The correlations between alteration of p16 gene and clinicopathological factors and prognosis in squamous cell carcinomas of the buccal mucosa
J Oral Pathol Med (2012) 41: 463–469 Objective: To evaluate relationships between the alteration of p16 gene and the clinical status and prognosis of the patients with squamous cell carcinoma of the buccal mucosa. Methods: Thirty buccal cancers were included in the analysis. Deletion analysis was...
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| creator | Dong, Yuying Wang, Jie Dong, Fusheng Wang, Xu Zhang, Yinghuai |
| description | J Oral Pathol Med (2012) 41: 463–469
Objective: To evaluate relationships between the alteration of p16 gene and the clinical status and prognosis of the patients with squamous cell carcinoma of the buccal mucosa.
Methods: Thirty buccal cancers were included in the analysis. Deletion analysis was performed by PCR. Point mutation analysis was used by PCR‐SSCP and direct sequencing. Methylation‐specific PCR methods were adopted for the evaluation of p16 methylation. The correlation between alteration of p16 gene and clinicopathological factors buccal cancer was evaluated by Fisher’s exact test. Kaplan–Meier and Cox regression were used to investigate the relationship between p16 alteration and survival time.
Results: The frequency of p16 alteration was 63.3% in buccal carcinomas. P16 deletion was associated significantly with tumor size (P = 0.01). P16 point mutation was associated significantly with differentiation (P = 0.006). P16 methylation was associated significantly with nodes metastasis (P = 0.027). The overall survival rate of 30 buccal carcinomas was 53.3%. The Log‐rank test (P = 0.021) and univariate Cox regression analysis (P = 0.030) revealed that p16 methylation was significantly associated with the overall survival rate. Multivariate analysis showed that p16 deletion, p16 mutation, and p16 methylation were not statistically significant.
Conclusions: The alterations of p16 gene may play a major role in malignancy and development and metastases of buccal carcinoma and may be an excellent marker of aggressive clinical behavior. P16 methylation has a prognostic value in buccal carcinoma but not an independent prognosis factor. P16 point mutation and p16 deletion have not prognostic significance in buccal carcinoma. |
| doi_str_mv | 10.1111/j.1600-0714.2012.01132.x |
| format | Article |
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Objective: To evaluate relationships between the alteration of p16 gene and the clinical status and prognosis of the patients with squamous cell carcinoma of the buccal mucosa.
Methods: Thirty buccal cancers were included in the analysis. Deletion analysis was performed by PCR. Point mutation analysis was used by PCR‐SSCP and direct sequencing. Methylation‐specific PCR methods were adopted for the evaluation of p16 methylation. The correlation between alteration of p16 gene and clinicopathological factors buccal cancer was evaluated by Fisher’s exact test. Kaplan–Meier and Cox regression were used to investigate the relationship between p16 alteration and survival time.
Results: The frequency of p16 alteration was 63.3% in buccal carcinomas. P16 deletion was associated significantly with tumor size (P = 0.01). P16 point mutation was associated significantly with differentiation (P = 0.006). P16 methylation was associated significantly with nodes metastasis (P = 0.027). The overall survival rate of 30 buccal carcinomas was 53.3%. The Log‐rank test (P = 0.021) and univariate Cox regression analysis (P = 0.030) revealed that p16 methylation was significantly associated with the overall survival rate. Multivariate analysis showed that p16 deletion, p16 mutation, and p16 methylation were not statistically significant.
Conclusions: The alterations of p16 gene may play a major role in malignancy and development and metastases of buccal carcinoma and may be an excellent marker of aggressive clinical behavior. P16 methylation has a prognostic value in buccal carcinoma but not an independent prognosis factor. P16 point mutation and p16 deletion have not prognostic significance in buccal carcinoma.</description><identifier>ISSN: 0904-2512</identifier><identifier>EISSN: 1600-0714</identifier><identifier>DOI: 10.1111/j.1600-0714.2012.01132.x</identifier><identifier>PMID: 22429295</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Biological and medical sciences ; buccal mucosa ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - secondary ; Cell Differentiation - genetics ; Cell Nucleus - ultrastructure ; Cytoplasm - ultrastructure ; Dentistry ; Dermatology ; DNA Methylation ; Exons - genetics ; Female ; Follow-Up Studies ; Gene Deletion ; Genes, p16 ; Homozygote ; homozygous deletion ; Humans ; Lymphatic Metastasis - genetics ; Lymphatic Metastasis - pathology ; Male ; Medical sciences ; methylation ; Middle Aged ; Mouth Mucosa - pathology ; Mouth Neoplasms - genetics ; Mouth Neoplasms - pathology ; Neoplasm Staging ; Otorhinolaryngology. Stomatology ; p16 gene ; point mutation ; Point Mutation - genetics ; Polymorphism, Single-Stranded Conformational - genetics ; Prognosis ; Promoter Regions, Genetic - genetics ; Sequence Analysis, DNA ; squamous cell carcinoma ; Survival Rate ; Tumors of the skin and soft tissue. Premalignant lesions</subject><ispartof>Journal of oral pathology & medicine, 2012-07, Vol.41 (6), p.463-469</ispartof><rights>2012 John Wiley & Sons A/S</rights><rights>2015 INIST-CNRS</rights><rights>2012 John Wiley & Sons A/S.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5362-70bf36fb027dcdb34401656b8e564410b514558172083fc1cbb48562be24e3c53</citedby><cites>FETCH-LOGICAL-c5362-70bf36fb027dcdb34401656b8e564410b514558172083fc1cbb48562be24e3c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0714.2012.01132.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0714.2012.01132.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26103407$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22429295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Yuying</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Dong, Fusheng</creatorcontrib><creatorcontrib>Wang, Xu</creatorcontrib><creatorcontrib>Zhang, Yinghuai</creatorcontrib><title>The correlations between alteration of p16 gene and clinicopathological factors and prognosis in squamous cell carcinomas of the buccal mucosa</title><title>Journal of oral pathology & medicine</title><addtitle>J Oral Pathol Med</addtitle><description>J Oral Pathol Med (2012) 41: 463–469
Objective: To evaluate relationships between the alteration of p16 gene and the clinical status and prognosis of the patients with squamous cell carcinoma of the buccal mucosa.
Methods: Thirty buccal cancers were included in the analysis. Deletion analysis was performed by PCR. Point mutation analysis was used by PCR‐SSCP and direct sequencing. Methylation‐specific PCR methods were adopted for the evaluation of p16 methylation. The correlation between alteration of p16 gene and clinicopathological factors buccal cancer was evaluated by Fisher’s exact test. Kaplan–Meier and Cox regression were used to investigate the relationship between p16 alteration and survival time.
Results: The frequency of p16 alteration was 63.3% in buccal carcinomas. P16 deletion was associated significantly with tumor size (P = 0.01). P16 point mutation was associated significantly with differentiation (P = 0.006). P16 methylation was associated significantly with nodes metastasis (P = 0.027). The overall survival rate of 30 buccal carcinomas was 53.3%. The Log‐rank test (P = 0.021) and univariate Cox regression analysis (P = 0.030) revealed that p16 methylation was significantly associated with the overall survival rate. Multivariate analysis showed that p16 deletion, p16 mutation, and p16 methylation were not statistically significant.
Conclusions: The alterations of p16 gene may play a major role in malignancy and development and metastases of buccal carcinoma and may be an excellent marker of aggressive clinical behavior. P16 methylation has a prognostic value in buccal carcinoma but not an independent prognosis factor. P16 point mutation and p16 deletion have not prognostic significance in buccal carcinoma.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>buccal mucosa</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - secondary</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cytoplasm - ultrastructure</subject><subject>Dentistry</subject><subject>Dermatology</subject><subject>DNA Methylation</subject><subject>Exons - genetics</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gene Deletion</subject><subject>Genes, p16</subject><subject>Homozygote</subject><subject>homozygous deletion</subject><subject>Humans</subject><subject>Lymphatic Metastasis - genetics</subject><subject>Lymphatic Metastasis - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>methylation</subject><subject>Middle Aged</subject><subject>Mouth Mucosa - pathology</subject><subject>Mouth Neoplasms - genetics</subject><subject>Mouth Neoplasms - pathology</subject><subject>Neoplasm Staging</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>p16 gene</subject><subject>point mutation</subject><subject>Point Mutation - genetics</subject><subject>Polymorphism, Single-Stranded Conformational - genetics</subject><subject>Prognosis</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>squamous cell carcinoma</subject><subject>Survival Rate</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><issn>0904-2512</issn><issn>1600-0714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEUhS0EoqHwCsgbJDYTfP03kwULqGgBVS0SRbCzbMeTOszYqT2jpi_BM-NJQliCN7Z8v3Pv0T0IYSBzKOfNeg6SkIrUwOeUAJ0TAEbn20dodiw8RjOyILyiAugJepbzmhCoGYen6IRSThd0IWbo182twzam5Do9-BgyNm64dy5g3Q0u7f5wbPEGJF654LAOS2w7H7yNGz3cxi6uvNUdbrUdYsq7-ibFVYjZZ-wDznej7uOYsXVdh61O1ofY6zx1HcpwM9pJ3482Zv0cPWl1l92Lw32Kvp1_uDn7WF1eX3w6e3dZWcEkrWpiWiZbQ2i9tEvDOCcghTSNE5JzIEYAF6KBmpKGtRasMbwRkhpHuWOlxyl6ve9brN6NLg-q93kyqIMrXhXUggkGsuH_RgktaFkmFLTZozbFnJNr1Sb5XqeHAqkpOLVWUz5qykdNwaldcGpbpC8PU0bTu-VR-CepArw6ADqXfbVJB-vzX04CYZzUhXu75-595x7-24D6fP1lehV9tdf7PLjtUa_TTyVrVgv1_epCySvC6fmPr-o9-w2Cg8Mr</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Dong, Yuying</creator><creator>Wang, Jie</creator><creator>Dong, Fusheng</creator><creator>Wang, Xu</creator><creator>Zhang, Yinghuai</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201207</creationdate><title>The correlations between alteration of p16 gene and clinicopathological factors and prognosis in squamous cell carcinomas of the buccal mucosa</title><author>Dong, Yuying ; Wang, Jie ; Dong, Fusheng ; Wang, Xu ; Zhang, Yinghuai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5362-70bf36fb027dcdb34401656b8e564410b514558172083fc1cbb48562be24e3c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>buccal mucosa</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - secondary</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cytoplasm - ultrastructure</topic><topic>Dentistry</topic><topic>Dermatology</topic><topic>DNA Methylation</topic><topic>Exons - genetics</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Gene Deletion</topic><topic>Genes, p16</topic><topic>Homozygote</topic><topic>homozygous deletion</topic><topic>Humans</topic><topic>Lymphatic Metastasis - genetics</topic><topic>Lymphatic Metastasis - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>methylation</topic><topic>Middle Aged</topic><topic>Mouth Mucosa - pathology</topic><topic>Mouth Neoplasms - genetics</topic><topic>Mouth Neoplasms - pathology</topic><topic>Neoplasm Staging</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>p16 gene</topic><topic>point mutation</topic><topic>Point Mutation - genetics</topic><topic>Polymorphism, Single-Stranded Conformational - genetics</topic><topic>Prognosis</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>squamous cell carcinoma</topic><topic>Survival Rate</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Yuying</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Dong, Fusheng</creatorcontrib><creatorcontrib>Wang, Xu</creatorcontrib><creatorcontrib>Zhang, Yinghuai</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of oral pathology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Yuying</au><au>Wang, Jie</au><au>Dong, Fusheng</au><au>Wang, Xu</au><au>Zhang, Yinghuai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The correlations between alteration of p16 gene and clinicopathological factors and prognosis in squamous cell carcinomas of the buccal mucosa</atitle><jtitle>Journal of oral pathology & medicine</jtitle><addtitle>J Oral Pathol Med</addtitle><date>2012-07</date><risdate>2012</risdate><volume>41</volume><issue>6</issue><spage>463</spage><epage>469</epage><pages>463-469</pages><issn>0904-2512</issn><eissn>1600-0714</eissn><abstract>J Oral Pathol Med (2012) 41: 463–469
Objective: To evaluate relationships between the alteration of p16 gene and the clinical status and prognosis of the patients with squamous cell carcinoma of the buccal mucosa.
Methods: Thirty buccal cancers were included in the analysis. Deletion analysis was performed by PCR. Point mutation analysis was used by PCR‐SSCP and direct sequencing. Methylation‐specific PCR methods were adopted for the evaluation of p16 methylation. The correlation between alteration of p16 gene and clinicopathological factors buccal cancer was evaluated by Fisher’s exact test. Kaplan–Meier and Cox regression were used to investigate the relationship between p16 alteration and survival time.
Results: The frequency of p16 alteration was 63.3% in buccal carcinomas. P16 deletion was associated significantly with tumor size (P = 0.01). P16 point mutation was associated significantly with differentiation (P = 0.006). P16 methylation was associated significantly with nodes metastasis (P = 0.027). The overall survival rate of 30 buccal carcinomas was 53.3%. The Log‐rank test (P = 0.021) and univariate Cox regression analysis (P = 0.030) revealed that p16 methylation was significantly associated with the overall survival rate. Multivariate analysis showed that p16 deletion, p16 mutation, and p16 methylation were not statistically significant.
Conclusions: The alterations of p16 gene may play a major role in malignancy and development and metastases of buccal carcinoma and may be an excellent marker of aggressive clinical behavior. P16 methylation has a prognostic value in buccal carcinoma but not an independent prognosis factor. P16 point mutation and p16 deletion have not prognostic significance in buccal carcinoma.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22429295</pmid><doi>10.1111/j.1600-0714.2012.01132.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged Biological and medical sciences buccal mucosa Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - secondary Cell Differentiation - genetics Cell Nucleus - ultrastructure Cytoplasm - ultrastructure Dentistry Dermatology DNA Methylation Exons - genetics Female Follow-Up Studies Gene Deletion Genes, p16 Homozygote homozygous deletion Humans Lymphatic Metastasis - genetics Lymphatic Metastasis - pathology Male Medical sciences methylation Middle Aged Mouth Mucosa - pathology Mouth Neoplasms - genetics Mouth Neoplasms - pathology Neoplasm Staging Otorhinolaryngology. Stomatology p16 gene point mutation Point Mutation - genetics Polymorphism, Single-Stranded Conformational - genetics Prognosis Promoter Regions, Genetic - genetics Sequence Analysis, DNA squamous cell carcinoma Survival Rate Tumors of the skin and soft tissue. Premalignant lesions |
| title | The correlations between alteration of p16 gene and clinicopathological factors and prognosis in squamous cell carcinomas of the buccal mucosa |
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