Isolation and characterization of microsatellite markers from Musa balbisiana
This is the first report of targeted development of B genome microsatellite markers in Musa. A total of 44 sequences with microsatellites were isolated from an enriched library of Musa balbisiana cv. ‘Tani’ (BB genome). Of these, 25 were polymorphic when screened on 14 diverse diploid and triploid M...
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Veröffentlicht in: | Molecular ecology notes 2005-06, Vol.5 (2), p.327-330 |
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description | This is the first report of targeted development of B genome microsatellite markers in Musa. A total of 44 sequences with microsatellites were isolated from an enriched library of Musa balbisiana cv. ‘Tani’ (BB genome). Of these, 25 were polymorphic when screened on 14 diverse diploid and triploid Musa accessions. The number of alleles detected by each marker ranged between one and seven. All 25 microsatellite markers generated amplification products in all species and genome complements. These new microsatellite markers fill an important gap for diversity assessment and linkage mapping studies in plantain (AAB) and cooking banana (ABB). |
doi_str_mv | 10.1111/j.1471-8286.2005.00916.x |
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These new microsatellite markers fill an important gap for diversity assessment and linkage mapping studies in plantain (AAB) and cooking banana (ABB).</description><subject>alleles</subject><subject>alleles per locus</subject><subject>B genome</subject><subject>DNA primers</subject><subject>genetic markers</subject><subject>heterozygosity</subject><subject>loci</subject><subject>microsatellite</subject><subject>microsatellite repeats</subject><subject>molecular sequence data</subject><subject>Musa</subject><subject>Musa balbisiana</subject><subject>nucleotide sequences</subject><subject>polymerase chain reaction</subject><subject>polymorphic loci</subject><subject>polymorphism</subject><subject>polyploid</subject><issn>1471-8278</issn><issn>1471-8286</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkMlOwzAQhiMEEuszkBO3BC_xEokLS1kEBSFAHEeT1AaXtAE7FYWnxyGoZ3yZkf1_I8-XJCklOY3ncJrTQtFMMy1zRojICSmpzJdrydbqYX3VK72ZbIcwJYSqkhVbyfgqtA12rp2nOJ-k9St6rDvj3fdw2dp05mrfBuxM07jOpDP0b8aH1Pp2lo4XAdMKm8oFh3PcTTYsNsHs_dWd5Ol89Hh6md3cXVydHt9kdSGEzLSupLG8ElroigithMRaMo3WYjmpiCwZF5WUVtraUi6wmsQyKS0tbFkwzXeSg2Huu28_FiZ0MHOhjh_EuWkXAagSXClexKAegv0KwRsL797FDb6AEuj9wRR6NdBrgt4f_PqDZUSPBvTTNebr3xyMR7exiXg24C50ZrnCozyQiisBz7cXcH1_dqIvHxRcx_z-kLfYAr54F-DpgRHKCSWECSb4D3kQj5U</recordid><startdate>200506</startdate><enddate>200506</enddate><creator>Buhariwalla, H.K</creator><creator>Jarret, R.L</creator><creator>Jayashree B</creator><creator>Crouch, J.H</creator><creator>Ortiz, R</creator><general>Blackwell Science Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200506</creationdate><title>Isolation and characterization of microsatellite markers from Musa balbisiana</title><author>Buhariwalla, H.K ; Jarret, R.L ; Jayashree B ; Crouch, J.H ; Ortiz, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4556-88b6ef3b5858b058756ac628affa9db069235b66f6fcf135abdf13d9f14f94283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>alleles</topic><topic>alleles per locus</topic><topic>B genome</topic><topic>DNA primers</topic><topic>genetic markers</topic><topic>heterozygosity</topic><topic>loci</topic><topic>microsatellite</topic><topic>microsatellite repeats</topic><topic>molecular sequence data</topic><topic>Musa</topic><topic>Musa balbisiana</topic><topic>nucleotide sequences</topic><topic>polymerase chain reaction</topic><topic>polymorphic loci</topic><topic>polymorphism</topic><topic>polyploid</topic><toplevel>online_resources</toplevel><creatorcontrib>Buhariwalla, H.K</creatorcontrib><creatorcontrib>Jarret, R.L</creatorcontrib><creatorcontrib>Jayashree B</creatorcontrib><creatorcontrib>Crouch, J.H</creatorcontrib><creatorcontrib>Ortiz, R</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular ecology notes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buhariwalla, H.K</au><au>Jarret, R.L</au><au>Jayashree B</au><au>Crouch, J.H</au><au>Ortiz, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of microsatellite markers from Musa balbisiana</atitle><jtitle>Molecular ecology notes</jtitle><date>2005-06</date><risdate>2005</risdate><volume>5</volume><issue>2</issue><spage>327</spage><epage>330</epage><pages>327-330</pages><issn>1471-8278</issn><eissn>1471-8286</eissn><abstract>This is the first report of targeted development of B genome microsatellite markers in Musa. 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subjects | alleles alleles per locus B genome DNA primers genetic markers heterozygosity loci microsatellite microsatellite repeats molecular sequence data Musa Musa balbisiana nucleotide sequences polymerase chain reaction polymorphic loci polymorphism polyploid |
title | Isolation and characterization of microsatellite markers from Musa balbisiana |
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