Cytotoxicity of silica nanoparticles on HaCaT cells
ABSTRACT Despite the widespread use of silica nanoparticles (SiO2 NPs) in biological and medical fields, their adverse effects have not been clearly elucidated. In this study, spherical SiO2 NPs with a 50 nm diameter were used to study their interaction with HaCaT cells. SiO2 NPs were found to be re...
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| Veröffentlicht in: | Journal of applied toxicology 2014-04, Vol.34 (4), p.367-372 |
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| creator | Liang, Hao Jin, Chan Tang, Ying Wang, Fude Ma, Chunwang Yang, Yongji |
| description | ABSTRACT
Despite the widespread use of silica nanoparticles (SiO2 NPs) in biological and medical fields, their adverse effects have not been clearly elucidated. In this study, spherical SiO2 NPs with a 50 nm diameter were used to study their interaction with HaCaT cells. SiO2 NPs were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant glutathione depletion and reactive oxygen species generation, which reduced the cellular antioxidant level, could be the major factor of cytotoxicity induced by SiO2 NPs. Copyright © 2013 John Wiley & Sons, Ltd.
Spherical SiO2 nanoparticles (SiO2 NPs) with a 50 nm diameter were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes, and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant GSH depletion and ROS generation which reduced the cellular antioxidant level could be the major factor of cytotoxicity induced by SiO2 NPs. |
| doi_str_mv | 10.1002/jat.2953 |
| format | Article |
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Despite the widespread use of silica nanoparticles (SiO2 NPs) in biological and medical fields, their adverse effects have not been clearly elucidated. In this study, spherical SiO2 NPs with a 50 nm diameter were used to study their interaction with HaCaT cells. SiO2 NPs were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant glutathione depletion and reactive oxygen species generation, which reduced the cellular antioxidant level, could be the major factor of cytotoxicity induced by SiO2 NPs. Copyright © 2013 John Wiley & Sons, Ltd.
Spherical SiO2 nanoparticles (SiO2 NPs) with a 50 nm diameter were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes, and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant GSH depletion and ROS generation which reduced the cellular antioxidant level could be the major factor of cytotoxicity induced by SiO2 NPs.</description><identifier>ISSN: 0260-437X</identifier><identifier>EISSN: 1099-1263</identifier><identifier>DOI: 10.1002/jat.2953</identifier><identifier>PMID: 24155225</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Antioxidants ; Autophagy - drug effects ; Cell Culture Techniques ; Cell Line ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell Membrane - ultrastructure ; Cell Survival - drug effects ; Cellular ; Cytoplasm - drug effects ; Cytoplasm - metabolism ; Cytoplasm - ultrastructure ; Cytotoxicity ; Damage ; Depletion ; Dose-Response Relationship, Drug ; HaCaT cells ; Humans ; Integrity ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Keratinocytes - ultrastructure ; Lysosomes ; Lysosomes - drug effects ; Lysosomes - metabolism ; Lysosomes - ultrastructure ; Microscopy, Confocal ; Microscopy, Electron, Transmission ; Nanoparticles ; Nanoparticles - chemistry ; Oxidative stress ; Oxidative Stress - drug effects ; Silica ; Silicon dioxide ; Silicon Dioxide - chemistry ; Silicon Dioxide - toxicity ; Silicon dioxide nanoparticles ; Transmission electron microscope (TEM)</subject><ispartof>Journal of applied toxicology, 2014-04, Vol.34 (4), p.367-372</ispartof><rights>Copyright © 2013 John Wiley & Sons, Ltd.</rights><rights>Copyright © 2014 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5533-3fe934f484851829b11488a31e0d36a7c4285af44632d10e9570019c9a8e46d03</citedby><cites>FETCH-LOGICAL-c5533-3fe934f484851829b11488a31e0d36a7c4285af44632d10e9570019c9a8e46d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjat.2953$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjat.2953$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24155225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Hao</creatorcontrib><creatorcontrib>Jin, Chan</creatorcontrib><creatorcontrib>Tang, Ying</creatorcontrib><creatorcontrib>Wang, Fude</creatorcontrib><creatorcontrib>Ma, Chunwang</creatorcontrib><creatorcontrib>Yang, Yongji</creatorcontrib><title>Cytotoxicity of silica nanoparticles on HaCaT cells</title><title>Journal of applied toxicology</title><addtitle>J. Appl. Toxicol</addtitle><description>ABSTRACT
Despite the widespread use of silica nanoparticles (SiO2 NPs) in biological and medical fields, their adverse effects have not been clearly elucidated. In this study, spherical SiO2 NPs with a 50 nm diameter were used to study their interaction with HaCaT cells. SiO2 NPs were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant glutathione depletion and reactive oxygen species generation, which reduced the cellular antioxidant level, could be the major factor of cytotoxicity induced by SiO2 NPs. Copyright © 2013 John Wiley & Sons, Ltd.
Spherical SiO2 nanoparticles (SiO2 NPs) with a 50 nm diameter were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes, and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant GSH depletion and ROS generation which reduced the cellular antioxidant level could be the major factor of cytotoxicity induced by SiO2 NPs.</description><subject>Antioxidants</subject><subject>Autophagy - drug effects</subject><subject>Cell Culture Techniques</subject><subject>Cell Line</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Survival - drug effects</subject><subject>Cellular</subject><subject>Cytoplasm - drug effects</subject><subject>Cytoplasm - metabolism</subject><subject>Cytoplasm - ultrastructure</subject><subject>Cytotoxicity</subject><subject>Damage</subject><subject>Depletion</subject><subject>Dose-Response Relationship, Drug</subject><subject>HaCaT cells</subject><subject>Humans</subject><subject>Integrity</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - ultrastructure</subject><subject>Lysosomes</subject><subject>Lysosomes - drug effects</subject><subject>Lysosomes - metabolism</subject><subject>Lysosomes - ultrastructure</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron, Transmission</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Silicon Dioxide - chemistry</subject><subject>Silicon Dioxide - toxicity</subject><subject>Silicon dioxide nanoparticles</subject><subject>Transmission electron microscope (TEM)</subject><issn>0260-437X</issn><issn>1099-1263</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1Lw0AQQBdRbP0Af4EEvHhJndmPZPeoQVtFFKSieFm26Qa2ptmaTbH996a0VhDE0xzm8Zh5hJwg9BCAXkxM06NKsB3SRVAqRpqwXdIFmkDMWfraIQchTADaHZX7pEM5CkGp6BKWLRvf-IXLXbOMfBEFV7rcRJWp_MzUjctLGyJfRQOTmWGU27IMR2SvMGWwx5t5SJ5vrofZIL5_7N9ml_dxLgRjMSusYrzgkkuBkqoRIpfSMLQwZolJc06lMAXnCaNjBKtECoAqV0ZanoyBHZLztXdW-4-5DY2eurC6wFTWz4PGVDAhELj8HxUAadKes0LPfqETP6-r9hGNXFEUqGT6I8xrH0JtCz2r3dTUS42gV81121yvmrfo6UY4H03teAt-R26BeA18utIu_xTpu8vhRrjhXWjsYsub-l0nKUuFfnno6zdOnyBTV7rPvgDDEZVT</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Liang, Hao</creator><creator>Jin, Chan</creator><creator>Tang, Ying</creator><creator>Wang, Fude</creator><creator>Ma, Chunwang</creator><creator>Yang, Yongji</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>K9.</scope><scope>SOI</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope><scope>L7M</scope></search><sort><creationdate>201404</creationdate><title>Cytotoxicity of silica nanoparticles on HaCaT cells</title><author>Liang, Hao ; Jin, Chan ; Tang, Ying ; Wang, Fude ; Ma, Chunwang ; Yang, Yongji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5533-3fe934f484851829b11488a31e0d36a7c4285af44632d10e9570019c9a8e46d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antioxidants</topic><topic>Autophagy - drug effects</topic><topic>Cell Culture Techniques</topic><topic>Cell Line</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell Survival - drug effects</topic><topic>Cellular</topic><topic>Cytoplasm - drug effects</topic><topic>Cytoplasm - metabolism</topic><topic>Cytoplasm - ultrastructure</topic><topic>Cytotoxicity</topic><topic>Damage</topic><topic>Depletion</topic><topic>Dose-Response Relationship, Drug</topic><topic>HaCaT cells</topic><topic>Humans</topic><topic>Integrity</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Keratinocytes - ultrastructure</topic><topic>Lysosomes</topic><topic>Lysosomes - drug effects</topic><topic>Lysosomes - metabolism</topic><topic>Lysosomes - ultrastructure</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Electron, Transmission</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Silica</topic><topic>Silicon dioxide</topic><topic>Silicon Dioxide - chemistry</topic><topic>Silicon Dioxide - toxicity</topic><topic>Silicon dioxide nanoparticles</topic><topic>Transmission electron microscope (TEM)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Hao</creatorcontrib><creatorcontrib>Jin, Chan</creatorcontrib><creatorcontrib>Tang, Ying</creatorcontrib><creatorcontrib>Wang, Fude</creatorcontrib><creatorcontrib>Ma, Chunwang</creatorcontrib><creatorcontrib>Yang, Yongji</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Environment Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of applied toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Hao</au><au>Jin, Chan</au><au>Tang, Ying</au><au>Wang, Fude</au><au>Ma, Chunwang</au><au>Yang, Yongji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity of silica nanoparticles on HaCaT cells</atitle><jtitle>Journal of applied toxicology</jtitle><addtitle>J. Appl. Toxicol</addtitle><date>2014-04</date><risdate>2014</risdate><volume>34</volume><issue>4</issue><spage>367</spage><epage>372</epage><pages>367-372</pages><issn>0260-437X</issn><eissn>1099-1263</eissn><abstract>ABSTRACT
Despite the widespread use of silica nanoparticles (SiO2 NPs) in biological and medical fields, their adverse effects have not been clearly elucidated. In this study, spherical SiO2 NPs with a 50 nm diameter were used to study their interaction with HaCaT cells. SiO2 NPs were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant glutathione depletion and reactive oxygen species generation, which reduced the cellular antioxidant level, could be the major factor of cytotoxicity induced by SiO2 NPs. Copyright © 2013 John Wiley & Sons, Ltd.
Spherical SiO2 nanoparticles (SiO2 NPs) with a 50 nm diameter were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes, and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant GSH depletion and ROS generation which reduced the cellular antioxidant level could be the major factor of cytotoxicity induced by SiO2 NPs.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24155225</pmid><doi>10.1002/jat.2953</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antioxidants Autophagy - drug effects Cell Culture Techniques Cell Line Cell Membrane - drug effects Cell Membrane - metabolism Cell Membrane - ultrastructure Cell Survival - drug effects Cellular Cytoplasm - drug effects Cytoplasm - metabolism Cytoplasm - ultrastructure Cytotoxicity Damage Depletion Dose-Response Relationship, Drug HaCaT cells Humans Integrity Keratinocytes - drug effects Keratinocytes - metabolism Keratinocytes - ultrastructure Lysosomes Lysosomes - drug effects Lysosomes - metabolism Lysosomes - ultrastructure Microscopy, Confocal Microscopy, Electron, Transmission Nanoparticles Nanoparticles - chemistry Oxidative stress Oxidative Stress - drug effects Silica Silicon dioxide Silicon Dioxide - chemistry Silicon Dioxide - toxicity Silicon dioxide nanoparticles Transmission electron microscope (TEM) |
| title | Cytotoxicity of silica nanoparticles on HaCaT cells |
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