Ultrasonic and Densimetric Characterization of the Association of Cyclic AMP with the cAMP-Binding Domain of the Exchange Protein EPAC1

We employed a combination of densimetric and ultrasonic velocimetric techniques to characterize the volumetric properties of the association of the cAMP-binding domain (CBD) of EPAC1 with cAMP at 25 °C in a pH 7.6 buffer. The binding of cAMP to the CBD of EPAC1 is accompanied by changes in volume, Δ...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The journal of physical chemistry. B 2013-09, Vol.117 (37), p.10779-10784
Hauptverfasser:	Son, Ikbae, Selvaratnam, Rajeevan, Dubins, David N, Melacini, Giuseppe, Chalikian, Tigran V
Format:	Artikel
Sprache:	eng
Schlagworte:	Adiabatic flow Binding Binding Sites Biological and medical sciences Compressibility Cyclic AMP - metabolism Dynamics Entropy Estimates Fundamental and applied biological sciences. Psychology Guanine Nucleotide Exchange Factors - chemistry Guanine Nucleotide Exchange Factors - metabolism Hydration Hydrogen-Ion Concentration Intermolecular phenomena Molecular biophysics Protein Conformation Proteins Thermodynamics Ultrasonics Water
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!