Recombinant chromosomes of advanced backcross plants between Allium cepa L. and A. fistulosum L. revealed by in situ hybridization

Cytological analysis of (Allium cepa L. x Allium fistulosum L.) x A. cepa L. [F.sub.1][BC.sub.3] plants revealed most plants were diploid with 16 chromosomes. Karyotypes of these plants showed recombinant chromosomes. Fluorescence and genomic in situ hybridization patterns of interspecific [F.sub.1]...

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Veröffentlicht in:	Theoretical and applied genetics 2000-06, Vol.100 (8), p.1190-1196
Hauptverfasser:	HOU, A, PEFFLEY, E. B
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Allium cepa Allium fistulosum Biological and medical sciences Chromosomes Fertility Fundamental and applied biological sciences. Psychology Genetic aspects Genetic variation Genetics and breeding of economic plants Genomes Hybridization Interspecific and intergeneric hybridization, introgressions Morphology Onions Physiological aspects Plant breeding: fundamental aspects and methodology Plant genetics Soil sciences
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Zusammenfassung:	Cytological analysis of (Allium cepa L. x Allium fistulosum L.) x A. cepa L. [F.sub.1][BC.sub.3] plants revealed most plants were diploid with 16 chromosomes. Karyotypes of these plants showed recombinant chromosomes. Fluorescence and genomic in situ hybridization patterns of interspecific [F.sub.1] hybrid and [F.sub.1][BC.sub.3] plants revealed A. fistulosum chromosomes or chromosomal segments. A highly repetitive 376-bp DNA sequence and genomic DNA of A. fistulosum revealed similar telomeric hybridization sites when hybridized onto A. fistulosum chromosomes. Cytogenetic evidence showed that A. fistulosum DNA has recombined into the A. cepa genome.
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s001220051423