Isolation and characterization of microsatellite markers from Musa balbisiana

This is the first report of targeted development of B genome microsatellite markers in Musa. A total of 44 sequences with microsatellites were isolated from an enriched library of Musa balbisiana cv. ‘Tani’ (BB genome). Of these, 25 were polymorphic when screened on 14 diverse diploid and triploid M...

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Veröffentlicht in:Molecular ecology notes 2005-06, Vol.5 (2), p.327-330
Hauptverfasser: Buhariwalla, H.K, Jarret, R.L, Jayashree B, Crouch, J.H, Ortiz, R
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container_title Molecular ecology notes
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creator Buhariwalla, H.K
Jarret, R.L
Jayashree B
Crouch, J.H
Ortiz, R
description This is the first report of targeted development of B genome microsatellite markers in Musa. A total of 44 sequences with microsatellites were isolated from an enriched library of Musa balbisiana cv. ‘Tani’ (BB genome). Of these, 25 were polymorphic when screened on 14 diverse diploid and triploid Musa accessions. The number of alleles detected by each marker ranged between one and seven. All 25 microsatellite markers generated amplification products in all species and genome complements. These new microsatellite markers fill an important gap for diversity assessment and linkage mapping studies in plantain (AAB) and cooking banana (ABB).
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subjects alleles
alleles per locus
B genome
DNA primers
genetic markers
heterozygosity
loci
microsatellite
microsatellite repeats
molecular sequence data
Musa
Musa balbisiana
nucleotide sequences
polymerase chain reaction
polymorphic loci
polymorphism
polyploid
title Isolation and characterization of microsatellite markers from Musa balbisiana
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