) was compared to that of a radiometric liquid culture. Serial dilutions of clinical isolates of Mycobacterium tuberculosis were prepared in CSF (n=3) or broth (n=11) with estimated concentrations of 0-550 cfu/ml. Each dilution was examined concurrently by PCR and radiometric culture. PCR and radiometric culture detected Mycobacterium tuberculosis DNA in all dilutions with an estimated 2 cfu/ml in the CSF. At lower concentrations (estimated |
| doi_str_mv | 10.1007/s100960050009 |
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These results imply that PCR can provide a rapid and reliable diagnosis of tuberculous meningitis, although there is a potential for false-negative results to occur in samples containing very few organisms ( < 2 cfu/ml).</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s100960050009</identifier><identifier>PMID: 10706180</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Bacterial diseases ; Bacterial diseases of the nervous system. 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JR</creatorcontrib><creatorcontrib>RIEDERER, K. M</creatorcontrib><creatorcontrib>KHATIB, R</creatorcontrib><title>Limits of detection of Mycobacterium tuberculosis in spiked cerebrospinal fluid using the polymerase chain reaction in tuberculous meningitis</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>The limit of detection of Mycobacterium tuberculosis in spiked cerebrospinal fluid (CSF) using polymerase chain reaction (PCR) was compared to that of a radiometric liquid culture. Serial dilutions of clinical isolates of Mycobacterium tuberculosis were prepared in CSF (n=3) or broth (n=11) with estimated concentrations of 0-550 cfu/ml. Each dilution was examined concurrently by PCR and radiometric culture. PCR and radiometric culture detected Mycobacterium tuberculosis DNA in all dilutions with an estimated 2 cfu/ml in the CSF. At lower concentrations (estimated <2 cfu/ml), PCR and radiometric culture were positive in three of five (60%) and five of five (100%) CSF samples, respectively. In comparison to PCR in broth dilutions, no evidence of inhibition or interference was noted. These results imply that PCR can provide a rapid and reliable diagnosis of tuberculous meningitis, although there is a potential for false-negative results to occur in samples containing very few organisms ( < 2 cfu/ml).</description><subject>Bacterial diseases</subject><subject>Bacterial diseases of the nervous system. 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Each dilution was examined concurrently by PCR and radiometric culture. PCR and radiometric culture detected Mycobacterium tuberculosis DNA in all dilutions with an estimated 2 cfu/ml in the CSF. At lower concentrations (estimated <2 cfu/ml), PCR and radiometric culture were positive in three of five (60%) and five of five (100%) CSF samples, respectively. In comparison to PCR in broth dilutions, no evidence of inhibition or interference was noted. These results imply that PCR can provide a rapid and reliable diagnosis of tuberculous meningitis, although there is a potential for false-negative results to occur in samples containing very few organisms ( < 2 cfu/ml).</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>10706180</pmid><doi>10.1007/s100960050009</doi><tpages>4</tpages></addata></record> |
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| subjects | Bacterial diseases Bacterial diseases of the nervous system. Bacterial myositis Biological and medical sciences Cerebrospinal fluid Cerebrospinal Fluid - microbiology Colony Count, Microbial Culture Media DNA, Bacterial - analysis Evaluation Studies as Topic Human bacterial diseases Humans Immunoassay Infectious diseases Laboratories Medical sciences Meningitis Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - growth & development Mycobacterium tuberculosis - isolation & purification Polymerase chain reaction Polymerase Chain Reaction - methods Radiometry Sensitivity and Specificity Tuberculosis Tuberculosis, Meningeal - diagnosis Tuberculosis, Meningeal - microbiology |
| title | Limits of detection of Mycobacterium tuberculosis in spiked cerebrospinal fluid using the polymerase chain reaction in tuberculous meningitis |
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